We narrowed to 11,682 results for: nts

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DCT.VV.Orf166

Plasmid

#12854

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf166

Expression

Mammalian

Available Since

Dec. 13, 2006

Availability

Academic Institutions and Nonprofits only
pUC19/NLS-mChe

Plasmid

#183162

Purpose

Construct for transient expression of cell nucleus-targeted mCherry in plants.

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Insert

NLS-mCherry

Tags

16 aa nuclear localization signal and 16 aa nucl…

Expression

Plant

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19/ER-mChe

Plasmid

#183163

Purpose

Construct for transient expression of endoplasmic reticulum-targeted mCherry in plants.

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Insert

ER-mCherry

Tags

25 aa ER signal of Glycine soja kunitz-type tryps…

Expression

Plant

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pSLIK 3xFLAG-MAVS hygro

Plasmid

#158634

Purpose

Lentiviral expression vector for an inducible 3xFLAG-tagged MAVS construct

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

MAVS (MAVS Human)

Use

Lentiviral

Tags

3XFLAG

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pET-DUET-1-mRhubarb720-HO

Plasmid

#141201

Purpose

Expresses mRhubarb720 fluorescent protein and heme oxygenase

Depositor

Elad Firnberg

Article

Rogers et al Sci Rep. 2019 Oct 30;9(1):15653. doi: 10

Inserts

mRhubarb720
heme oxygenase

Tags

6x-His

Expression

Bacterial

Mutation

R134H, L196Q, T202D, V203I, W309R, Q310A compared…

Promoter

T7

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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p2T-CAG-REV1-p2A-GFP-PuroR

Plasmid

#153001

Purpose

Overexpression of human REV1

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

REV1 (REV1 Human)

Expression

Mammalian

Promoter

CAG

Available Since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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pBAD/HisD-moxMaple3

Plasmid

#120871

Purpose

Bacterial expression of moxMaple3

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Sci Rep. 2018 Oct 3;8(1):14738. doi: 10.

Insert

moxMaple3

Tags

His

Expression

Bacterial

Mutation

from mMaple3, moxMaple3 was engineered by replaci…

Available Since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-Y-RAN-RFP6

Plasmid

#106424

Purpose

Y-RAN-RFP6 (RANbody RFP6::Chicken IgY)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

Y-RAN-RFP6

Tags

HA and His

Expression

Mammalian

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA_DiCre_59.F2

Plasmid

#60207

Purpose

Expresses a fusion protein of FKBP12-linker peptide 2- Cre(19-59)

Depositor

Jean-Paul Herman

Article

Jullien et al Nucleic Acids Res. 2003 Nov 1;31(21):e13

Insert

Cre recombinase (CD207 Synthetic, Human, Bacteriophage P1)

Tags

FKBP12 and Nuclear localisation signal

Expression

Mammalian

Mutation

amino acids 19-59 of the humanized version of Cre…

Promoter

CMV

Available Since

Jan. 8, 2015

Availability

Academic Institutions and Nonprofits only

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mH2A2-CT-MYC

Plasmid

#45170

Depositor

Brian Chadwick, Hunt Willard

Article

Chadwick et al Hum Mol Genet. 2001 May 1;10(10):1101-13

Insert

H2Afy2 (MACROH2A2, H2AFY2 Human)

Tags

HIS and MYC

Expression

Mammalian

Available Since

July 2, 2013

Availability

Academic Institutions and Nonprofits only
gRNA NIA TLS1

Plasmid

#196980

Purpose

Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Each gRNA is fused to TLS1 mobility signal that confers graft mobility.

Depositor

Friedrich Kragler

Article

Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

AtNIA1 gRNAs fused to TLS1 tags

Expression

Plant

Promoter

pU6-26, pU6-29

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pNH605-HSE-YFP

Plasmid

#172131

Purpose

Fluorescent reporter for Hsf1 activity

Depositor

Johannes Herrmann

Article

Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

Insert

4xHSE-YFP

Use

Vector for genomic integration into the yeast gen…

Expression

Yeast

Promoter

CYC1 core promotor with 4 tandem repeats of the H…

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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loxP-G418-loxP-3xALFA-Halo-Rab11 HR

Plasmid

#229676

Purpose

Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with Halotag and 3x ALFA tag. Contains a G418 resistance cassette for selection of edited cells

Depositor

Francesca Bottanelli

Article

Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

Insert

genomic sequence Rab11 locus (RAB11A Human)

Use

CRISPR

Tags

Halo, 3xALFA

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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2Bc-T MBP_BoMoC(ed)_W403A_F753A_6xH

Plasmid

#185713

Purpose

Recombinant B. mori truncated R2 RT expression

Depositor

Kathleen Collins

Article

Pimentel et al J Biol Chem. 2022 Mar;298(3):101624. doi

Insert

B. mori truncated R2 RT

Tags

6xHis and MBP

Expression

Bacterial

Mutation

Changed W403 and F753 to A

Promoter

T7

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-GAL4-iLight-VP16

Plasmid

#170274

Purpose

NLS-Gal4(1-65)-iLight(human codon optimized)-VP16 construct expression in mammalian cells. pAAV vector.

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

Insert

NLS-Gal4(1-65)-iLight(human codon optimized)-VP16

Use

AAV

Mutation

See Comments

Promoter

CaMKII

Available Since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCC_01 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9-NLS-2A-Puro-WPRE

Plasmid

#139086

Purpose

Expresses human codon-optimized SpCas9 nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

Depositor

Neville Sanjana

Article

Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

Insert

SpCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pMRXIP 3xMyc-STX17ΔNTD

Plasmid

#89940

Purpose

Expresses syntaxin17 lacking N-terminal domain tagged with Myc in mammalian cells

Depositor

Noboru Mizushima

Article

Uematsu et al Autophagy. 2017 Aug 3;13(8):1452-1464. d

Insert

human syntaxin17 lacking N-terminal domain (STX17 Human)

Use

Retroviral

Tags

Myc

Mutation

deleted amino acids 1-161

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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CD 44 pGL4basic

Plasmid

#78135

Purpose

luciferase reporter for miRNA activity

Depositor

Heidi Schwarzenbach

Article

Eichelser et al Oncotarget. 2014 Oct 30;5(20):9650-63.

Insert

CD 44 (CD44 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

CD44

Available Since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

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pTRANS_233

Plasmid

#91122

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: WDV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

35S

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pAID5.3-N

Plasmid

#145812

Purpose

An all-in-one bicistronic plasmid for controlling protein degradation by AID technology

Depositor

Masato Kanemaki

Article

Yesbolatova et al Pharmaceuticals (Basel). 2020 May 23;13(

Type

Empty backbone

Tags

mAID

Expression

Mammalian

Available Since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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