We narrowed to 42,011 results for: LAS

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mc2 blast EF1a

Plasmid

#206223

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-mScarletI

Plasmid

#201442

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mScarlet-I

Use

Unspecified

Promoter

read through

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pEXP5-NT/pLux LasI

Plasmid

#193628

Purpose

Inducible expression of LasI by the transcription factor complex LuxR-3OC6 homoserine lactone (HSL). LasI is codon optimized for E. coli.

Depositor

Dora Tang

Article

Gonzales et al ChemSystemsChem 2023

Insert

LasI

Tags

6xHis

Expression

Bacterial

Promoter

Lux promoter

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv6-blast-His:mScarletI:His

Plasmid

#201097

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mScarlet-I

Use

Unspecified

Promoter

read through

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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LentiCas9-sgCh2-2-Blast

Plasmid

#199645

Purpose

Expresses Cas9 and sgRNA control guide targeting intergenic region

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

N/A

Use

Lentiviral

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-BFP

Plasmid

#190258

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Sue Vaughan

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

BFP

Use

For endogenous gene tagging

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-spGFP(1-10)

Plasmid

#190208

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

spGFP(1-10)

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-mNG2(1-10)

Plasmid

#190209

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mNeonGreen2(1-10)

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-mScarlet

Plasmid

#179818

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mScarlet

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-10Ty

Plasmid

#179807

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

10xTy

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-SNAPtag

Plasmid

#179786

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

SNAP

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-birA*

Plasmid

#179788

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

birA

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti_EGFP-P2A-siRNAresHAUS6_IRES_Blast

Plasmid

#182887

Purpose

Transfer vector for production of lentivirus. Expresses EGFP-P2A-HAUS6 (siRNA resistant version)

Depositor

Daniel Gerlich

Article

David et al J Cell Biol. 2019 Jul 1;218(7):2150-2168

Insert

HAUS 6 (HAUS6 Human)

Use

Lentiviral

Tags

EGFP

Expression

Bacterial and Mammalian

Mutation

silet mutations in aa 197 - 203 to confer resista…

Promoter

EF1alpha core

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2-7SK-Blast

Plasmid

#183620

Purpose

lentiCRISPRv2 derivative with modified 7SK promoter driving sgRNA and encoding Blasticidin resistance. Compatible with inserts designed for lentiCRISPRv2.

Depositor

Bill Sugden

Article

Hutcheson et al ACS Synth Biol. 2022 Jun 29. doi: 10.102

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Modified 7SK

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO_AU1.HSV.Ollas_mCherry-NLS

Plasmid

#178223

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Article

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

AU1.HSV.Ollas and Nuclear Localization Signal

Promoter

eF1a

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO_AU1.HA.Ollas_mCherry-NLS

Plasmid

#178218

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Article

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

AU1.HA.Ollas and Nuclear Localization Signal

Promoter

eF1a

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO_AU1.C.Ollas_mCherry-NLS

Plasmid

#178212

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Article

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

AU1.C.Ollas and Nuclear Localization Signal

Promoter

eF1a

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pBlastR-MCS-QF-MiniWhite

Plasmid

#165906

Purpose

Blasticidin resistant QF driver vector with Mini-white CDS eye marker. Contains an enhancer grammar GB20 entry point for custom enhancers. Standard cut-and-paste cloning can also be used. Vector uses Purple-White bacteria colony screening for identification of correct cloning

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable Empty Enhancer Driver

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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MS2392.EF1a.SLC6A1.WT.P2A.BLAST.JL.p159

Plasmid

#170170

Purpose

Lentiviral Expression of EF1a.SLC6A1.WT

Depositor

Christopher Moxham

Insert

SLC6A1 (SLC6A1 Human)

Use

Lentiviral

Mutation

n/a

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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MS2392.EF1a.SLC6A1.WT.EGFP.P2A.BLAST.JL.p164

Plasmid

#170169

Purpose

Lentiviral Expression of EF1a.SLC6A1.WT.EGFP

Depositor

Christopher Moxham

Insert

SLC6A1 (SLC6A1 Human)

Use

Lentiviral

Mutation

n/a

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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