161,273 results

1. IDP432

   Plasmid

   #87000

   Purpose

   plasmid for cell culture expression of SYNseq components

   Depositor

   Anthony Zador

   Article

   Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

   Insert

   SNAP-Nlg1AB-1xλN(i)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. Tcf/Lef:H2B-GFP

   Plasmid

   #32610

   Depositor

   Anna-Katerina Hadjantonakis

   Article

   Ferrer-Vaquer et al BMC Dev Biol. 2010 Dec 21;10:121.

   Insert

   Tcf/Lef:H2B-GFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   hsp68

   Available since

   July 12, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-CMV-LoxP-DsRed-LoxP-eGFP

   Plasmid

   #65726

   Purpose

   Functions as a genetic Cre reporter that switches from DsRed expression to eGFP expression upon presence of Cre recombinase.

   Depositor

   Jacco van Rheenen

   Article

   Zomer et al Cell. 2015 May 21;161(5):1046-57. doi: 1

   Insert

   LoxP-DsRed-LoxP-eGFP
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pD3sE_SC12

   Plasmid

   #219720

   Purpose

   DV3 E dimer with fusion loop mutation and increased expression

   Depositor

   Brian Kuhlman

   Article

   Phan et al J Biol Chem. 2022 Jul;298(7):102079. doi

   Insert

   DV3 sE SC12
   

   Use

   Tags

   8xHis and human serum albumin (HSA)

   Expression

   Mammalian

   Mutation

   G106D, A257W, T260R, F277W, A278P, G29K, T33V, A3…

   Promoter

   Available since

   May 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSFFV_mNG2(11)1-10

   Plasmid

   #82610

   Purpose

   Lentiviral vector of constitutive expression of mNeonGreen2(1-10)

   Depositor

   Bo Huang

   Article

   Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

   Insert

   mNeonGreen2(1-10)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   K128M, S142T, R150M, G172V and K213M

   Promoter

   SFFV

   Available since

   May 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSUMO His6 SUMO MYC3

   Plasmid

   #177848

   Purpose

   Bacterial Expression of MYC3

   Depositor

   Karsten Melcher

   Article

   Zhang et al Nature. 2015 Sep 10;525(7568):269-73. do

   Insert

   MYC3 (MYC3 Mustard Weed)
   

   Use

   Tags

   6xHis-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. 7TFP CDH1 mutant reporter

   Plasmid

   #91703

   Purpose

   lentiviral luciferase reporter containing CDH1 promoter with mutant E-boxes

   Depositor

   Bob Weinberg

   Article

   Pattabiraman et al Science. 2016 Mar 4;351(6277):aad3680. d

   Insert

   CDH1 mutant promoter (CDH1 Human)
   

   Use

   Lentiviral and Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   mutant E-boxes

   Promoter

   CDH1 mutant

   Available since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET29b-H6_Streptavidin_sfGFP

   Plasmid

   #124296

   Purpose

   His6-tagged Streptavidin GFP fusion protein

   Depositor

   Mark Arbing

   Insert

   His6 tag - TEV protease site - T7 tag - Streptavidin (15-159) - superfolder GFP
   

   Use

   Tags

   His6 and T7 tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.CAG.(mito).iATPSnFR2.A95A.A119L.HaloTag

   Plasmid

   #209722

   Purpose

   Expresses mitochondrially targeted low affinity ratiometric ATP sensor

   Depositor

   Timothy Ryan

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   (mito).iATPSnFR2.A95A.A119L.HaloTag
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTAHR TH-p2a-TD:Tomato (floxed selection Puro)

    Plasmid

    #135814

    Purpose

    Fluorescent reporter for knock-in of TdTomato into the TH locus

    Depositor

    Tim Ahfeldt, Lee Rubin

    Article

    Ahfeldt et al Stem Cell Reports. 2019 Dec 20. pii: S22

    Insert

    TH-p2a-TD:Tomato (red fluorophore protein) construct as a reporter for TH expression. (TH Human)
    

    Use

    Tags

    p2a-TdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. sfGFP-C1

    Plasmid

    #54579

    Purpose

    Localization: C1 Cloning Vector, Excitation: 485, Emission: 507

    Depositor

    Michael Davidson, Geoffrey Waldo

    Article

    Pédelacq et al Nat Biotechnol. 2006 Jan;24(1):79-88. do

    Type

    Empty backbone

    Use

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. TCARZ-SV40-GFP-PAmin-GF3-R4A22

    Plasmid

    #203769

    Purpose

    For production of template RNA for use in PRINT, GFP under SV40 promoter

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    PRINT template RNA for GFP
    

    Use

    Ivt template

    Tags

    Expression

    Mutation

    Promoter

    SV40

    Available since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBAD-Citron1

    Plasmid

    #134300

    Purpose

    Bacterial expression of direct-response citrate sensor

    Depositor

    Robert Campbell

    Article

    Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

    Insert

    Citron1
    

    Use

    Tags

    6-His tag

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    April 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR223_SDHA_WT

    Plasmid

    #82278

    Purpose

    Gateway Donor vector containing SDHA, part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, William Hahn, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    SDHA (SDHA Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV/SB11

    Plasmid

    #26552

    Depositor

    Perry Hackett

    Article

    Ivics et al Cell. 1997 Nov 14. 91(4):501-10.

    Insert

    Sleeping Beauty
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Methionine 243 to Glutamine Threonine 136 to Arg…

    Promoter

    Available since

    Oct. 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENTR hH1-TERRA

    Plasmid

    #111381

    Purpose

    TERRA with approximately 130 telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences under control of hH1 promoter and terminator

    Depositor

    Klaus Holzmann

    Article

    Kreilmeier et al Genes (Basel). 2016 Aug 16;7(8). pii: ge

    Insert

    hH1-TERRA
    

    Use

    Gateway entry cloning

    Tags

    Expression

    Mutation

    Promoter

    hH1

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBAtC

    Plasmid

    #78097

    Purpose

    Plant expression of Cas9 and gRNA empty backbone; Basta resistance

    Depositor

    Jin-Soo Kim

    Article

    Kim et al J Integr Plant Biol. 2016 Mar 4. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    NLS-HA

    Expression

    Plant

    Mutation

    Promoter

    U6

    Available since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. HA-HIF1alpha-pcDNA3

    Plasmid

    #18949

    Depositor

    William Kaelin

    Article

    Kondo et al Cancer Cell. 2002 Apr . 1(3):237-46.

    Insert

    Hypoxia inducible factor 1 alpha (HIF1A Human)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    wt cDNA

    Promoter

    Available since

    Sept. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti UBC MitoFlux (pSu9-HaloTag7-mGFP-STOP)

    Plasmid

    #215010

    Purpose

    Express a Mitophagy reporter in mammalian cells (untested)

    Depositor

    Wade Harper

    Insert

    pSu9 (Presequence of Fo-ATPase subunit 9)
    

    Use

    Lentiviral

    Tags

    HaloTag(7)-mGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pME-Actin-Vhh-sfGFP-P2A-1xHA-tdiRFP-caax (JDW 1311)

    Plasmid

    #224497

    Purpose

    Gateway compatible middle entry clone containing an Actin nanobody fused to sfGFP followed by P2A and tdiRFP-caax tag (GFP actin reporter and cell membrane iRFP reporter)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Actin-Vhh-sfGFP-P2A-HA-tdiRFP-caax
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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