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pCSF107mT-GATEWAY-3'-3HA

Plasmid

#67616

Purpose

A pCS2 based vector to generate a 3'-3HA tag using GATEWAY cloning technology

Depositor

Todd Stukenberg

Publication

Grant et al Dev Biol. 2015 Dec 15;408(2):345-57. doi

Type

Empty backbone

Use

In vitro transcription or translation off of an s…

Tags

three inframe 3' HA tags

Expression

Mammalian

Promoter

CMV and SP6

Available since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pGEX4T-hLIG3

Plasmid

#81055

Purpose

bacterial expression of human LIG3

Depositor

Primo Schaer

Publication

Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

Insert

DNA ligase 3 (LIG3 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMXS-hs-HMGA2

Plasmid

#52727

Purpose

retroviral expression of human HMGA2

Depositor

Shinya Yamanaka

Publication

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

HMGA2 (HMGA2 Human)

Use

Retroviral

Expression

Mammalian

Available since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pJFRC172-10XUAS-loxP>-dSTOP-loxP>-myr::GFP

Plasmid

#32145

Depositor

Gerald Rubin

Publication

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

loxP> STOP STOP loxP>

Expression

Insect

Available since

Sept. 23, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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GFP-Talin-L432G

Plasmid

#26725

Depositor

Anna Huttenlocher

Publication

Franco et al Nat Cell Biol. 2004 Oct . 6(10):977-83.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Talin (Tln1 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Changed Leucine 432 to Glycine to block ability o…

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
GFP-C1-CAMKIIalpha-K42R

Plasmid

#21221

Depositor

Tobias Meyer

Publication

Shen et al Science. 1999 Apr 2. 284(5411):162-6.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CAMKIIalpha-K42R (Camk2a Rat)

Tags

GFP

Expression

Mammalian

Mutation

K42R (Lab plasmid KS 0007).

Available since

July 29, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pSV2neo human IFN-gamma

Plasmid

#10954

Depositor

Howard Young

Publication

Young et al J Immunol. 1986 Jun 15. 136(12):4700-3.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

interferon-gamma (IFNG Human)

Expression

Mammalian

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3-Cyto-GCaMP3

Plasmid

#64853

Purpose

Cytosol-targeted version of the Ca2+ indicator GCaMP3.

Depositor

Jin Zhang

Publication

Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GCaMP3

Tags

Nuclear export signal (NES)

Expression

Mammalian

Promoter

CMV

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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Myc-E-Syt1

Plasmid

#66833

Purpose

Mammalian Expression, Bacterial Expression

Depositor

Pietro De Camilli

Publication

Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

Insert

E-Syt1 (ESYT1 Human)

Tags

Myc

Expression

Mammalian

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pJW1311

Plasmid

#61254

Purpose

Template for cloning sgRNA(F+E) to generate new PU6::sgRNAs by PCR-fusion

Depositor

Jordan Ward

Publication

Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

Insert

sgRNA(F+E) targeting Y61A9L9.1

Use

CRISPR; Cloning template

Available since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBPhsFlp1

Plasmid

#32148

Purpose

Expresses FLP recombinase in Drosophila

Depositor

Gerald Rubin

Publication

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

hsFlp1

Expression

Insect

Mutation

Aspartic Acid at Amino Acid 5

Promoter

Hsp70Bb promoter

Available since

Sept. 23, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLVX_TRE3G_DD::mCherry

Plasmid

#108679

Purpose

Tunable system for controlled gene expression and protein stability in mammalian cells

Depositor

Lucia Marucci

Publication

Pedone et al Nat Commun. 2019 Oct 2;10(1):4481. doi:

Insert

Destabilizing Domain (DD) mCherry fusion protein

Use

Lentiviral

Tags

DD::mCherry fusion protein

Expression

Mammalian

Promoter

Doxycycline inducible

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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FOP-GFP

Plasmid

#35490

Depositor

Ramesh Shivdasani

Publication

Horst et al Cancer Res. 2012 Feb 8.

Insert

7xFOP-GFP

Use

Lentiviral

Expression

Mammalian

Promoter

mutated 7xTCF/LEF

Available since

April 10, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pDEST-HemmarG

Plasmid

#31221

Depositor

Yuh-Nung Jan

Publication

Han et al Proc Natl Acad Sci U S A. 2011 Jun 7;108

Insert

CD4-tdGFP (CD4 Human, Aequorea victoria, Fly)

Tags

CD4 and EGFP/GFP

Expression

Insect

Mutation

Fusion of signal peptide of Drosophila Akh gene, …

Available since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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monomer-NI

Plasmid

#27966

Depositor

Feng Zhang

Publication

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

TAL-monomer-NI

Expression

Bacterial

Available since

Feb. 18, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
monomer-NG

Plasmid

#27967

Depositor

Feng Zhang

Publication

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

TAL-monomer-NG

Expression

Bacterial

Available since

Feb. 18, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pcDNA-1xHA CARM1 WT

Plasmid

#81118

Purpose

mammalian expression of CARM1 WT

Depositor

Sung Hee Baek

Publication

Shin et al Nature. 2016 Jun 15. doi: 10.1038/nature

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CARM1 (Carm1 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

Sept. 28, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
TDP-REGv2(mScarlet-FLAG)#7 in pTwist-CMV backbone

Plasmid

#216152

Purpose

Expresses mScarlet in cells with TDP-43 loss-of-function. This plasmid has one of the best dynamic ranges of those tested (~100-fold) in SK-N-BE2 cells. It uses a cryptic exon. [Code 'A11'] Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

Depositor

Pietro Fratta

Publication

Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mScarlet with cryptic exon and C-terminal FLAG

Expression

Mammalian

Promoter

CMV

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ApoE3

Plasmid

#197147

Purpose

Expresses ApoE3 in Mammalian cell

Depositor

Bradley Hyman

Publication

Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ApoE3 (APOE Human)

Expression

Mammalian

Promoter

CMV

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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Sc++

Plasmid

#155011

Purpose

Expresses the codon-optimized, engineered Sc++ Cas9 nuclease in mammalian cells

Depositor

Joseph Jacobson

Publication

Chatterjee et al Nat Biotechnol. 2020 May 11. pii: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Sc++

Expression

Mammalian

Mutation

T1227K, Loop Substitution from S. anginosus

Promoter

EF1ɑ

Available since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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