We narrowed to 18,106 results for: URE

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delta DE OCT4-GFP-2A-PURO transgenic reporter

Plasmid

#52381

Purpose

BAC recombineering generated construct, used as a reporter for OCT4 expression

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Inserts

GFP-2A-puro
OCT4 (POU5F1 Human)

Expression

Mammalian

Mutation

deleted -3194 - -1768 upstream from OCT4 ATG

Promoter

OCT4

Available Since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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pDONR223-BUB1B

Plasmid

#23858

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

BUB1B (BUB1B Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-NEK2

Plasmid

#23658

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NEK2 (NEK2 Human)

Use

Gateway donor vector

Mutation

Insert encodes truncated protein relative to NP_0…

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-CDK7

Plasmid

#23786

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CDK7 (CDK7 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pSLIK-NFLAG-hEWSR1G511A-CMyc

Plasmid

#50734

Purpose

Produces lentivirus expressing human EWSR1G511A mutant with FLAG tag at its N-terminus and Myc tag at its C-terminus by co-transfection with psPAX2 and pMD2G

Depositor

Zissimos Mourelatos

Article

Couthouis et al Hum Mol Genet. 2012 Jul 1;21(13):2899-91

Insert

EWSR1 G511A (EWSR1 Human)

Use

Lentiviral

Tags

FLAG and Myc

Mutation

Changed Gly 511 to Ala

Promoter

TRE

Available Since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pDONR223-CALM1

Plasmid

#23768

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CALM1 (CALM1 Human)

Use

Gateway donor vector

Mutation

M74T V144E

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-MAPK1

Plasmid

#23498

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAPK1 (MAPK1 Human)

Use

Gateway donor vector

Mutation

missing first codon

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-CARD11

Plasmid

#23482

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CARD11 (CARD11 Human)

Use

Gateway donor vector

Mutation

A291C,C292T,1MM

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pGL4.23-IL2RA CaRE4 (plus strand)

Plasmid

#91850

Purpose

Luciferase reporter for IL2RA enhancer (IGI-P0345)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

IL2RA CaRE4 (plus strand) (IL2RA Human)

Use

Luciferase

Expression

Mammalian

Mutation

Perfect match to reference sequence

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223-AKT1

Plasmid

#23752

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

AKT1 (AKT1 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-CALM2

Plasmid

#23734

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CALM2 (CALM2 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pGL4.23-IL2RA CaRE4 scramble (plus strand)

Plasmid

#91852

Purpose

Luciferase reporter for IL2RA enhancer (IGI-P0347)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

IL2RA CaRE4 scramble (plus strand) (IL2RA Human)

Use

Luciferase

Expression

Mammalian

Mutation

Sequence scrambled from ch10:6094588-6094934

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.23-IL2RA CaRE4 (minus strand)

Plasmid

#91849

Purpose

Luciferase reporter for IL2RA enhancer (IGI-P0626)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

IL2RA CaRE4 (minus strand) (IL2RA Human)

Use

Luciferase

Expression

Mammalian

Mutation

Perfect match to reference sequence

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223-ZAK

Plasmid

#23709

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ZAK (MAP3K20 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pGL4.23-IL2RA CaRE1 (plus strand)

Plasmid

#91837

Purpose

Luciferase reporter for IL2RA enhancer (IGI-P0614)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

IL2RA CaRE1 (plus strand) (IL2RA Human)

Use

Luciferase

Expression

Mammalian

Mutation

Perfect match to reference sequence

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pSLIK-NFLAG-hEWSR1WT-CMyc

Plasmid

#50733

Purpose

Produces lentivirus expressing human EWSR1 wild type with FLAG tag at its N-terminus and Myc tag at its C-terminus by co-transfection with psPAX2 and pMD2G

Depositor

Zissimos Mourelatos

Article

Couthouis et al Hum Mol Genet. 2012 Jul 1;21(13):2899-91

Insert

EWSR1 (EWSR1 Human)

Use

Lentiviral

Tags

FLAG and Myc

Promoter

TRE

Available Since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only
pDONR223-DLG3

Plasmid

#23451

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

DLG3 (DLG3 Human)

Use

Gateway donor vector

Mutation

G381A,G629T

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PRKR

Plasmid

#23452

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKR (EIF2AK2 Human)

Use

Gateway donor vector

Mutation

aa494-511 missing

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pSLIK-NFLAG-hEWSR1P552L-CMyc

Plasmid

#50736

Purpose

Produces lentivirus expressing human EWSR1P552L mutant with FLAG tag at its N-terminus and Myc tag at its C-terminus by co-transfection with psPAX2 and pMD2G

Depositor

Zissimos Mourelatos

Article

Couthouis et al Hum Mol Genet. 2012 Jul 1;21(13):2899-91

Insert

EWSR1 P552L (EWSR1 Human)

Use

Lentiviral

Tags

FLAG and Myc

Mutation

Changed Pro 552 to Leu

Promoter

TRE

Available Since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only
pDONR223-AKT3

Plasmid

#23539

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

AKT3 (AKT3 Human)

Use

Gateway donor vector

Mutation

S2R

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-MAP3K8

Plasmid

#23538

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAP3K8 (MAP3K8 Human)

Use

Gateway donor vector

Mutation

1MM

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pGL4.23-IL2RA CaRE5 (plus strand)

Plasmid

#91841

Purpose

Luciferase reporter for IL2RA enhancer (IGI-P0618)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

IL2RA CaRE5 (plus strand) (IL2RA Human)

Use

Luciferase

Expression

Mammalian

Mutation

Perfect match to reference sequence

Available Since

June 8, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.23-IL2RA CaRE3 (minus strand)

Plasmid

#91835

Purpose

Luciferase reporter for IL2RA enhancer (IGI-P0612)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

IL2RA CaRE3 (minus strand) (IL2RA Human)

Use

Luciferase

Expression

Mammalian

Mutation

rs7893324 A>G, rs7090530 G>T, rs7090512 A&g…

Available Since

June 8, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.23-IL2RA CaRE4 SNP (plus strand)

Plasmid

#91851

Purpose

Luciferase reporter for IL2RA enhancer (IGI-P0346)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

IL2RA CaRE4 SNP (plus strand) (IL2RA Human)

Use

Luciferase

Expression

Mammalian

Mutation

rs61839660 C>T

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.23-IL2RA CaRE5 (minus strand)

Plasmid

#91836

Purpose

Luciferase reporter for IL2RA enhancer (IGI-P0613)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

IL2RA CaRE5 (minus strand) (IL2RA Human)

Use

Luciferase

Expression

Mammalian

Mutation

Perfect match to reference sequence

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.23-IL2RA CaRE2 (minus strand)

Plasmid

#91847

Purpose

Luciferase reporter for IL2RA enhancer (IGI-P0624)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

IL2RA CaRE2 (minus strand) (IL2RA Human)

Use

Luciferase

Expression

Mammalian

Mutation

rs6602425 A>C, G>A mutation at chr10:611706…

Available Since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.23-IL2RA CaRE1 (minus strand)

Plasmid

#91838

Purpose

Luciferase reporter for IL2RA enhancer (IGI-P0615)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

IL2RA CaRE1 (minus strand) (IL2RA Human)

Use

Luciferase

Expression

Mammalian

Mutation

Perfect match to reference sequence

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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PB-TET-MKOS

Plasmid

#20959

Purpose

piggyBac vector with tet inducible cMyc, KLF4, Oct4, Sox2 for creation of induced pluripotent stem cells

Depositor

Andras Nagy

Article

Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

Insert

cMyc, KLF4, Oct4, Sox2 (Myc, Klf4, Pou5f1, Sox2 Mouse)

Use

piggybac

Tags

IRES-bGeo

Expression

Mammalian

Available Since

April 23, 2009

Availability

Academic Institutions and Nonprofits only

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pMJ1.19 (AAVS1 RNA donor in pCDNA3.1)

Plasmid

#238007

Purpose

300bp donor RNA complementary to Cas9 cut site at AAVS1 (Addgene plasmid #72833) with intronic sequence intervening the 3bp insertion signature (ATC). Can be used to measure RT-DSBR in human cells.

Depositor

Simon Powell, Agnel Sfeir

Article

Jalan et al Nat Commun. 2025 May 10;16(1):4349. doi:

Insert

AAVS1 (AAVS1, PPP1R12C Human)

Use

Rna donor to study rt-dsbr

Expression

Mammalian

Mutation

3bp insertion signature (ATC) at Cas9 cut site in…

Promoter

CMV

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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6-ISG-IFN

Plasmid

#231488

Purpose

Standard for type I interferon signature (6 genes)

Depositor

Stefano Volpi

Article

Tesser et al Clin Exp Immunol. 2025 Mar 24:uxaf018. d

Inserts

TBP (TBP Human)
IFI27 (IFI27 Human)
IFI44L (IFI44L Human)
IFIT1 (IFIT1 Human)
ISG15 (ISG15 Human)
RSAD2 (RSAD2 Human)
SIGLEC1 (SIGLEC1 Human)
HPRT1 (HPRT1 Human)
18s (RPS18 Human)
G6PD (G6PD Human)

Use

Qpcr plasmid standard

Mutation

partial sequence

Available Since

April 17, 2025

Availability

Academic Institutions and Nonprofits only

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