We narrowed to 5,803 results for: SUP

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Frt-hspB2

Plasmid

#63093

Purpose

Expression of HSPB2 (small HSP) in mammalian cells

Depositor

Harm Kampinga

Article

Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

Insert

hspb2 (HSPB2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA4TO-3XFLAG Halo delta 1-117 nsp1

Plasmid

#175423

Purpose

Expresses delta 1-117 CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

CoV2 delta 1-117 nsp1

Tags

3XFLAG Halo

Expression

Mammalian

Mutation

nsp1 C-terminus

Promoter

CMV

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA4 3xFLAG Halo delta 118-180 nsp1

Plasmid

#175424

Purpose

Expresses delta 118-180 CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

CoV2 delta 118-180 nsp1

Tags

3XFLAG Halo

Expression

Mammalian

Mutation

N-terminus of nsp1

Promoter

CMV

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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MSCV-TAP(FLAG-HA) CYLD

Plasmid

#60031

Purpose

CYLD with N-terminal TAP-tag (HA,FLAG) and IRES-Puro to allow tagged protein to be maintained

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

Insert

Cylindromatosis (CYLD Human)

Use

Retroviral

Tags

FLAG, HA, and IRES Puro

Expression

Mammalian

Promoter

LTR-driven expression

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
pDN-T1TCbt

Plasmid

#44514

Depositor

Gabor Balazsi

Article

Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

Inserts

PGal1-T123 promoter (GAL1 Synthetic, Budding Yeast)
tetR::ymcherry

Use

Synthetic Biology; Expression regulator/reporter

Expression

Yeast

Mutation

Three tandem tet operators (3XtetO2) downstream o…

Promoter

PGal1-T123 promoter

Available Since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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pDEST-FRT/T0-GFP-BRCA1 1364-1437

Plasmid

#71119

Purpose

Mammalian expression of GFP-BRCA1 1364-1437

Depositor

Daniel Durocher

Article

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

BRCA1 (BRCA1 Human)

Tags

GFP

Expression

Mammalian

Mutation

contains amino acids 1364-1437 of BRCA1

Promoter

CMV/TO

Available Since

Dec. 9, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pDN-T1GZmbh

Plasmid

#44522

Depositor

Gabor Balazsi

Article

Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

Inserts

PGal1-T123 promoter (GAL1 Synthetic, Budding Yeast)
yEGFP::ZeoR

Use

Synthetic Biology; Expression regulator/reporter

Expression

Yeast

Mutation

Three tandem tet operators (3XtetO2) downstream o…

Promoter

PGal1-T123 promoter

Available Since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNcor1#2/Cre

Plasmid

#173606

Purpose

Expresses a Ncor1-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Ncor1 (Ncor1 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA4TO-3XFLAG Halo R124A/K125A nsp1

Plasmid

#175417

Purpose

Expresses R124A/K125A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

CoV2 R124A/K125A nsp1

Tags

3XFLAG Halo

Expression

Mammalian

Mutation

R124A and K125A

Promoter

CMV

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pRV-GN

Plasmid

#106373

Purpose

creating stable line with GFP and G418 resistance

Depositor

Tong-Chuan He

Article

Liao et al Gene Ther. 2017 Jun;24(6):333-341. doi:

Insert

eGFP and geneticin/G418 resistance gene

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pDN-G1TCbt

Plasmid

#44515

Depositor

Gabor Balazsi

Article

Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

Inserts

PGal1-D12 promoter (GAL1 Synthetic, Budding Yeast)
tetR::ymcherry

Use

Synthetic Biology; Expression regulator/reporter

Expression

Yeast

Mutation

Two tandem tet operators (2XtetO2) downstream of …

Promoter

PGal1-D12 promoter

Available Since

April 30, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-3XHA-Zmat3R99Q

Plasmid

#167344

Purpose

Expresses HA-tagged R99Q mutant Zmat3

Depositor

Laura Attardi

Article

Bieging-Rolett et al Mol Cell. 2020 Nov 5;80(3):452-469.e9. d

Insert

Zinc finger matrin-type 3 (Zmat3 Mouse)

Tags

3HA

Expression

Mammalian

Mutation

changed arginine 99 to glutamine

Promoter

CMV

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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sgMAPKAP1_2

Plasmid

#124911

Purpose

Targeting human MAPKAP1 (SIN1) gene by CRISPR-Cas9

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MAPKAP1 (MAPKAP1 Human)

Use

CRISPR and Lentiviral

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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sgMAPKAP1_3

Plasmid

#124913

Purpose

Targeting human MAPKAP1 (SIN1) gene by CRISPR-Cas9

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MAPKAP1 (MAPKAP1 Human)

Use

CRISPR and Lentiviral

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pN1 VAMP2 R125TAG

Plasmid

#69877

Purpose

express in mammalian cells for unnatural amino acid incorporation into VAMP2

Depositor

Silvio Rizzoli

Article

Vreja et al ACS Nano. 2015 Nov 24;9(11):11034-41. do

Insert

VAMP2 (Vamp2 Rat)

Use

Synthetic Biology

Expression

Mammalian

Mutation

Amber and Ochre stop codons in the former linker …

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only
Lenti-sgNf2#1/Cre

Plasmid

#173643

Purpose

Expresses a Nf2-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Nf2 (Nf2 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pDN-G1GZmbh

Plasmid

#44516

Depositor

Gabor Balazsi

Article

Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

Inserts

PGal1-D12 promoter (GAL1 Synthetic, Budding Yeast)
yEGFP::ZeoR

Use

Synthetic Biology; Expression regulator/reporter

Expression

Yeast

Mutation

Two tandem tet operators (2XtetO2) downstream of …

Promoter

PGal1-D12 promoter

Available Since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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Lenti-sgSmad4#1/Cre

Plasmid

#173617

Purpose

Expresses a Smad4-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Smad4 (Smad4 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pMIR-Report-SPRED1-3'UTR (WT - miR-206 site)

Plasmid

#62577

Purpose

Translational Luciferase Reporter containing a fragment of the 3'UTR of SPRED1 containing a miR-206 binding site

Depositor

Michael Ruppert

Article

Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

Insert

SPRED1 (SPRED1 Human)

Use

Luciferase

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
pRK5-FLAG-FLCN (C terminus)

Plasmid

#72296

Purpose

overexpression

Depositor

David Sabatini

Article

Tsun et al Mol Cell. 2013 Nov 21;52(4):495-505. doi

Insert

FLCN C-terminus (FLCN Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains only C terminus of FLCN as defined in PM…

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
Lenti-sgZmat3.55.9/Cre

Plasmid

#167853

Purpose

Expresses Cre recombinase and an sgRNA targeting Zmat3

Depositor

Laura Attardi

Article

Bieging-Rolett et al Mol Cell. 2020 Nov 5;80(3):452-469.e9. d

Insert

sgRNA targeting Zmat3

Use

CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting

Expression

Mammalian

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO/SH/GW-Mst2

Plasmid

#84300

Purpose

Expresses a tetracycline-inducible SH-tagged (streptavidin-binding peptide and HA tag) version of Mst2. Used in conjunction with Flp-In cells to generate single copy transgene.

Depositor

Jonathan Chernoff

Article

Rawat et al J Biol Chem. 2013 Mar 22;288(12):8762-71

Insert

Mst2 (STK3 Human)

Tags

HA, SBP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pMCB320-sgZmat3.51.5

Plasmid

#169024

Purpose

Expresses an sgRNA targeting Zmat3

Depositor

Laura Attardi

Article

Bieging-Rolett et al Mol Cell. 2020 Nov 5;80(3):452-469.e9. d

Insert

sgRNA targeting Zmat3, mCherry, Puromycin resistance

Use

CRISPR, Lentiviral, and Mouse Targeting

Expression

Mammalian

Promoter

mU6

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pMCB320-sgZmat3.55.9

Plasmid

#169026

Purpose

Expresses an sgRNA targeting Zmat3

Depositor

Laura Attardi

Article

Bieging-Rolett et al Mol Cell. 2020 Nov 5;80(3):452-469.e9. d

Insert

sgRNA targeting Zmat3, mCherry, Puromycin resistance

Use

CRISPR, Lentiviral, and Mouse Targeting

Expression

Mammalian

Promoter

mU6

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-hDIXDC1 S592A

Plasmid

#61223

Purpose

Mammalian expression vector encoding long isoform of human DIXDC1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

human DIXDC1 (DIXDC1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Changed Serine 592 to Alanine

Promoter

CMV

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
Frt-V5-hspB3

Plasmid

#63104

Purpose

Expression of HSPB3 (small HSP) tagged with V5 in mammalian cells

Depositor

Harm Kampinga

Article

Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

Insert

hspb3 (HSPB3 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-Puro-sgSIK3-A

Plasmid

#138682

Purpose

Expresses a mouse SIK3-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK3 mouse (Sik3 Mouse)

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-Puro-sgSIK3-C

Plasmid

#138683

Purpose

Expresses a mouse SIK3-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK3 mouse (Sik3 Mouse)

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Luc-Hdm4-3'UTR-F2d5

Plasmid

#64018

Purpose

Luciferase reporter of human Hdm4 3'UTR F2 fragment mutant #5

Depositor

Lin He

Article

Okada et al Genes Dev. 2014 Mar 1;28(5):438-50. doi:

Insert

HDMX (MDM4 Human)

Expression

Mammalian

Available Since

June 24, 2015

Availability

Academic Institutions and Nonprofits only

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NC1 pGLUE WTX (1-804)

Plasmid

#36956

Depositor

Randall Moon

Article

Major et al Science. 2007 May 18;316(5827):1043-6.

Insert

WTX (AMER1 Human)

Tags

CBP tag, HA, and streptag

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2012

Availability

Academic Institutions and Nonprofits only