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pUN1309 - pL2_pNOS-RLUC-tNOS_tOCS-FLUC-pD4H

Plasmid

#203901

Purpose

Reporter plasmid for the D4H promoter from C. roseus with firefly luciferase readout and Renilla luciferase normalization in a plant binary vector for Agrobacterium-mediated transformation.

Depositor

Carolyn Lee-Parsons

Insert

pD4H-FLUC-tOCS; pNOS-RLUC-tNOS

Use

Luciferase

Expression

Plant

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pAN008

Plasmid

#220049

Purpose

Reporter plasmid that encodes for the GFP fused to an N-terminal flag-HA epitope (positive control).

Depositor

Blake Wiedenheft

Article

Nemudraia et al Science. 2024 Apr 25:eadk5518. doi: 10.1

Insert

flag-HA-GFP

Expression

Mammalian

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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MAPOW

Plasmid

#205699

Purpose

shRNAmir backbone for RNA interference, with mOrange reporter

Depositor

Dapeng Wang

Article

Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

Insert

miR-AB

Use

Retroviral

Promoter

MSCV

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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MAPVW

Plasmid

#205698

Purpose

shRNAmir backbone for RNA interference, with Venus reporter

Depositor

Dapeng Wang

Article

Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

Insert

miR-AB

Use

Retroviral

Promoter

MSCV

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBaseline-PRM-wasabi

Plasmid

#195560

Purpose

Always-off, unactivated expression of mWasabi reporter

Depositor

Mikhail Shapiro

Article

Xiong et al ACS Synth Biol. 2022 Jul 15;11(7):2518-2

Insert

mWasabi

Tags

AANDENYADAS ssrA tag

Expression

Bacterial

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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MAPZW

Plasmid

#205694

Purpose

shRNAmir backbone for RNA interference, with Azurite reporter

Depositor

Dapeng Wang

Article

Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

Insert

miR-AB

Use

Retroviral

Promoter

MSCV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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MAPEW

Plasmid

#205701

Purpose

shRNAmir backbone for RNA interference, with E2-Crimson reporter

Depositor

Dapeng Wang

Article

Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

Insert

miR-AB

Use

Retroviral

Promoter

MSCV

Available Since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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MAPCW

Plasmid

#205700

Purpose

shRNAmir backbone for RNA interference, with mCherry reporter

Depositor

Dapeng Wang

Article

Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

Insert

miR-AB

Use

Retroviral

Promoter

MSCV

Available Since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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MAPAW

Plasmid

#205697

Purpose

shRNAmir backbone for RNA interference, with Ametrine reporter

Depositor

Dapeng Wang

Article

Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

Insert

miR-AB

Use

Retroviral

Promoter

MSCV

Available Since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pRegev_D3

Plasmid

#204182

Purpose

Single reporter with long guanine depleted (G-poor) region from splicing library.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

Exon D3

Use

Synthetic Biology

Expression

Mammalian

Promoter

Truncated CAG

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pRegev_S5

Plasmid

#204174

Purpose

Single reporter with secondary structure from splicing library.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

Exon S5 in beta globin splicing minigene

Use

Synthetic Biology

Expression

Mammalian

Promoter

Truncated CAG

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pRegev_S1

Plasmid

#204150

Purpose

Single reporter with secondary structure from splicing library.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

S1 exon in beta globin splicing minigene

Use

Synthetic Biology

Expression

Mammalian

Promoter

Truncated CAG

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pRegev_S4

Plasmid

#204170

Purpose

Single reporter with secondary structure from splicing library.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

Exon S4 in beta globin splicing minigene

Use

Synthetic Biology

Expression

Mammalian

Promoter

Truncated CAG

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pRegev_S2

Plasmid

#204162

Purpose

Single reporter with secondary structure from splicing library.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

S2 exon in beta globin splicing minigene

Use

Synthetic Biology

Expression

Mammalian

Promoter

Truncated CAG

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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sgRNA5_8xCTS-ECFP-pA

Plasmid

#200247

Purpose

Mammalian transfections; 8xCTS-ECFP reporter construct 5

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Insert

sgRNA5_8xCTS

Expression

Mammalian

Available Since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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sgRNA3_8xCTS-ECFP-pA

Plasmid

#200245

Purpose

Mammalian transfections; 8xCTS-ECFP reporter construct 3

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Insert

sgRNA3_8xCTS

Expression

Mammalian

Available Since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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sgRNA2_8xCTS-ECFP-pA

Plasmid

#200244

Purpose

Mammalian transfections; 8xCTS-ECFP reporter construct 2

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Insert

sgRNA2_8xCTS

Expression

Mammalian

Available Since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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sgRNA1 _8xCTS-ECFP-pA

Plasmid

#200243

Purpose

Mammalian transfections; 8xCTS-ECFP reporter construct 1

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Insert

sgRNA1_8xCTS

Expression

Mammalian

Available Since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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sgRNA1 _1xCTS-ECFP-pA

Plasmid

#200237

Purpose

Mammalian transfections; 1xCTS-ECFP reporter construct 1

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Insert

sgRNA1_1xCTS

Expression

Mammalian

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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sgRNA5 _1xCTS-ECFP-pA

Plasmid

#200241

Purpose

Mammalian transfections; 1xCTS-ECFP reporter construct 5

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Insert

sgRNA5_1xCTS

Expression

Mammalian

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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sgRNA3 _1xCTS-ECFP-pA

Plasmid

#200239

Purpose

Mammalian transfections; 1xCTS-ECFP reporter construct 3

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Insert

sgRNA3_1xCTS

Expression

Mammalian

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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gRNA4 _8xCTS-ECFP-pA

Plasmid

#200246

Purpose

Mammalian transfections; 8xCTS-ECFP reporter construct 4

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Insert

sgRNA4_8xCTS

Expression

Mammalian

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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sgRNA4 _1xCTS-ECFP-pA

Plasmid

#200240

Purpose

Mammalian transfections; 1xCTS-ECFP reporter construct 4

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Insert

sgRNA4_1xCTS

Expression

Mammalian

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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sgRNA2 _1xCTS-ECFP-pA

Plasmid

#200238

Purpose

Mammalian transfections; 1xCTS-ECFP reporter construct 2

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Insert

sgRNA2_1xCTS

Expression

Mammalian

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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vYM143_phiC31-nTRE-pEF1s(low)(CG793)-H2b-Citrine

Plasmid

#197686

Purpose

"pEF1s(low)" reporter plasmid, with CpG793 mutated to CG

Depositor

Michael Elowitz

Article

Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

Insert

pEF1s-H2B-Citrine

Use

Synthetic Biology

Expression

Mammalian

Available Since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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vYM010_phiC31-nTRE-pEF1s(high)-H2b-Citrine

Plasmid

#197678

Purpose

"pEF1s(high)" reporter plasmid

Depositor

Michael Elowitz

Article

Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

Insert

pEF1s-H2B-Citrine

Use

Synthetic Biology

Expression

Mammalian

Available Since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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vYM011_phiC31-nTRE-pEF1s(low)-H2b-Citrine

Plasmid

#197679

Purpose

"pEF1s(low)" reporter plasmid

Depositor

Michael Elowitz

Article

Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

Insert

pEF1s-H2B-Citrine

Use

Synthetic Biology

Expression

Mammalian

Available Since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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vYM141_phiC31_nTRE-pEF1s(orig)(CC793)-H2B-citrine

Plasmid

#197684

Purpose

"pEF1s(orig)" reporter plasmid, with CpG793 mutated to CC

Depositor

Michael Elowitz

Article

Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

Insert

pEF1s-H2B-Citrine

Use

Synthetic Biology

Expression

Mammalian

Available Since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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pPRC6

Plasmid

#198429

Purpose

Cloning of promoter of interest in front of mkate2 reporter gene

Depositor

Eveline Peeters

Article

Bernauw et al Microbiologyopen. 2023 Jun;12(3):e1356.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

no promoter

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pEPSW1KN0022

Plasmid

#197538

Purpose

MoClo Level 1 luciferase reporter

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

DREB26::LucN::FLAG::nosT

Use

Synthetic Biology

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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