We narrowed to 11,615 results for: SHA

1. pLenti-Blast-sgNT

   Plasmid

   #138678

   Purpose

   Expresses a Non-targeting sgRNA and Cas9

   Depositor

   Reuben Shaw

   Article

   Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

   Insert

   sgNT
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEG302_dCAS9-MQ1(Q147L)_g4+g10+g18

   Plasmid

   #172316

   Purpose

   CRISPR dCas9 directly fused to a variant of bacterial DNA methyltransferase MQ1 to target CG specific methylation to the FWA locus with three guide RNAs

   Depositor

   Steven Jacobsen

   Article

   Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

   Insert

   g18_U6_g10_U6_g4_U6_UBQ10_Ω_dCas9_MQ1(Q147L)_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA Flag Yap1 Y357F

   Plasmid

   #18882

   Depositor

   Yosef Shaul

   Article

   Levy et al Mol Cell. 2008 Feb 15. 29(3):350-61.

   Insert

   Yap1 Y357F (YAP1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Y357F

   Promoter

   Available since

   Aug. 14, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX-4T-1 3xFlag-STAT1

   Plasmid

   #82722

   Purpose

   Expresses human STAT1

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   STAT1 (STAT1 Human)
   

   Use

   Tags

   3xFLAG and GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

5. pJS145

   Plasmid

   #179159

   Purpose

   Encodes 2xNLS::GFP(flexon), codon optimized for C. elegans. Blocks expression from ubiquitous promoter rps-27p with Flexon. Excision of flexon with Cre allows for strong expression.

   Depositor

   Iva Greenwald

   Article

   Shaffer et al Proc Natl Acad Sci U S A. 2022 Jan 18;11

   Insert

   rps-27p::2xNLS::GFP(flexon)::unc-54 3' UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Flexon replacing 2nd intron of 2xNLS::GFP

   Promoter

   rps-27p

   Available since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV2/9-CW3SL-mCherry-DrTrkB

   Plasmid

   #184002

   Purpose

   AAV production for expression of optically controlled eDrTrkB with N-terminal fusion of mCherry

   Depositor

   Vladislav Verkhusha, Dmitry Gerashchenko

   Article

   Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

   Insert

   eDrTrkB (NTRK2 Human)
   

   Use

   AAV

   Tags

   Ig kappa secretory signal, mCherry, Deinococcus r…

   Expression

   Mutation

   Promoter

   Available since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLX302-MKP5-V5 puro

   Plasmid

   #87770

   Purpose

   Expresses V5 tagged human MAPK phosphatase 5 (MKP5/DUSP10)

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   MKP5 (DUSP10 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CaMKII-iGECI

   Plasmid

   #160423

   Purpose

   AAV plasmid for CaMKII-driven expression of a genetically-encoded calcium indicator iGECI

   Depositor

   Vladislav Verkhusha

   Article

   Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

   Insert

   iGECI
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKII

   Available since

   Nov. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAGH10 - RlucFluc minus 1 HIV-1

   Plasmid

   #198224

   Purpose

   Fluorescence reporters for -1 ribosomal frameshifting in mammalian cells

   Depositor

   Isha Jain

   Article

   Baik et al Mol Cell. 2023 Mar 16;83(6):942-960.e9.

   Inserts

   Renilla luciferase
   Firefly luciferase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   March 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. EF1a_PAX7_P2A_Hygro_Barcode

    Plasmid

    #120472

    Purpose

    Barcoded lentiviral vector to express PAX7 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    PAX7 (PAX7 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCEP4-myc-HLA-B8

    Plasmid

    #135512

    Purpose

    Mammalian expression of myc-tagged HLA-B*08:01

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-B*08:01 (HLA-B Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. EF1a_SOX3_P2A_Hygro_Barcode

    Plasmid

    #120481

    Purpose

    Barcoded lentiviral vector to express SOX3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    SOX3 (SOX3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLX302-JSP1-V5 puro

    Plasmid

    #87773

    Purpose

    Expresses V5 tagged human JSP1 (DUSP22)

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    JSP1 (DUSP22 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. EF1a_ONECUT1_P2A_Hygro_Barcode

    Plasmid

    #120470

    Purpose

    Barcoded lentiviral vector to express ONECUT1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    ONECUT1 (ONECUT1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSLIK TT FLAG-HA RBFOX2

    Plasmid

    #59769

    Purpose

    doxycycline-inducible expression of FLAG-HA-tagged human RBFOX2 in mammalian cells

    Depositor

    Phil Sharp

    Article

    Jangi et al Genes Dev. 2014 Mar 15;28(6):637-51. doi

    Insert

    RBFOX2 (RBFOX2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    TRE tight

    Available since

    Oct. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCEP4-myc-H2-Dd

    Plasmid

    #135518

    Purpose

    Mammalian expression of myc-tagged H2-Dd

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    H2-Dd (H2-D1 Mouse)
    

    Use

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    C121S

    Promoter

    CMV

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSECC-sgTom

    Plasmid

    #138661

    Purpose

    Expresses a Tomato-targeting sgRNA, Cas9, and Cre-recombinase

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgTomato
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. EF1a_MYOG_P2A_Hygro_Barcode

    Plasmid

    #120465

    Purpose

    Barcoded lentiviral vector to express MYOG in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    MYOG (MYOG Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEF1a-2xSV40_NLS-NLuc

    Plasmid

    #135953

    Purpose

    Expresses the NanoLuciferase targeted to the nucleus in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    NanoLuciferase
    

    Use

    Tags

    2xSV40-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pVimentin-miRFP703

    Plasmid

    #79996

    Purpose

    Vimentin labeling with near-infrared fluorescent protein miRFP703

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

    Insert

    Vimentin (VIM Human)
    

    Use

    Tags

    miRFP703

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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