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pLVX_TRE3G_DD::mCherry

Plasmid

#108679

Purpose

Tunable system for controlled gene expression and protein stability in mammalian cells

Depositor

Lucia Marucci

Publication

Pedone et al Nat Commun. 2019 Oct 2;10(1):4481. doi:

Insert

Destabilizing Domain (DD) mCherry fusion protein

Use

Lentiviral

Tags

DD::mCherry fusion protein

Expression

Mammalian

Promoter

Doxycycline inducible

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pBABE-HA-GOLPH3

Plasmid

#21687

Depositor

Lynda Chin

Publication

Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

Insert

Golgi Phosphoprotein 3 (GOLPH3 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Strep-KIFC1*-mCh

Plasmid

#120169

Purpose

Expresses a chimera of streptavidin, a KIFC1-based retrograde motor and mCherry (fluorescent tag)

Depositor

Juan Bonifacino

Publication

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member C1 (KIFC1 Human)

Tags

Streptavidin and mCherry

Expression

Mammalian

Mutation

Truncated KIFC1: 125-673 aa

Promoter

CMV

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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KRAB-dCas9

Plasmid

#112195

Purpose

KRAB fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

Depositor

David Segal

Publication

O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

Insert

KRAB (ZNF10 Human)

Use

CRISPR

Tags

3XFLag-NLS-KRAB-dCas9-NLS

Expression

Mammalian

Promoter

CMV

Available since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pcDNA3.1-Flag-PGC-1alpha1

Plasmid

#45501

Depositor

Jorge Ruas, Bruce Spiegelman

Publication

Ruas et al Cell. 2012 Dec 7;151(6):1319-31. doi: 10

Insert

PGC-1alpha Isoform1 (Ppargc1a Mouse)

Tags

6xHis and Flag

Expression

Mammalian

Mutation

Q339K relative to NM_008904

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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NOTCH3-bio-His

Plasmid

#53343

Purpose

Expresses full-length Neurogenic locus notch homolog protein 3 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Publication

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

NOTCH3 (NOTCH3 Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3-ApoE3

Plasmid

#197147

Purpose

Expresses ApoE3 in Mammalian cell

Depositor

Bradley Hyman

Publication

Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

Insert

ApoE3 (APOE Human)

Expression

Mammalian

Promoter

CMV

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pBOB-VE-cadherin-GFP

Plasmid

#118732

Purpose

To express VE-cadherin tagged with GFP on C-terminus

Depositor

John Cooper

Publication

Kim et al Mol Biol Cell. 2018 Jul 15;29(13):1693-1

Insert

Cadherin 5 (CD144) (CDH5 Human)

Use

Lentiviral

Tags

GFP

Mutation

I503T (Common SNP-see comments)

Promoter

CMV

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pCSF107mT-GATEWAY-3'-3HA

Plasmid

#67616

Purpose

A pCS2 based vector to generate a 3'-3HA tag using GATEWAY cloning technology

Depositor

Todd Stukenberg

Publication

Grant et al Dev Biol. 2015 Dec 15;408(2):345-57. doi

Type

Empty backbone

Use

In vitro transcription or translation off of an s…

Tags

three inframe 3' HA tags

Expression

Mammalian

Promoter

CMV and SP6

Available since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
Str-Ii_neomycin

Plasmid

#65312

Purpose

Cytoplasmic ER hook only (RUSH system)

Depositor

Franck Perez

Publication

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Invariant chain (p33)

Expression

Mammalian

Mutation

Streptavidin in its cytoplasmid part

Promoter

CMV

Available since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

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pT2SC

Plasmid

#59382

Purpose

Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a chloramphenicol resistance gene.

Depositor

Shelley Copley

Publication

Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

Insert

TT-ISceI-cat

Use

Template

Available since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRSII425

Plasmid

#35468

Depositor

Steven Haase

Publication

Chee et al G3 (Bethesda). 2012 May;2(5):515-26. Epu

Type

Empty backbone

Use

Yeast 2 micron vector

Promoter

None

Available since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pmRFP-N1 human cofilin S3E

Plasmid

#50858

Purpose

expresses psuedo-phosporylated, non-activatable cofilin fused to mRFP in mammalian cells

Depositor

James Bamburg

Publication

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 S3E (CFL1 Human)

Tags

mRFP

Expression

Mammalian

Mutation

S3E psuedo-phosporylated, non-activatable

Promoter

CMV

Available since

Feb. 20, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
monomer-NI

Plasmid

#27966

Depositor

Feng Zhang

Publication

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

TAL-monomer-NI

Expression

Bacterial

Available since

Feb. 18, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
monomer-NG

Plasmid

#27967

Depositor

Feng Zhang

Publication

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

TAL-monomer-NG

Expression

Bacterial

Available since

Feb. 18, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
CMV-10-3xFLAG-LGP2

Plasmid

#58681

Purpose

Expresses 3x Flag-tagged LGP2 in mammalian cells

Depositor

Curt Horvath

Publication

Bamming et al J Biol Chem. 2009 Apr 10;284(15):9700-12

Insert

laboratory of genetics and physiology 2 (DHX58 Human)

Tags

3x FLAG

Expression

Mammalian

Promoter

CMV

Available since

July 31, 2014

Availability

Academic Institutions and Nonprofits only

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piRFP-N3-Tyrosinase

Plasmid

#80152

Purpose

Melanosome marker

Depositor

Santiago Di Pietro

Publication

Ambrosio et al Proc Natl Acad Sci U S A. 2016 May 17;11

Insert

Tyrosinase (TYR Human)

Tags

iRFP682

Expression

Mammalian

Promoter

CMV

Available since

Aug. 29, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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EGFP-talin1 head

Plasmid

#32856

Depositor

Anna Huttenlocher

Publication

Simonson et al J Immunol. 2006 Dec 1;177(11):7707-14.

Insert

Talin-1 Head aa 1-433 (Tln1 Mouse)

Tags

Enhanced GFP

Expression

Mammalian

Available since

March 6, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pRK5-HA-UFM1

Plasmid

#134639

Purpose

expression of HA-tagged human UFM1 wildtype

Depositor

Yihong Ye

Publication

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

UFM1 (UFM1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLPCX-IRES-GFP

Plasmid

#65436

Purpose

For transfection or retroviral production. Mammalian expression of EGFP driven by the ECMV IRES.

Depositor

Trond Aasen

Publication

Salat-Canela et al Cell Commun Signal. 2014 May 8;12:31. do

Insert

IRES-GFP

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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