161,152 results

1. pHSN6A01

   Plasmid

   #50586

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Inserts

   dCas9-VP64
   gRNA scaffold
   

   Use

   CRISPR; Plant expression

   Tags

   3xFLAG, HA, and NLS

   Expression

   Mutation

   dCas9 = defective in DNA cleavage; 4×minimal VP16…

   Promoter

   2×35Sp and AtU6-26p

   Available since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pXPR_502_sgCD4

   Plasmid

   #158704

   Purpose

   CD4 CRISPRa control

   Depositor

   David Root, John Doench

   Article

   Orchard et al J Virol. 2018 Dec 10;93(1). pii: JVI.013

   Insert

   CD4 (CD4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223-EGFR

   Plasmid

   #23935

   Depositor

   David Root, William Hahn

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   EGFR (EGFR Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti6.3-OGDH_wobble

   Plasmid

   #110938

   Purpose

   OGDH ORF containing silent wobble mutations insensitive to corresponding shRNAs

   Depositor

   William Hahn

   Article

   Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;11

   Insert

   OGDH wobble cDNA (OGDH Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   15 silent wobble mutations corresponding to three…

   Promoter

   SV40

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-Target-ACEmax

   Plasmid

   #139105

   Purpose

   Mammalian Target-ACEmax expression vector (pSI-625)

   Depositor

   Nozomu Yachie

   Article

   Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-anti-TNFalpha(Ozoralizumab) PAGER(FL)

   Plasmid

   #230024

   Purpose

   Expresses anti-TNFalpha PAGER(FL) in mammalian cells

   Depositor

   Alice Ting

   Article

   Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

   Insert

   pAAV-MT3-Ozoralizumab-TEVcs-ALFA-hM4DxcpGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti_P2A_Puro

   Plasmid

   #211364

   Purpose

   Lentiviral expression vector for an insert of interest linked to a P2A-T2A puromycin sequence. Produces virus very efficiently. Can also be used for regular transfections

   Depositor

   Jacques Neefjes

   Article

   Middelburg et al Cell Rep. 2023 Dec 26;42(12):113516. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. iCAP-BI-28-KanR

   Plasmid

   #203532

   Purpose

   RepCap for AAV production

   Depositor

   Ben Deverman

   Article

   Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

   Insert

   AAV-BI28 Cap
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYUB1062-GFP

   Plasmid

   #117695

   Purpose

   Express recombinant proteins with GFP-His fusion in Mycobacterium smegmatis

   Depositor

   Elizabeth Fullam

   Article

   Radhakrishnan et al RSC Adv. 2018 Sep 27;8(58):33087-33095.

   Type

   Empty backbone

   Use

   Tags

   GFP and His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCS2-Cas9

    Plasmid

    #47322

    Purpose

    For in vivo expression of Cas9 or generation of Cas9 mRNA

    Depositor

    Alex Schier

    Article

    Gagnon et al PLoS One. 2014 May 29;9(5):e98186. doi:

    Insert

    Cas9
    

    Use

    CRISPR; Xenopus and zebrafish expression

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6

    Available since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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