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pnEA-NpM-DmThor_50-63-DmeIF4Ga_631-675-GB1_AD

Plasmid

#148515

Purpose

Bacterial Expression of DmThor_50-63-DmeIF4Ga_631-675

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

DmThor_50-63-DmeIF4Ga_631-675 (Thor Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-UbC-rtTA-Ngn2:2A:EGFP

Plasmid

#127288

Purpose

all-in-one inducible LV expression construct for Ngn2:2A:EGFP (aka. UNG; for direct neuronal conversion)

Depositor

Fred Gage

Article

Schafer et al Nat Neurosci. 2019 Feb;22(2):243-255. do

Insert

Ngn2:2A:Ascl1 (NEUROG2 Human)

Use

Lentiviral

Tags

Ngn2 and EGFP linked via 2A

Expression

Mammalian

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCAGGS/XBB.1.5-RBD

Plasmid

#213038

Purpose

Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.XBB.1.5 variant

Depositor

Priyamvada Acharya

Article

Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

Insert

pCAGGS/XBB.1.5-RBD (S )

Tags

6 His Tag

Expression

Mammalian

Mutation

G339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS/EG5-RBD

Plasmid

#213410

Purpose

Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.EG5 variant

Depositor

Priyamvada Acharya

Article

Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

Insert

pCAGGS/EG5-RBD (S )

Tags

6 His Tag

Expression

Mammalian

Mutation

G339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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SpyTag-RBD Omicron XBB.1.5

Plasmid

#214724

Purpose

Expresses SARS-CoV-2 Omicron XBB.1.5 RBD (receptor-binding domain from Spike) with N-terminal SpyTag fusion in mammalian cells

Depositor

Mark Howarth

Article

Hills et al Nat Nanotechnol. 2024 May 6. doi: 10.103

Insert

Receptor-Binding Domain (S SARS-CoV-2)

Tags

SpyTag

Expression

Mammalian

Promoter

CMV

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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GFP Cav2.2 HA D122A pcDNA3

Plasmid

#206120

Purpose

expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and an exofacial double HA tag in domain II and a D122A mutation that disrupts the interaction with ?2?-1

Depositor

Annette Dolphin

Article

Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

Insert

cacna1b (CACNA1B Rabbit)

Expression

Mammalian

Mutation

D122A

Promoter

CMV

Available Since

Nov. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ERBB2-V5/HIS

Plasmid

#201103

Purpose

expression of human ERBB2 receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

human ERBB2 receptor tyrosine kinase, full length, wildtype (ERBB2 Human)

Tags

V5/His

Expression

Mammalian

Promoter

CMV

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-FGF2-K128N

Plasmid

#120284

Purpose

Produce human recombinant FGF2 K128N in DE3 cells

Depositor

Paul Burridge

Article

Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

Insert

Basic fibroblast growth factor 2 (FGF2 Synthetic, Human)

Tags

6x His

Expression

Bacterial

Mutation

Codon optimized for E. Coli production

Promoter

T7 promoter

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pGENE:EFF-1::mCherry::V5

Plasmid

#133020

Purpose

EFF-1 fused to mCherry fluorescent protein cloned into the pGene vector for inducible expression in mammalian cells

Depositor

Benjamin Podbilewicz

Article

Perez-Vargas et al Cell. 2014 Apr 10;157(2):407-419. doi: 1

Insert

EFF-1::mCherry::V5

Tags

V5; mCherry

Expression

Mammalian

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO-mito-tagRFP

Plasmid

#185599

Purpose

Lentiviral construct for expression of tagRFP-T targeted to mitochondria

Depositor

Jarrod Johnson

Article

Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

Insert

mito-tagRFP-T

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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Flag FoxO3

Plasmid

#14937

Depositor

Joan Massague

Article

Seoane et al Cell. 2004 Apr 16. 117(2):211-23.

Insert

FoxO3 (FOXO3 Human)

Tags

Flag

Expression

Mammalian

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only

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pLKO-perox-tagRFP

Plasmid

#185600

Purpose

Lentiviral construct for expression of tagRFP-T targeted to peroxisomes

Depositor

Jarrod Johnson

Article

Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

Insert

perox-tagRFP-T

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV5 HA FoxO1

Plasmid

#14936

Depositor

Joan Massague

Article

Seoane et al Cell. 2004 Apr 16. 117(2):211-23.

Insert

FoxO1 (Foxo1 Mouse)

Tags

HA

Expression

Mammalian

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
Let-7b-GFP

Plasmid

#25404

Depositor

Sean Morrison

Article

Nishino et al Cell. 2008 Oct 17. 135(2):227-39.

Insert

Mirlet7b (Mirlet7b Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

None

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only
pUREF-EX

Plasmid

#199331

Purpose

A pEF-BOS-EX-derived expression vector that contains an SV40 promoter-driven puromycin resistance gene

Depositor

Rikiro Fukunaga

Article

Fujii et al Genes Cells. 2023 Mar 2. doi: 10.1111/gt

Type

Empty backbone

Expression

Mammalian

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMKO.1 GFP B56 gamma3 shRNA 14-1

Plasmid

#10681

Depositor

William Hahn

Article

Chen et al Cancer Cell. 2004 Feb . 5(2):127-36.

Insert

PP2A B56 gamma3 shRNA (PPP2R5C Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
Chx10 3kb AP (CC#318)

Plasmid

#15205

Depositor

Connie Cepko

Article

Rowan et al Dev Biol. 2005 May 15. 281(2):240-55.

Insert

Chx10 3kb AP reporter (Vsx2 Mouse)

Expression

Bacterial

Available Since

June 8, 2007

Availability

Academic Institutions and Nonprofits only
pMKO.1 GFP B56 alpha shRNA 6-3

Plasmid

#10680

Depositor

William Hahn

Article

Chen et al Cancer Cell. 2004 Feb . 5(2):127-36.

Insert

PP2A B56 alpha shRNA (PPP2R5A Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
HA Raptor mutant 1

Plasmid

#8470

Depositor

David Sabatini

Article

Kim et al Cell 2002 Jul 26;110(2):163-75.

Insert

Raptor (RPTOR Human)

Tags

ha

Expression

Mammalian

Mutation

194YDC196-AAA (Y194A, D195A, C196A)

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
HA Raptor mutant 4

Plasmid

#8474

Depositor

David Sabatini

Article

Kim et al Cell 2002 Jul 26;110(2):163-75.

Insert

Raptor (RPTOR Human)

Tags

ha

Expression

Mammalian

Mutation

391SQ392-PA (S391P, Q392A)

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only