We narrowed to 17,102 results for: emb

1. FKBP12-CB2x2-mTagBFP2-HAx2

   Plasmid

   #207146

   Purpose

   Tandem calcium-buffer with rapalog dimerizer

   Depositor

   Antony Galione

   Article

   Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

   Insert

   Calbindin-2 (CALB2 Human)
   

   Tags

   FKBP12, HA, and mTagBFP2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAV10.K5

   Plasmid

   #63210

   Purpose

   pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI or BciVI).

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Available Since

   Feb. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAV10.K4

   Plasmid

   #63212

   Purpose

   pCACS backbone (Amp), TRP1, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into chrIXR: 387328-388330.

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Available Since

   Feb. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCIneo-V5-SBP-HseIF4AII_AC

   Plasmid

   #148439

   Purpose

   Mammalian Expression of HseIF4AII

   Depositor

   Elisa Izaurralde

   Article

   Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

   Insert

   HseIF4AII (EIF4A2 Human)
   

   Expression

   Mammalian

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. MAC_C_ERBB2

   Plasmid

   #187776

   Purpose

   MAC-tagged gene expression of human ERBB2

   Depositor

   Markku Varjosalo

   Article

   Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

   Insert

   ERBB2 (ERBB2 Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5FRT/TO-Strep/HA-UBE2QL1

   Plasmid

   #124665

   Purpose

   Expresses UBE2QL1 with N-terminal Strep-HA tag in mammalian cells

   Depositor

   Hemmo Meyer

   Article

   Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

   Insert

   UBE2QL1 (UBE2QL1 Human)
   

   Tags

   2xStrep/HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEF NS34a-msGFP-'Q-mito

   Plasmid

   #158764

   Purpose

   Expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.

   Depositor

   Edwin Chapman

   Article

   Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

   Insert

   NS3/4a protease and msGFP
   

   Expression

   Mammalian

   Mutation

   Flexible linkers between the protease and msGFP a…

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBTK601

   Plasmid

   #110607

   Purpose

   BTK assembled plasmid - encodes Cas9 with no targeting sgRNA (used with suicide plasmid for broad-host-range genome alteration)

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   Cas9
   

   Use

   Synthetic Biology

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-BICD2N

   Plasmid

   #64070

   Purpose

   N-terminal 400 amino acids of mouse BICD2 codon optimized for Sf9 expression with a N-terminal GFP tag and a TEV-cleavable His-ZZ tag for purification (GFP-BICD2N)

   Depositor

   Andrew Carter

   Article

   Schlager et al EMBO J. 2014 Sep 1;33(17):1855-68. doi:

   Insert

   BICD2N (1-400) (Bicd2 Mouse)
   

   Tags

   GFP and His-ZZ-TCS

   Expression

   Insect

   Mutation

   Codon optimized for Sf9 expression

   Available Since

   Sept. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. POPDC2 pEGFP-N2 (1087)

    Plasmid

    #62030

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

    Insert

    POPDC2 (POPDC2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

11. NKP68 RFP (790)

    Plasmid

    #62003

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al PLoS One. 2011 Apr 14;6(4):e18762. doi:

    Insert

    NKP68 (TMUB1 Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    pgk

    Available Since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

12. Lyn11C3S-EGFP

    Plasmid

    #202747

    Purpose

    weakly plasma membrane targeted fluorescent protein

    Depositor

    Gerry Hammond

    Article

    Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

    Insert

    LYN (1-11) (C3S):EGFP (LYN Human)
    

    Expression

    Mammalian

    Available Since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEM-PH-citrine

    Plasmid

    #131406

    Purpose

    Purpose: synthesis of mRNA encoding a membrane marker. Insert: PH domain of the human phospholipase C delta 1 (PLCD1) fused with the yellow fluorescent protein mCitrine

    Depositor

    Lars Hufnagel

    Article

    Guignard et al Science. 2020 Jul 10;369(6500). pii: 369

    Insert

    PH
    

    Use

    In vitro transcription

    Tags

    citrine yellow fluorescent protein

    Promoter

    T7

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAcGP67A-hBAI3-HormR-GAIN

    Plasmid

    #37848

    Depositor

    Axel Brunger

    Article

    Arac et al EMBO J. 2012 Feb 14;31(6):1364-78. doi:

    Insert

    BAI3 (BAI3 Human)
    

    Use

    Baculovirus transfer vectors

    Tags

    8xHIS

    Mutation

    HormR and GAIN domains only (aa498–868

    Promoter

    Polyhedrin

    Available Since

    July 6, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pTK165_Mem-mCherry-4.1G-CTD

    Plasmid

    #46362

    Depositor

    Iain Cheeseman

    Article

    Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

    Insert

    4.1G C-terminal domain (EPB41L2 Human)
    

    Tags

    Mem (N-terminal 20 amino acids of neuromodulin fo…

    Expression

    Mammalian

    Mutation

    C-terminal domain from 886-1005 amino acids

    Promoter

    CMV

    Available Since

    Aug. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5FRT/TO-Strep/HA-UBE2Q2

    Plasmid

    #124668

    Purpose

    Expresses UBE2Q2 with N-terminal Strep-HA tag in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

    Insert

    UBE2Q2 (UBE2Q2 Human)
    

    Tags

    2xStrep/HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEMHE:hTRAAK(Q258K)

    Plasmid

    #133077

    Purpose

    X. laevis expression vector. It will generate the human TRAAK channel

    Depositor

    Dan Minor

    Article

    Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

    Insert

    Potassium channel subfamily K member 2 (KCNK2, TREK-1), Q258K (Kcnk2 Mouse)
    

    Mutation

    Q258K

    Promoter

    T7

    Available Since

    Oct. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hSyn-mCherry-T2A-miniSOG-C-Far

    Plasmid

    #193915

    Purpose

    Expresses miniSOG targeted to the plasma membrane of neurons, joined by a T2A cleavage site with mCherry; for AAV production

    Depositor

    Xiao Wang

    Article

    Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

    Insert

    miniSOG
    

    Use

    AAV

    Tags

    Farnesylation signal sequence and Flag tag

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMM2-rPMCA3b

    Plasmid

    #47763

    Purpose

    mammalian expression of rat PMCA3b

    Depositor

    John Penniston, Emanuel Strehler

    Article

    Enyedi et al J Biol Chem. 1997 Oct 31;272(44):27525-8

    Insert

    PMCA3b (Atp2b3 Rat)
    

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Sept. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pCDH-AtAFB2-mTagBFP2

    Plasmid

    #179889

    Purpose

    Expresses AtAFB2 (auxin-inducible degron system) fused to mTagBFP2 (lentiviral plasmid)

    Depositor

    Patrick Caswell

    Article

    Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

    Insert

    AtAFB2 (AFB2 Mustard Weed, Synthetic)
    

    Use

    Lentiviral

    Tags

    mTagBFP2

    Expression

    Bacterial and Mammalian

    Promoter

    EF1

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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