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pGR-ECK125108723

Plasmid

#46008

Depositor

Christopher Voigt

Article

Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

Insert

ECK125108723 Terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

PBAD

Available Since

June 28, 2013

Availability

Academic Institutions and Nonprofits only

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pCS2-SP-FKBP-mCherry-TM-V5

Plasmid

#186226

Purpose

mRNA synthesis of rapalog-induced cell adhesion molecule

Depositor

Yoshihiro Morishita

Article

Usami et al Genes Cells. 2022 Jun;27(6):436-450. doi

Insert

SP-FKBP-mCherry-TM-V5

Tags

V5

Expression

Mammalian

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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p416TEF-SEC16

Plasmid

#102676

Purpose

The gene SEC16 is driven by the strong promoter TEF. Overexpression of SEC16 increases recombinant protein secretion

Depositor

Jens Nielsen

Article

Bao et al Appl Environ Microbiol. 2017 Jun 30;83(1

Insert

SEC16 (SEC16 Budding Yeast)

Expression

Yeast

Promoter

TEF

Available Since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pFA6a-RGS18HIS-hphMX4

Plasmid

#26883

Depositor

Peter Kaiser

Article

Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

Insert

18xHis tag

Use

Pcr tagging yeast

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

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pENTR APLP2 shRNA 1

Plasmid

#30159

Depositor

Dennis Selkoe, Tracy Young-Pearse

Article

Young-Pearse et al Neural Dev. 2008 Jun 23;3:15. doi: 10.11

Insert

APLP2 shRNA 1

Expression

Mammalian

Available Since

July 28, 2011

Availability

Academic Institutions and Nonprofits only
pMT-Mad2-GFP

Plasmid

#24284

Depositor

Ron Vale

Article

Griffis et al J Cell Biol. 2007 Jun 18. 177(6):1005-15

Insert

Mad2 (mad2 Fly)

Tags

GFP

Expression

Insect

Available Since

May 10, 2010

Availability

Academic Institutions and Nonprofits only
pAC1820_pCR8-DR-RG6-SA-DR

Plasmid

#118654

Purpose

Transient transfection; Expressed DR-RG6-SA-DR CasRx gRNA with preprocessed DR 3'; Gateway Donor

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

DR-RG6-SA-DR

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsAGO3_F

Plasmid

#146316

Purpose

Mammalian Expression of HsAGO3

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsAGO3 (AGO3 Human)

Expression

Mammalian

Mutation

T25A

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-zDrosha

Plasmid

#171540

Purpose

Encodes Zebrafish Drosha protein

Depositor

Yan Zeng

Article

Li et al Biochem Biophys Res Commun. 2019 Jun 18;

Insert

drosha (rnasen Zebrafish)

Expression

Mammalian

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pHR Src deltaSH2/3-TagBFP

Plasmid

#171033

Purpose

TagBFP-tagged Src variant lacking SH2 and SH3 domains.

Depositor

Jared Toettcher

Article

Dine et al Cell Rep. 2021 Jun 22;35(12):109280. doi

Insert

Src

Use

Lentiviral

Expression

Mammalian

Available Since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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TRPML2-CFP

Plasmid

#18831

Depositor

Craig Montell

Article

Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

Insert

TRPML2 (Mcoln2 Mouse)

Tags

CFP

Expression

Mammalian

Available Since

July 14, 2008

Availability

Academic Institutions and Nonprofits only
pLNCX Myr His p110

Plasmid

#10836

Depositor

Thomas Roberts

Article

Auger et al Biochem Biophys Res Commun. 2000 Jun 16.

Insert

p110 (PIK3CA Bovine)

Use

Retroviral

Tags

6xHis and Myr

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
mRFP1-36

Plasmid

#63825

Purpose

A member of an RBS library for mRFP1 with different translation rates

Depositor

Howard Salis

Article

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

mRFP1

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

J23100

Available Since

May 15, 2015

Availability

Academic Institutions and Nonprofits only

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EGFP-PML peptide

Plasmid

#175246

Purpose

Bacterial expression and purification, low affinity SUMOylation substrate

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

PML (PML Human)

Expression

Bacterial

Promoter

tac

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pGR-ECK120010800

Plasmid

#46010

Depositor

Christopher Voigt

Article

Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

Insert

ECK120010800 Terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

PBAD

Available Since

June 28, 2013

Availability

Academic Institutions and Nonprofits only

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pCR3.1-OPNc PSM5

Plasmid

#106330

Purpose

Mammalian expression of mutant osteopontin

Depositor

Georg Weber

Article

Shen et al Mol Carcinog. 2014 Jun;53(6):480-7. doi:

Insert

OPNc (SPP1 Human)

Expression

Mammalian

Mutation

OPNc phosphorylation site mutant SGSSEEKQNAVSSEET…

Available Since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pJH577

Plasmid

#172161

Purpose

pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para (pylBCD 3f2); AraC

Expression

Bacterial

Mutation

PylB(N61S, E84K, E122K, E178K, G188R, E251D) PylD…

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pFBXC3H

Plasmid

#49050

Purpose

FX cloning insect cell expression vector

Depositor

Markus Gruetter

Article

Weinert et al Sci Rep. 2015 Jun 4;5:10819. doi: 10.103

Type

Empty backbone

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Insect

Promoter

polH (polyhedrin)

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pFBXNH3

Plasmid

#49049

Purpose

FX cloning insect cell expression vector

Depositor

Markus Gruetter

Article

Weinert et al Sci Rep. 2015 Jun 4;5:10819. doi: 10.103

Type

Empty backbone

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Insect

Promoter

polH (polyhedrin)

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pSL1166

Plasmid

#129520

Purpose

pDEF-SpCas9::GFP, For deletion of pyalba4, PyBiP-full promoter, 2nd Generation

Depositor

Scott Lindner

Article

Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

Insert

SpCas9

Use

Plasmodium, rodent-infectious species

Tags

3XFLAG-NLS

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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