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Showing: 2741 - 2760 of 7537 results

  3. MSP680

    Plasmid
    #65772
    Purpose
    Human expression vector for SpCas9 EQR variant: CMV-T7-humanSpCas9(D1135E/R1335Q/T1337R)-NLS-3xFLAG (EQR variant)
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E/R1335Q/T1337R)-NLS-3XFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135E, R1335Q, and T1337R mutations in Cas9
    Promoter
    CMV & T7
    Available Since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65772-plasmid-map-sequence-id-112654

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  5. BB44_pmc.DonorR5.TS

    Plasmid
    #199223
    Purpose
    Donor plasmid with CCR5 target sites for ITPN or HMEJ knock-in at the human CCR5 safe harbour locus
    Depositor
    Manuel Goncalves
    Article
    Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.
    Insert
    Expression unit for mCherry - monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)
    Use
    Gene targeting donor plasmid
    Expression
    Mammalian
    Promoter
    Human PGK1 gene promoter
    Available Since
    April 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199223-plasmid-map-sequence-id-391672

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  14. CMV-GEX-GECO1

    Plasmid
    #32443
    Purpose
    Mammalian expression of green excitation ratiometric genetically encoded Ca2+ indicator for optical imaging
    Depositor
    Robert Campbell
    Article
    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.
    Insert
    GEX-GECO1.0
    Expression
    Mammalian
    Mutation
    GCaMP3 K69E/K119I/L173Q/T223S/F315L/F368L/L372Q/K…
    Promoter
    CMV
    Available Since
    Sept. 29, 2011
    Availability
    Academic Institutions and Nonprofits only

  15. pAAV-CaMP0.4-FHS-paCaMKII(SD)-WPRE3

    Plasmid
    #165430
    Purpose
    Expresses light-insensitive photoactivatable CaMKII(SD: Super Dark) in mammalian cells as a negative control.
    Depositor
    Hideji Murakoshi
    Article
    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1
    Insert
    FHS-paCaMKII(SD)
    Use
    AAV
    Tags
    FHS(Flag, HisX6, Strep) tag
    Expression
    Mammalian
    Mutation
    C450A, L514K, G528A, L531E, N538E: Light-insensit…
    Promoter
    CaMKII0.4
    Available Since
    March 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    165430-plasmid-map-sequence-id-325945

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  20. pAAV-TetON-Bmpr1A-ca

    Plasmid
    #217536
    Purpose
    AAV vector to drive the expression of Cre- and doxycycline-dependent expression of HA-epitope tagged constitutively-active BMPR1A receptor
    Depositor
    Peter Scheiffele
    Article
    Okur et al bioRxiv 2023
    Insert
    constitutively active BMP receptor 1a (BMPR1A Human)
    Use
    AAV
    Tags
    HA
    Expression
    Mammalian
    Promoter
    Tet operon (TetO)
    Available Since
    April 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    217536-plasmid-map-sequence-id-429501

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Showing: 2741 - 2760 of 7537 results