We narrowed to 4,326 results for: CIT

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pD2sE_SC10

Plasmid

#219716

Purpose

DV2 stable E dimer with fusion loop mutation

Depositor

Brian Kuhlman

Article

Kudlacek et al Sci Adv. 2021 Oct 15;7(42):eabg4084. doi

Insert

DV2 sE SC10

Tags

8xHis and human serum albumin (HSA)

Expression

Mammalian

Mutation

G106D, A259W, T262R, F279W, T280P

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1 mTagBFP2 rat DJ-1 shRNA

Plasmid

#222870

Purpose

Expresses mTagBFP2 along with an shRNA against rat DJ-1

Depositor

Timothy Ryan

Article

Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

Insert

DJ-1 shRNA (Park7 Rat)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-U6-msCAMK2d-sgRNA-cTNT-SaCas9-HA-OLLAS

Plasmid

#209782

Purpose

Expresses SaCas9 by the specific cTNT promoter and sgRNA targeted the mouse Camk2d gene by U6 promoter

Depositor

Yuxuan Guo

Article

Yang et al Circulation. 2024 May 28;149(22):1778-17

Insert

sgRNA

Use

AAV

Tags

HA and OLLAS

Promoter

cTNT

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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MS2-adRNA (5'UAG)

Plasmid

#170126

Purpose

Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a UAG at the 5' end of the ADAR2 deaminase domain in plasmids #170124 and 170125

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

MS2-ADAR2(5'UAG)-MS2

Use

Lentiviral

Expression

Mammalian

Promoter

Human U6 and mouse U6

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pSPL3-hRPH3A_c.1853Gmut

Plasmid

#190479

Purpose

minigene assay

Depositor

Alfredo Brusco

Article

Pavinato et al Genet Med. 2023 Nov;25(11):100922. doi:

Insert

RPH3A (NM_014954.3) c.1853 [exon 20 and part of surrounding introns only] (RPH3A Human)

Expression

Mammalian

Mutation

c.1853A>G

Promoter

SV40

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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MS2-adRNA (3'UAG)

Plasmid

#170128

Purpose

Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a UAG at the 3' end of the ADAR2 deaminase domain in plasmids #170124 and 170125

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

MS2-ADAR2(3'UAG)-MS2

Use

Lentiviral

Expression

Mammalian

Promoter

Human U6 and mouse U6

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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shNg Lenti FHRC3J1UGW

Plasmid

#92233

Purpose

lentiviral expression of EGFP and Ng shRNA

Depositor

Weifeng Xu

Article

Hwang et al Biol Psychiatry. 2021 Feb 1;89(3):256-26

Insert

shRNA targeting Ng

Use

Lentiviral

Expression

Mammalian

Promoter

H1

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_Caspase8(C360A)-P2A-Hygro_Barcode

Plasmid

#170217

Purpose

Barcoded lentiviral vector to express Caspase8 (C360A) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

CASP8 (CASP8 Human)

Use

Lentiviral

Tags

V5

Mutation

C360A

Promoter

EF1a

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pDestTol2-pax3a-kcnj13-IRES-EGFP

Plasmid

#164950

Purpose

Tol2 construct: pax3a-5.4k promoter driven zebrafish kcnj13 fused with EGFP and EGFP

Depositor

GuangJun Zhang

Article

Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

Insert

kcnj13 (kcnj13 Zebrafish)

Use

Tol2 transposon destination vector

Tags

IRES-EGFP

Promoter

pax3a promoter -5.4k

Available Since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDestTol2-actinb-kcnj13-IRES-EGFP

Plasmid

#164941

Purpose

Tol2 construct: actinb (actb2) promoter driven zebrafish kcnj13 and EGFP

Depositor

GuangJun Zhang

Article

Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

Insert

kcnj13 (kcnj13 Zebrafish)

Use

Tol2 transposon destination vector

Tags

IRES-EGFP

Promoter

actinb (actb2) promoter

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pEF Doc2alpha D291N mutant GFP

Plasmid

#128821

Purpose

To visualize Doc2alpha D291N mutant in PC12 cells

Depositor

Edwin Chapman

Article

Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

Insert

Doc2alpha (Doc2a Rat)

Tags

EGFP

Expression

Mammalian

Mutation

D291N

Promoter

EF-1alpha

Available Since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pEF Doc2beta D303N mutant GFP

Plasmid

#128822

Purpose

To visualize Doc2beta D303N mutant in PC12 cells

Depositor

Edwin Chapman

Article

Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

Insert

Doc2beta (Doc2b Rat)

Tags

EGFP

Expression

Mammalian

Mutation

D303N

Promoter

EF-1alpha

Available Since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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ETV3_pet28a

Plasmid

#131646

Purpose

Bacterial expression of ETS gene ETV3

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ETV3 (ETV3 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pLaNGo11-3

Plasmid

#126054

Purpose

A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Inserts

chloroplastic FNR transit peptide
ccdB cassette (BsaI)
GFP11x3 (5 aa linker)

Use

Binary

Tags

GFP1-10

Expression

Plant

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pMiNGo11-1

Plasmid

#126056

Purpose

A self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Inserts

mitochondria Rieske iron sulphur protein (1-100 aa)
ccdB cassette (BsaI)
GFP11

Use

Binary

Tags

GFP1-10

Expression

Plant

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLJM1-NtermRGS2-GFP-CIBN

Plasmid

#112258

Purpose

Expresses alpha-helix component of opto-RGS2 in mammalian cells

Depositor

Brian Chow

Article

Hannanta-Anan et al ACS Synth Biol. 2018 Jun 15;7(6):1488-14

Insert

NtermRGS2-GFP-CIBN

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR1CB0113

Plasmid

#117552

Purpose

Level 1 Golden Gate Cassette: eSaCas9 expression cassette for dicotyledonous plants

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

2x35S+5'UTR OMEGA (pICH51288) + eSaCas9 (no stop codon) (pEPOR0SP0012) + YFP (pICSL50005) + Nos (pICH41421)

Expression

Plant

Promoter

2x35S+5'UTR OMEGA (pICH51288)

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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CD4-C-mCherry HDRT Source (pTR 175)

Plasmid

#112019

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with mCherry

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

CD4-mCherry HDRT (CD4 Human)

Use

CRISPR and Synthetic Biology

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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mcry1K31R HA tag

Plasmid

#110297

Purpose

mammalian expression of full length mouse CRY1 mutant K31R

Depositor

Joseph Takahashi

Article

Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

Insert

mcry1 K31R (Cry1 Mouse)

Tags

HA tag

Expression

Mammalian

Mutation

31 Lysines are changed to Argenine

Promoter

CMV

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pMTgR2

Plasmid

#107286

Purpose

Plasmid can be used for Drosophila S2 driven secretion of extracellular part of human interferon-_ receptor 2.

Depositor

Bohdan Schneider

Article

Mikulecky et al Acta Crystallogr D Struct Biol. 2016 Sep

Insert

Interferon gamma receptor 2 (IFNGR2 Human)

Tags

CATCATCACCATCACCATGA

Expression

Insect

Promoter

Metallothionein promoter

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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