We narrowed to 42,599 results for: cha;
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pCfB3051(gRNA X-3 XI-2 XII-2)
Plasmid#73293PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-3, XI-2, and XII-2DepositorInsertguiding RNA
UseCRISPR; GrnaTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGAN200
Plasmid#129203PurposeyTRAP sensor of [PSI+], detects aggregation of Sup35DepositorInsertSup35NM (SUP35 Budding Yeast)
UseSynthetic BiologyTagsExpressionYeastMutationN terminal and middle domainsPromoterSUP35Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn1 ChR2 ET-TC 2A tDimer
Plasmid#101361PurposeHigh efficiency channelrhodopsin, neuron specific promoter, red fluorescent proteinDepositorInsertsChannelrhodopsin-2
synaptophysin-red fluorescent protein
UseAAVTagsExpressionMammalianMutationE123T , T159CPromoterhuman SynapsinAvailable sinceFeb. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-VCPIP (OTU, aa 25-561)
Plasmid#61583PurposeExpresses human VCPIP (OTU domain) in E. coli.DepositorInsertVCPIP (VCPIP1 Human)
UseTagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-24 and aa 562-1222.PromoterT7Available sinceDec. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAVA-LGF2-Gal11p
Plasmid#127481PurposePositive control for the AVA-seq system. LGF2 (lambda CI associated) and Gal11p (RNAp associated) interactionDepositorInsertLGF2-Gal11p (GAL11 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P41nmt1-GFP
Plasmid#39290DepositorInsertnmt1 promoter (nmt1 Fission Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionMutation-1163 to +6 of nmt1 locus with a 4bp deletion (TA…PromoterAvailable sinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUDE735
Plasmid#103024Purposeexpression of a Cpf1 programming crRNA targetting CAN1, HIS4, PDR12 and ADE2 (crCAN1-4.crHIS4-4.crPDR12-3.crADE2-3.S)DepositorInsertcrCAN1-4.crHIS4-4.crPDR12-3.crADE2-3.S
UseCRISPRTagsExpressionYeastMutationPromoterSNR52Available sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAVA-Gal11p-LGF2
Plasmid#127480PurposePositive control for the AVA-seq system. Gal11p (lambda CI associated) and LGF2 (RNAp associated) interactionDepositorInsertGal11p-LGF2 (GAL11 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYES2-gRNA-hyg-MCS
Plasmid#107734PurposeE. coli-S. cerevisiae shuttle plasmid harbors gRNA expression cassettes targeting S. cerevisiae chromosomal X-3 site (HXK1 gene). Can function as a gRNA expression empty backbone plasmid.DepositorInsertX-3 site gRNA expression cassettes
UseCRISPRTagsExpressionYeastMutationPromoterSNR52pAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
Arch(D95H)-eGFP
Plasmid#51081PurposeExpresses Arch(D95H)-eGFP in mammalian cells as a fluorescent reporter of membrane voltageDepositorInsertArchaerhodopsin-3 w/ D95H point mutation fused to eGFP
UseLentiviralTagseGFPExpressionMammalianMutationChanged Aspartic Acid 95 to HistidinePromoterUbiquitinAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCIG-Flag-Sox9-IRES-nls-GFP
Plasmid#44281DepositorInsertFlag-Sox9 (SOX9 Chicken)
UseTagsFlagExpressionMammalianMutationPromoterCMV-IE enhancer and Chicken beta-actin promoterAvailable sinceAug. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUD1 (OTU, aa 287-437)
Plasmid#61407PurposeExpresses human OTUD1 (OTU domain) in E. coli.DepositorInsertOTUD1 (OTUD1 Human)
UseTagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-286 and aa 438-481.PromoterT7Available sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDblet
Plasmid#8848DepositorInsertARS doublet
UseYeast cloning vectorTagsExpressionYeastMutationThis vector contains, between AatII sites, a dire…PromoterAvailable sinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
SNAP-MIDAS
Plasmid#171604PurposeExpresses the SNAP-tagged MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717) in bacteria.DepositorInsertMIDAS domain of S. pombe Mdn1 (a.a. 4381-4717)
UseTagsHis6 tag and SNAP tagExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSBL_DK202
Plasmid#156426PurposeMammalian expression of 3a delta N(aa 42-275)-EGFP with CMV promoter, can be used for transfection or used to generate bacmids for baculoviral infection of mammalian cellsDepositorInsertSARS-CoV-2 3a protein (aa 42-275)
UseTagsEGFP-HisExpressionMammalianMutationaa 42-275PromoterCMVAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUB2 (full-length, aa 1-234)
Plasmid#61421PurposeExpresses human OTUB2 (full-length) in E. coli.DepositorInsertOTUB2 (Ubiquitin thioesterase OTUB2) (OTUB2 Human)
UseTagsHis6-GST-3CExpressionBacterialMutationPromoterT7Available sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P81nmt1-GFP
Plasmid#39291DepositorInsertnmt1 promoter (nmt1 Fission Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionMutation-1163 to +6 of nmt1 locus with a 7bp deletion (AT…PromoterAvailable sinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCDNA6-LRRC52-V5-Flag-His
Plasmid#127818PurposeExpression of human LRRC52 in mammalian cellsDepositorInsertLRRC52 (LRRC52 Human)
UseTagsV5, FLAG, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGS1022
Plasmid#59576PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Twin-Strep tag and a URA3 selection markerDepositorInsertTwin-Strep tag optimized for PCR amplification followed by URA3 selection marker
UseE. coli cloning vectorTagsTwin-Strep tagExpressionMutationPromoterNoneAvailable sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFA0055
Plasmid#131774PurposeGuide RNA (gCASS5a) and Cas9 expression plasmid for cleaving pFA6 series deletion cassettes, including KanMX, hphMX and natMX. Used to perform CRISPR-Swap of alleles.DepositorInsert20mer CASS5a guide (gCASS5a) and 5' sgRNA
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P-1 human HUWE1 WWE (1611-1703)
Plasmid#132614Purposeexpresses human HUWE1 WWE (1611-1703) in E. coliDepositorInsertHUWE1 WWE (1611-1703)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
NET30 pEGFP-N2 (815)
Plasmid#62011Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertNET30 (MOSPD3 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
DTECT - Plasmid for Standard Curve
Plasmid#139333PurposeThis plasmid is used to generate the unique standard curve required to quantify the capture of genomic signature using DTECTDepositorInsertDTECT ligation product
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
NET55 pEGFP-N2 (610)
Plasmid#61992Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertNET55 (APH1B Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
p3A9cbetaTCR.20beta
Plasmid#69592PurposeTCR beta chain genomic transgenic construct for creating OT-II miceDepositorInsertTCR beta VJ region from clone 1.1 (Tcrb Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUD6A (OTU, aa 128-288)
Plasmid#61417PurposeExpresses human OTUD6A (OTU domain) in E. coli.DepositorInsertOTUD6A (OTUD6A Human)
UseTagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-127.PromoterT7Available sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMXs-HOXA9
Plasmid#131608Purposeexpression of human HOXA9 in mammalian cellsDepositorInsertHOXA9 (HOXA9 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUD6B (OTU, aa 133-293)
Plasmid#61419PurposeExpresses human OTUD6B (OTU domain) in E. coli.DepositorInsertOTUD6B (OTUD6B Human)
UseTagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-132.PromoterT7Available sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMXs_FLAG-FSF1
Plasmid#110641PurposeRetroviral expression of flag-tagged yeast FSF1 in mammalian cellsDepositorInsertFSF1 (FSF1 Budding Yeast)
UseRetroviralTagsFLAGExpressionMammalianMutationcodon-optimized cDNA (no amino acid mutations)PromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P41nmt1-GST
Plasmid#39287DepositorInsertnmt1 promoter (nmt1 Fission Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionMutation-1163 to +6 of nmt1 locus with a 4bp deletion (TA…PromoterAvailable sinceSept. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
UASgal-A9-Pleum
Plasmid#60343PurposeContains a promoter of activating sequence derived from native gal promoter coupled with core promoter derived from native Leucine promoter. Spacer between core and activating sequence was used.DepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promote…TagsExpressionYeastMutationPromoterAvailable sinceNov. 6, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOPINK-OTUD2 (OTU, aa 132-314)
Plasmid#61410PurposeExpresses human OTUD2 (OTU domain) in E. coli.DepositorInsertOTUD2 (YOD1 Human)
UseTagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-131 and aa 315-348.PromoterT7Available sinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCfB3053(gRNA X-2, XI-5, XII-4)
Plasmid#73295PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-2, XI-5, and XII-4DepositorInsertguiding RNA
UseCRISPR; GrnaTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMXs_FLAG-ODC2
Plasmid#133257PurposeExpress FLAG-tagged ODC2 in mammalian cellsDepositorInsertODC2 (ODC2 Budding Yeast)
UseRetroviralTags3xFLAGExpressionMammalianMutationcodon-optimized for expression in human cellsPromoterAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMXs_FLAG-NDT2
Plasmid#133255PurposeExpress FLAG-tagged NDT2 in mammalian cellsDepositorInsertNDT2 (YEA6 Budding Yeast)
UseRetroviralTags3xFLAGExpressionMammalianMutationcodon-optimized for expression in human cellsPromoterAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBS human Frataxin (myc tag)
Plasmid#11443DepositorInsertFrataxin (FXN Human)
UseTagsmycExpressionBacterialMutationPromoterAvailable sinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro human Frataxin
Plasmid#11505DepositorInsertFrataxin (FXN Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pHL 1917
Plasmid#53024PurposeAmpR + PCon:RBS (st7)::mCherry::T1 terminator + PLlacO-1::RBS (st7) fimB::Asp terminator + ColE1DepositorInsertsUseSynthetic BiologyTagsAsp terminator, RBS(st7), and T1 terminatorExpressionBacterialMutationPromoterpCon and pLlacO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
QuasAr-P2A-GCaMP
Plasmid#154082PurposeIndependent expression of QuasAr and GCaMP under human synapsin 1 promoterDepositorInsertQuasAr-P2A-GCaMP6f
UseAAVTagsExpressionMammalianMutationChanged Tyrosine 327 to AlaninePromoterhuman synapsin 1Available sinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only
