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GST-TACC3-His6

Plasmid

#69106

Purpose

For bacterial expression of recombinant human TACC3 tagged with GST and His.

Depositor

Stephen Royle

Publication

Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

Insert

Transforming Acidic Coiled Coil protein 3 (TACC3 Human)

Tags

GST and His6

Expression

Bacterial

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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mEos2-Integrin-Beta1-N-18

Plasmid

#57390

Purpose

Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Publication

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Integrin-Beta1 (ITGB1 Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
Amber N1

Plasmid

#27798

Depositor

Steven Vogel

Publication

Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

Insert

mAmber N1

Expression

Mammalian

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Human SHIP2

Plasmid

#124645

Purpose

Expresses HIS tagged human SHIP2 in COS-7/HEK cells

Depositor

Christophe Erneux

Publication

Pesesse et al J Biol Chem. 2001 Jul 27;276(30):28348-5

Insert

INPPL1 (INPPL1 Human)

Tags

His Xpress (N-terminal)

Expression

Mammalian

Mutation

coding sequence starts at amino acid 18 as calcul…

Available since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223-AKT1

Plasmid

#23752

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

AKT1 (AKT1 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pNF02-mJuniper

Plasmid

#219398

Purpose

Constitutive expression of mJuniper in E. coli

Depositor

Christian Lesterlin

Publication

Fraikin et al Sci Adv. 2025 Apr 18;11(16):eads6201. do

Insert

mJuniper

Expression

Bacterial

Promoter

proDp

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pCRISPRyl_D17

Plasmid

#84611

Purpose

Introduces DSB at D17 locus in Y. lipolytica

Depositor

Ian Wheeldon

Publication

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Promoter

UAS1B8-TEF(136)

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pH2B-CyPet-FHA2-AuroraB Substrate-YPet-IRES-puro2b

Plasmid

#40571

Depositor

Daniel Gerlich

Publication

Wurzenberger et al J Cell Biol. 2012 Jul 23;198(2):173-83.

Insert

Aurora B FRET-sensor N-terminally fused to histone 2B

Tags

CyPet and YPet

Expression

Bacterial and Mammalian

Promoter

CMV

Available since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only

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pFA6a-GFP11-His3MX

Plasmid

#91951

Purpose

Universal C-terminal GFP11 tagging

Depositor

Rong Li

Publication

Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

Type

Empty backbone

Expression

Yeast

Available since

June 7, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-shRNA-Tet1

Plasmid

#85742

Purpose

shRNA against mouse Tet1

Depositor

Hongjun Song

Publication

Yu et al Nat Neurosci. 2015 Jun;18(6):836-43. doi

Insert

shRNA Tet1

Use

AAV

Tags

EYFP

Available since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.1(+)/AUG-DAP5-3XFLAG

Plasmid

#127331

Purpose

Express 3xFLAG tagged DAP5 protein from AUG start codon

Depositor

Jeremy Wilusz

Publication

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

DAP5 (EIF4G2 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3-Flag-SPRTN-wt

Plasmid

#110214

Purpose

Expression in mammalian cells of full-length wild-type SPRTN protein with a Flag-tag at N-terminus.

Depositor

Ivana Marinovic-Terzic, Kristijan Ramadan

Publication

Lessel et al Nat Genet. 2014 Nov;46(11):1239-44. doi:

Insert

SPRTN (SPRTN Human)

Expression

Mammalian

Mutation

P296L (see depositor comments below)

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223-PIK4CB

Plasmid

#23839

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PIK4CB (PI4KB Human)

Use

Gateway Cloning

Mutation

1MM, G799D

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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FRP2350

Plasmid

#127580

Purpose

Tagging plasmid for WTC 846: Encodes the PtetO7.1 promoter and Hyg resistance gene

Depositor

Joerg Stelling

Publication

Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

Insert

PtetO7.1_Flagtag_8Glycine linker

Use

Synthetic Biology; Pcr based tagging

Tags

Flag tag, 8glycine linker

Available since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pRK5 Flag-RagC

Plasmid

#99723

Purpose

Mammalian expression of Flag-RagC

Depositor

David Sabatini, Kuang Shen

Publication

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Insert

RagC (RRAGC )

Tags

Flag

Promoter

CMV

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pBITE-EGFRvIII-F269-OKT3

Plasmid

#190681

Purpose

Encodes an EGFRvIII-CD3 BITE protein (F269 clone)

Depositor

Scott McComb

Publication

Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

Insert

Anti-EGFRvIII-scFv-F269-modularized-OKT3-HisTag

Use

Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Promoter

EFS

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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scFLARE1

Plasmid

#158738

Purpose

Mammalian expression of scFLARE1 component

Depositor

Alice Ting

Publication

Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

Insert

scFLARE1

Use

AAV

Tags

Flag, V5

Expression

Mammalian

Mutation

Truncated form of TEV protease (220-242 amino aci…

Promoter

CMV

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-Flag YAP127/128/131/381A

Plasmid

#27378

Depositor

Kunliang Guan

Publication

Zhao et al Genes Dev. 2010 Jan 1. 24(1):72-85.

Insert

YAP (YAP1 Human)

Tags

2xFLAG

Expression

Mammalian

Mutation

S127/128/131/381A

Available since

March 4, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pRS1422

Plasmid

#12548

Depositor

Robert Sobol

Publication

Trivedi et al Cancer Res. 2005 Jul 15. 65(14):6394-400

Insert

human Methylpurine DNA glycosylase (MPG Human)

Expression

Mammalian

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Lenti-EFS-PEmax-P2A-Puro

Plasmid

#228890

Purpose

Lentiviral delivery and expression of PEmax protein with puromycin selection cassette.

Depositor

Francisco J Sánchez-Rivera

Publication

Gould et al Nat Biotechnol. 2024 Mar 12. doi: 10.103

Inserts

PEmax
Puromycin resistance

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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