We narrowed to 167,150 results for: addgene

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TET-pLKO.1 PURO shLuc

Plasmid

#83092

Purpose

Lentiviral shRNA vector for inducible knockdown of luciferase (control hairpin)

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

shLuc

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 5, 2016

Availability

Academic Institutions and Nonprofits only
pPTD-DRBD

Plasmid

#35622

Depositor

Steven Dowdy

Article

Eguchi et al Nat Biotechnol. 2009 Jun;27(6):567-71. E

Insert

PKR DRBD-1

Tags

6xHIS, HA, and TAT

Expression

Bacterial

Promoter

T7

Available Since

March 15, 2012

Availability

Academic Institutions and Nonprofits only
pDDGFP-Leu2d-ScVrg4

Plasmid

#102334

Purpose

Expresses WT ScVrg4 in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

Depositor

Simon Newstead

Article

Parker et al Nature. 2017 Nov 23;551(7681):521-524. d

Insert

YGL225W (VRG4 Budding Yeast)

Tags

GFP

Expression

Yeast

Promoter

GAL1

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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9xHRE-MinTK-CRE

Plasmid

#141146

Purpose

lentiviral expression vector to generate a hypoxia fate-mapping system

Depositor

Daniele Gilkes

Article

Godet et al Nat Commun. 2019 Oct 24;10(1):4862. doi:

Insert

9xHRE-MinTK-CRE

Use

Lentiviral

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pX330-GFP-SFPQ

Plasmid

#97084

Purpose

Encodes an sgRNA that creates a DSB at the promoter of human SFPQ gene.

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

sgRNA GFP-SFPQ

Use

CRISPR

Promoter

U6

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-Purg1

Plasmid

#39349

Depositor

Jurg Bahler

Article

Watt et al PLoS One. 2008 Jan 16;3(1):e1428.

Insert

urg1 promoter (urg1, urg1 Fission Yeast)

Use

Yeast genomic targeting

Tags

kanMX6 module - reverse orientation

Available Since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only
CRISPR_TRAC

Plasmid

#164993

Purpose

Expression of gRNA targeting TCRalpha constant locus

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

gRNA against TRAC

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pRRG389-PC2

Plasmid

#99087

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea prohormone convertase 2 (PC2)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

prohormone convertase 2 (PC2)

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO.1 RagB shRNA #2

Plasmid

#26628

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

RagB shRNA (RRAGB Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only
attB53-Pac-attB53

Plasmid

#183610

Purpose

RMCE vector to shuttle puromycin resistance into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).

Depositor

Sally Lowell

Article

Malaguti et al Development. 2022 May 26. pii: 275525. d

Insert

Pac

Use

Rmce

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-ymVenus-URA3

Plasmid

#168058

Purpose

Replaces Addgene plasmid 118459. Yeast tagging vector to create a gene fusion with yeast optimized mVenus with CaUra3 selection; Contains the linker region at the 5’ end of the FP

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymVenus

Tags

ymVenus

Expression

Yeast

Available Since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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pSADdeltaG-GFP-ER(T2)CreER(T2)

Plasmid

#32649

Depositor

Edward Callaway

Article

Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

Inserts

GFP
ER(T2)CreER(T2)

Use

Rabies virus

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2012

Availability

Academic Institutions and Nonprofits only
pRG710_lexO-HO_LexA-TF_HIS3MX

Plasmid

#154819

Purpose

Inducible expression of HO endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Article

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

HO endonuclease (HO Budding Yeast)
LexA-ER-B112

Tags

FLAG

Expression

Yeast

Promoter

ACT1 and lexO_4-CYC1_core

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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TET-pLKO.1 PURO shGFP

Plasmid

#83085

Purpose

Lentiviral shRNA vector for inducible knockdown of GFP (control hairpin)

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

shGFP

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pBabe hygro MTH1

Plasmid

#21296

Depositor

Bob Weinberg

Article

Rai et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

MTH1 (NUDT1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

May 29, 2009

Availability

Academic Institutions and Nonprofits only
TET-pLKO.1 PURO shLacZ

Plasmid

#98632

Purpose

Lentiviral shRNA vector for inducible knockdown of LacZ (control hairpin)

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

shLacZ

Use

Lentiviral

Expression

Mammalian

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pBigTB-FlpERT2

Plasmid

#149435

Purpose

tamoxifen-inducible Flp recombinase

Depositor

Primo Schaer

Article

Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

Insert

Flp-ERT2 fusion protein

Use

Cre/Lox and Mouse Targeting

Tags

ERT2

Expression

Mammalian

Promoter

CAG promoter

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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VV246: CoChR(C108S)-GCaMP6f-ER in fck

Plasmid

#58521

Purpose

Expresses CoChR(C108S) fused to GCaMP6f in mammalian cells

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

CoChR(C108S)-GCaMP6f-ER

Use

Lentiviral

Tags

GCaMP6f

Expression

Mammalian

Mutation

changed Cysteine 108 to Serine

Promoter

a-CamKII

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR1A_no_ccDB_mUty

Plasmid

#158570

Purpose

V5-tagged sequence of mUty cloned into entry vector for recombination

Depositor

Christian Deschepper

Article

Deschepper et al Sci Rep. 2020 Sep 10;10(1):14900. doi: 1

Insert

Mus musculus ubiquitously transcribed tetratricopeptide repeat containing, Y-linked (Uty), transcript variant 2 (Uty Mouse)

Use

Lentiviral

Tags

V5

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGEN-SBP-DICER1

Plasmid

#50558

Purpose

purification of Dicer1 from mammalian cells

Depositor

Yukihide Tomari

Article

Betancur et al RNA. 2012 Jan;18(1):24-30. doi: 10.1261/

Insert

Dicer1 (Dicer1 Mouse)

Tags

SBP

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only