176,436 results

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pAAV-mTH-GFP

Plasmid

#99128

Purpose

An AAV genome that expresses GFP from the mouse TH promoter

Depositor

Viviana Gradinaru

Publication

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

GFP

Use

AAV

Expression

Mammalian

Promoter

mTH

Available since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAnc80L65AAP

Plasmid

#92307

Purpose

Adeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep expression construct with endogenous AAP

Depositor

Luk Vandenberghe

Publication

Landegger et al Nat Biotechnol. 2017 Mar;35(3):280-284.

Insert

Ancestral AAV Capsid Anc80L65AAP

Use

AAV and Synthetic Biology

Expression

Mammalian

Promoter

Rep2

Available since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pTRIP-CMV-tagRFP-FLAG-cGAS

Plasmid

#86676

Purpose

Expresses catalytically-active tagRFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.

Depositor

Nicolas Manel

Publication

Raab et al Science. 2016 Apr 15;352(6283):359-62. d

Insert

tagRFP-FLAG-cGAS

Use

Lentiviral

Available since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA-RLuc8

Plasmid

#87121

Purpose

Mammalian expression vector expressing mutant version of the reporter gene-Renilla Luciferase (Rluc8), which is more stable in mouse serum and has more light output.

Depositor

Sanjiv Sam Gambhir

Publication

Loening et al Protein Eng Des Sel. 2006 Sep;19(9):391-

Insert

Renilla Luciferase

Use

Luciferase

Expression

Mammalian

Mutation

A55T, C124A, S130A, K136R, A143M, M185V, M253L, a…

Promoter

CMV

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pAAVS1-PDi-CRISPRn

Plasmid

#73500

Purpose

Dox-inducible CRISPR nuclease (CRISPRn) knock in construct into the AAVS1 locus

Depositor

Bruce Conklin

Publication

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Inserts

Cas9
rtTA

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Promoter

CAG and TRE

Available since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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Fuw-dCas9-Dnmt3a_IM

Plasmid

#84478

Purpose

Lentiviral construct to express dCas9 fused with an inactive Dnmt3a catalytic domain (E664A, E756A)

Depositor

Rudolf Jaenisch

Publication

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

dCas9-Dnmt3a_IM

Use

Lentiviral

Expression

Mammalian

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET30-2-GAPDH

Plasmid

#83910

Purpose

stable overexpression

Depositor

David Sabatini

Publication

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

glyceraldehyde-3-phosphate dehydrogenase (GAPDH Human)

Tags

6x His tags

Expression

Bacterial

Promoter

T7

Available since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pMAL-his-LbCpf1-EC

Plasmid

#79008

Purpose

Bacterial expression - MBP-his tagged LbCpf1 (E.coli codon optimized)

Depositor

Jin-Soo Kim

Publication

Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

Insert

LbCpf1

Tags

MBP-6xHis-NLS

Expression

Bacterial

Promoter

tac promoter

Available since

July 1, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pQCXIP-BSR-GFP11

Plasmid

#68716

Purpose

Split GFP assay

Depositor

Yutaka Hata

Publication

Kodaka et al Exp Cell Res. 2015 Aug 15;336(2):171-81.

Insert

bsr

Use

Retroviral

Tags

FLAG and GFP11

Expression

Mammalian

Available since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

22 Articles

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pDD287 (mKate2^SEC^3xMyc

Plasmid

#70685

Purpose

mKate2^SEC^3xMyc vector with ccdB sites for cloning homology arms

Depositor

Bob Goldstein

Publication

Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

Insert

mKate2-C1^SEC^3xFlag

Use

CRISPR and Cre/Lox

Tags

3xMyc and C. elegans codon-optimized mKate2

Expression

Worm

Available since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pBeloBac11loxP2272

Plasmid

#60342

Purpose

This is a BAC vector that contains both wt and mutant loxP sites.

Depositor

Jon Coren

Publication

Coren et al BMC Res Notes. 2017 Jul 28;10(1):344. do

Type

Empty backbone

Use

Cre/Lox

Expression

Bacterial

Available since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.1_pMagFast2(3x)-iRFP

Plasmid

#67297

Purpose

Blue light dependent photoswitching system

Depositor

Moritoshi Sato

Publication

Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

Insert

pMagFast2(3x)-iRFP

Expression

Mammalian

Promoter

CMV

Available since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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ptf-Galectin3

Plasmid

#64149

Purpose

Expression in mammalian cells

Depositor

Tamotsu Yoshimori

Publication

Maejima et al EMBO J. 2013 Aug 28;32(17):2336-47. doi:

Insert

Galectin3 (LGALS3 Human)

Tags

EGFP and mRFP

Expression

Mammalian

Promoter

CMV

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pCRII_TOPO_hNEAT1

Plasmid

#61518

Purpose

human NEAT1_v1 short isoform 3.7kb insert in general cloning vector

Depositor

Archa Fox

Publication

Clemson et al Mol Cell. 2009 Mar 27;33(6):717-26. doi:

Insert

NEAT1 (NEAT1 Human)

Use

General cloning vector

Mutation

*see comment below

Available since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pAAV-Ef1a-fDIO EYFP

Plasmid

#55641

Purpose

Flp-Dependent EYFP

Depositor

Karl Deisseroth

Publication

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Has service

AAV1, AAV2, AAV5, AAV8, AAV9, and AAV Retrograde

Insert

EYFP

Use

AAV

Expression

Mammalian

Promoter

Ef1a

Available since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

31 Articles

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pAAV-CaMKIIa-EGFP

Plasmid

#50469

Purpose

EGFP under the control of CaMKIIa promoter

Depositor

Bryan Roth

Has service

AAV1, AAV2, AAV5, AAV8, AAV9, AAV PHP.eB, and AAV Retrograde

Insert

EGFP

Use

AAV

Tags

N/A

Promoter

CaMKIIa

Available since

March 17, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

74 Articles

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FLAG-Stx17

Plasmid

#45911

Depositor

Noboru Mizushima

Publication

Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

Insert

Syntaxin 17 (STX17 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pcDNA3.1-SC35-cMyc

Plasmid

#44721

Depositor

Kathleen Scotto

Publication

Shi et al Mol Cell Biol. 2008 Jan;28(2):883-95. Ep

Insert

SC35 (SRSF2 Human)

Tags

his and myc

Expression

Mammalian

Promoter

CMV

Available since

April 29, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pEGFP-N1-RACK1

Plasmid

#41088

Depositor

Anna Huttenlocher

Publication

Cox et al Mol Biol Cell. 2003 Feb;14(2):658-69.

Insert

Receptor for activated C kinase (RACK1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pcDNA-D3cpv

Plasmid

#36323

Depositor

Amy Palmer, Roger Tsien

Publication

Palmer et al Chem Biol. 2006 May;13(5):521-30.

Insert

D3cpv second generation cameleon (calcium sensor)

Expression

Mammalian

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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