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We narrowed to 12,157 results for: pcDNA

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Showing: 6801 - 6850 of 12157 results

  1. pcDNA-dCas9-p300 Core (1396/1397 SY/WW)

    Plasmid
    #61363
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation 1396/1397 SY/WW) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, S. pyogenes, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; 1396/1397 SY/WW m…
    Promoter
    CMV
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61363-plasmid-map-sequence-id-99798

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  17. pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3-L928A-M929A

    Plasmid
    #233065
    Purpose
    Expresses tethered-agonist dead mouse ADGRL3-L928A-M929A (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide.
    Depositor
    Jonathan Javitch
    Article
    Perry-Hauser et al J Biol Chem. 2025 Jan 9:108174. doi: 10.
    Insert
    ADGRL3 (Adgrl3 Mouse)
    Tags
    ALFA and HiBiT
    Expression
    Mammalian
    Mutation
    Leucine 928 to Alanine; Methionine 929 to Alanine
    Available Since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    233065-plasmid-map-sequence-id-464575

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  21. pcDNA3.1-myc:Skp1(dC111):gs6:TEVs:gs6:fLuc:HA

    Plasmid
    #228530
    Purpose
    Firefly luciferase in fusion with a fragment of human Skp1 protein through a flexible linker and a TEV viral protease cleavage site. Expression of fLuc protein is deinhibited by TEV site cleavage.
    Depositor
    Roman Jerala
    Article
    Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80
    Insert
    myc:Skp1(dC111):gs6:TEVs:gs6:fLuc:HA (SKP1 Synthetic, Photinus pyralis, Human)
    Tags
    HA and myc
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    228530-plasmid-map-sequence-id-456690

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  47. SSH-pcDNA3.1-SARS-CoV-2-S-Lys1269Ala-His1271Ala

    Plasmid
    #218463
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 spike (1273 residues; Lys1269Ala and His1271Ala substitutions)
    Depositor
    Syed Saif Hasan
    Article
    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:
    Insert
    SARS-CoV-2 spike protein (1273 residues) carrying Lys1269Ala and His1271Ala substitutions (S )
    Expression
    Mammalian
    Mutation
    Lys1269Ala and His1271Ala substitutions
    Available Since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    218463-plasmid-map-sequence-id-436199

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  48. pcDNA5/FRT/TO-N-FLAG-hBirA*-TLK1-M566T

    Plasmid
    #218832
    Purpose
    Expresses N-terminal BirA*-tagged human TLK1 variant 5 mutant M566T for BioID experiments.
    Depositor
    Travis Stracker
    Insert
    TLK1 M566T (TLK1 Human)
    Tags
    FLAG-BirA*
    Expression
    Mammalian
    Mutation
    M566T
    Promoter
    CMV
    Available Since
    June 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    218832-plasmid-map-sequence-id-434422

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  49. pcDNA5/FRT/TO-N-FLAG-hBirA*-TLK1-Q479E

    Plasmid
    #218833
    Purpose
    Expresses N-terminal BirA*-tagged human TLK1 variant 5 mutant Q479E for BioID experiments.
    Depositor
    Travis Stracker
    Insert
    TLK1 Q479E (TLK1 Human)
    Tags
    FLAG-BirA*
    Expression
    Mammalian
    Mutation
    Q479E
    Promoter
    CMV
    Available Since
    June 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    218833-plasmid-map-sequence-id-434421

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  50. pcDNA5/FRT/TO GABARAP(5X/G116A)-mTagBFP2-GABARAP(5X)

    Plasmid
    #218426
    Purpose
    Expression of GABARAP split tandem protein with mTagBFP2, 5X: K2A, K13A, R15A, K20A, K23A
    Depositor
    Dieter Willbold
    Article
    Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.
    Insert
    GABARAP(G116A/5X)-mTagBFP2-GABARAP(5X) (GABARAP Entacmaea quadricolor, Human)
    Tags
    mTagBFP2
    Expression
    Mammalian
    Mutation
    5X: K2A, K13A, R15A, K20A, K23A mutations of both…
    Promoter
    CMV
    Available Since
    May 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    218426-plasmid-map-sequence-id-434963

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Showing: 6801 - 6850 of 12157 results