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We narrowed to 11,711 results for: pcDNA

Showing: 6801 - 6850 of 11711 results

  4. pCDNA-CRY2-mCh-superPLDx100-P2A-CIBN-CAAX

    Plasmid
    #188994
    Purpose
    A plasmid for dual expression of CRY2-mCh-superPLDx100 (an engineered PLD mutant with 100 times higher activity than the wild-type) and plasma membrane-tagged CIBN
    Depositor
    Jeremy Baskin
    Article
    Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415
    Insert
    PLD
    Use
    Tags
    CAAX, CIBN, CRY2, P2A, and mCherry
    Expression
    Mammalian
    Mutation
    K57R, A59V, K109R, P245A, V264I, G328S, G381V, G4…
    Promoter
    CMV
    Available since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    188994-plasmid-map-sequence-id-371647

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  7. pOTTC1755 - pcDNA3.1 CMV-IE hChR2(H134R)-V5-ER-LBD (ChRERa)

    Plasmid
    #201819
    Purpose
    A plasmid expressing channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a CMV promoter
    Depositor
    Christopher Richie
    Article
    Bonaventura et al Sci Transl Med. 2023 Jul 26;15(706):eadd
    Insert
    hChR2(H134R)-V5-ER-LBD (ChRERa)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pOTTC1751
    Available since
    Oct. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    201819-plasmid-map-sequence-id-399602

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  9. (271) pcDNA3.1-HA-nEPEA-(EAAAK)3-OGA(544-706)

    Plasmid
    #168102
    Purpose
    HA- tagged EPEA nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA
    Depositor
    Christina Woo
    Article
    Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/
    Insert
    HA-nEPEA-(EAAAK)3-OGA(544-706)
    Use
    Tags
    HA-tag
    Expression
    Mammalian
    Mutation
    OGA truncated to contain residues 544-706
    Promoter
    T7/CMV
    Available since
    May 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    168102-plasmid-map-sequence-id-333448

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  18. pCDNA-CRY2-mCh-superPLDx10-P2A-CIBN-CAAX

    Plasmid
    #188991
    Purpose
    A plasmid for dual expression of CRY2-mCh-superPLDx10 (an engineered PLD mutant with 10 times higher activity than the wild-type) and plasma membrane-tagged CIBN
    Depositor
    Jeremy Baskin
    Article
    Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415
    Insert
    PLD
    Use
    Tags
    CAAX, CIBN, CRY2, P2A, and mCherry
    Expression
    Mammalian
    Mutation
    F163L, K249R, G328S, G381V, G429D, D480G
    Promoter
    CMV
    Available since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    188991-plasmid-map-sequence-id-371644

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  23. pcDNA3.1-kappa-myc-dL5-2XG4S-mCer3-KDEL

    Plasmid
    #73209
    Purpose
    Expresses myc-dL5(E52D)-mCer3 fusion protein in endoplasmic reticulum of mammalian cells with an Igk-leader. (MBIC5, dL5**, FAP)
    Depositor
    Marcel Bruchez
    Article
    Telmer et al ACS Chem Biol. 2015 May 15;10(5):1239-46
    Insert
    kappa-myc-dL5-2XG4S-mCer3-KDEL (MYC Synthetic, Human)
    Use
    Tags
    The FAP and mCerulean3 are fused with 2 copies of…
    Expression
    Mammalian
    Mutation
    The dL5** FAP is E50D, L89S, also known as E52D/L…
    Promoter
    CMV
    Available since
    March 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    73209-plasmid-map-sequence-id-139734

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  24. pCDNA-CRY2-mCh-superPLDx30-P2A-CIBN-CAAX

    Plasmid
    #188992
    Purpose
    A plasmid for dual expression of CRY2-mCh-superPLDx30 (an engineered PLD mutant with 30 times higher activity than the wild-type) and plasma membrane-tagged CIBN
    Depositor
    Jeremy Baskin
    Article
    Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415
    Insert
    PLD
    Use
    Tags
    CAAX, CIBN, CRY2, P2A, and mCherry
    Expression
    Mammalian
    Mutation
    K109R, P245A, G328S, G381V, G429D
    Promoter
    CMV
    Available since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    188992-plasmid-map-sequence-id-371645

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  29. pCDNA-CRY2-mCh-superPLDx50-P2A-CIBN-CAAX

    Plasmid
    #188993
    Purpose
    A plasmid for dual expression of CRY2-mCh-superPLDx50 (an engineered PLD mutant with 50 times higher activity than the wild-type) and plasma membrane-tagged CIBN
    Depositor
    Jeremy Baskin
    Article
    Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415
    Insert
    PLD
    Use
    Tags
    CAAX, CIBN, CRY2, P2A, and mCherry
    Expression
    Mammalian
    Mutation
    D201G, A258T, K327R, G328S, G381V, G429D, T450A
    Promoter
    CMV
    Available since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    188993-plasmid-map-sequence-id-371646

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  33. (367) pcDNA3.1-HA-nUbc-(EAAAK)4-OGA(544-706)

    Plasmid
    #168099
    Purpose
    HA- tagged Ubc6E nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA
    Depositor
    Christina Woo
    Article
    Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/
    Insert
    HA-nUbc-OGA(544-706)
    Use
    Tags
    HA-tag
    Expression
    Mammalian
    Mutation
    OGA truncated to contain residues 544-706
    Promoter
    T7/CMV
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    168099-plasmid-map-sequence-id-336059

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  44. pcDNA3.1 Flag YFP hNKCC1 N-terminus T,S mutations (NT51)

    Plasmid
    #49061
    Purpose
    Expresses human NKCC1 mutated Ser and Thr residues in the N-terminus and an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    S150N, S155Q, S170Q, T177A, S183R, S193E, T203A, …
    Promoter
    CMV
    Available since
    Nov. 19, 2013
    Availability
    Academic Institutions and Nonprofits only
    49061-plasmid-map-sequence-id-72183

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  45. pcDNA4/TO-myc NES-mCE(K294A) NLS-Flag

    Plasmid
    #82469
    Purpose
    Expresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal flag tagged mRNA capping enzyme, inactive form
    Depositor
    Daniel Schoenberg
    Article
    Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d
    Insert
    mRNA capping enzyme (Rngtt Mouse)
    Use
    Tags
    Flag and Myc
    Expression
    Mammalian
    Mutation
    changed Lysine 294 to Alanine
    Promoter
    CMV
    Available since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    82469-plasmid-map-sequence-id-163073

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Showing: 6801 - 6850 of 11711 results