We narrowed to 4,867 results for: gne
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Plasmid#167007PurposeMammalian expression of CSL V237ADepositorInsertCSL (RBPJ Human)
UseTagsFLAGExpressionMammalianMutationV237APromoterCMVAvailable sinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3. Flag-CSL F235R
Plasmid#167006PurposeMammalian expression of CSL F235RDepositorInsertCSL (RBPJ Human)
UseTagsFLAGExpressionMammalianMutationF235PromoterCMVAvailable sinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Basic-ZC3H12D_PromoterMUT
Plasmid#153067PurposeZC3H12D promoter region carrying mutations in BHLHE40 binding sites, cloned upstream of luciferase reporterDepositorInsertZC3H12D promoter region mutated in BHLHE40 binding sites (ZC3H12D Human)
UseLuciferaseTagsExpressionMammalianMutationMutagenesis to disrupt 3 BHLHE4040 binding sites.…PromoterNoneAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET21b(+)-Is-PETase-W159H-S238F
Plasmid#112203PurposepET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W159H and S238F mutations, codon optimized for expression in E. coli K12DepositorInsertPETase gene from Ideonella sakaiensis 201-F6 with W159H and S238F mutations, codon optimized for expression in E. coli K12
UseTags6XHISExpressionBacterialMutationW159H, S238FPromoterN/AAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
His-GST-TEV-BRD4(42-459) BD1-BD2
Plasmid#233887PurposepGex for protein overexpression of human BRD4DepositorInsertBRD4 (BRD4 Human)
UseTagsHis-GST-TEVExpressionBacterialMutationPromoterT7Available sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
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pEGFP-N1_c-SRC
Plasmid#171850PurposepEGFP-N1_c-SRCDepositorInsertc-SRC (SRC Human)
UseTagsGFPExpressionMammalianMutationAdded a linker sequence in 3' of c-SRC: GSGSPromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFB-EPCP1
Plasmid#226783PurposeExpression of Z-tagged LRRK2 RCKW using BaculovirusesDepositorInsertLeucine-rich repeat kinase 2 (LRRK2 Human)
UseTagsHis-Z-TEVExpressionInsectMutationAmino acids deleted 1-1326PromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5-LRRK2
Plasmid#229019PurposeExpression of untagged full length human LRRK2 in mammalian cellsDepositorInsertLeucine-rich repeat kinase 2 (LRRK2 Human)
UseTagsno tags (untagged)ExpressionMammalianMutationnonePromoterCMVAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 Sox2 HM a
Plasmid#26353DepositorInsertSox2 shRNA
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 Sox2 3H b
Plasmid#26352DepositorInsertSox2 shRNA
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
GFP-KIF1B beta-600-1400
Plasmid#79109PurposeMammalian expression of GFP-KIF1B beta-600-1400DepositorInsertGFP-KIF1B beta (600-1400 amino acids) (KIF1B Human)
UseTagsGFPExpressionMammalianMutationexon 25 missing (natural splice varient)PromoterCMVAvailable sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS puro HA-hUSP27X
Plasmid#225718PurposeBacterial expression of USP27X with HA tagDepositorInsertUSP27X (USP27X Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPtGE35
Plasmid#107999PurposeExpresses TevCas9 in Phaeodactylum tricornutum / Encodes elements required for conjugationDepositorInsertsOriT
40SRPS8 Promoter
ShBle
40SRPS8 Terminator
Cen6-ArsH4-His3
I-TevI nuclease and partial linker domain
UseCRISPR and Synthetic Biology; Episomal vector for…TagsCas9ExpressionMutationPromoterAvailable sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1α-BHLHE40 aa1-297-IRES-ZsGreen1
Plasmid#153062PurposeLentiviral vector expressing a truncated form of human BHLHE40 (aa 1-297)DepositorInsertTruncated form of human BHLHE40 (aa 1-297) (BHLHE40 Human)
UseLentiviralTagsExpressionMammalianMutationMutagenesis to insert a stop codon at aa 298 in B…PromoterEF1αAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
EFS-SpdCas9-Dnmt3A/3L-catΔ
Plasmid#222501PurposeExpresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant catΔ (C706A and R832E) followed by P2A-mCherryDepositorInsertS. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant catΔ (C706A and R832E) engineered fusion
UseLentiviralTagsFLAG TagExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9; C706A and R832…PromoterEFSAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-Dnmt3a3L-sc27
Plasmid#71827PurposeFor bacterial expression of Dnmt3a-Dnmt3L single-chain fusion protein protein. Contains the catalytic domain of mouse Dnmt3a and the C-terminal part of mouse Dnmt3L connected by 27 aa linker.DepositorInsertDnmt3a catalytic domain (M. musculus) Dnmt3L (M. musculus) C-terminal domain gene fusion (Dnmt3a Mouse)
UseTags6xHis tagExpressionBacterialMutationPromoterAvailable sinceJan. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
CMV-NanoLuc-BRAF-CAT
Plasmid#194772PurposeMammalian expression construct encoding the BRAF catalytic (CAT) domain (AA 435-766) with an N-terminal NanoLuc tag.DepositorInsertBRAF catalytic (CAT) domain (AA 435-766) (BRAF Human)
UseTagsNanoLucExpressionMammalianMutationPromoterCMV51Available sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV-BRAF-REG-Halo
Plasmid#194771PurposeMammalian expression construct encoding the BRAF regulatory (REG) domain (AA 1-435) with a C-terminal HaloTag.DepositorInsertBRAF regulatory (REG) domain (AA 1-435) (BRAF Human)
UseTagsHaloTagExpressionMammalianMutationPromoterCMV51Available sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only