1,878 results

1. pJW1347

   Plasmid

   #154310

   Purpose

   Donor-cassette for SapTrap

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   30aa Linker for CT slot
   

   Use

   CRISPR

   Expression

   Worm

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCC324

   Plasmid

   #110165

   Purpose

   miniMos vector expresses ERKKTR-mClover and mCherry-H2B in C. elegans VPCs; request with plasmids pCC335 and pCC336 for unphosphorylated and phosphomimetic controls (Contains Neomycin resistance)

   Depositor

   Iva Greenwald

   Article

   de la Cova et al Dev Cell. 2017 Sep 11;42(5):542-553.e4.

   Insert

   ERK-KTR-mClover-T2A-mCherry-H2B
   

   Use

   Minimos transposon

   Tags

   mCherry and mClover

   Expression

   Worm

   Mutation

   R61K change made in ERK-KTR CDS

   Promoter

   lin-31 promoter/enhancer

   Available Since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOD2047-bwmControl

   Plasmid

   #100006

   Purpose

   MosSCI targeting vector expressing an ubiquitin ligase adaptor ZIF-1, serving as a control for pOD2046-bwmDEG. Expression is controlled by Pmyo-3 (body wall muscle specific).

   Depositor

   Karen Oegema

   Article

   Wang et al Development. 2017 Jun 15. pii: dev.15009

   Inserts

   zif-1 (zif-1 Nematode)
   unc-119
   

   Use

   Targeting vector for mos1 transposon mediated sin…

   Available Since

   Sept. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCFJ150-GFP(dpiRNA)::CDK-1

   Plasmid

   #107938

   Purpose

   cdk-1p::GFP(dpiRNA)::cdk-1::cdk-1 3'UTR construct used for MosSCI in nematode. GFP is optimized by removing all piRNA targeting sites to allow germline expression.

   Depositor

   Heng-Chi Lee

   Article

   Zhang et al Science. 2018 Feb 2;359(6375):587-592. d

   Insert

   GFP(dpiRNA)::CDK-1 (cdk-1 Nematode)
   

   Expression

   Worm

   Promoter

   cdk-1

   Available Since

   April 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMDJ17 - pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]

   Plasmid

   #159803

   Purpose

   Expression construct with ubiquitous expression to test fluorescence level on microscope

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCFJ178 - cxTi10882_MCS

   Plasmid

   #19331

   Depositor

   Erik Jorgensen

   Article

   Christian et al

   Insert

   cxTi10882 targetting region
   

   Expression

   Worm

   Available Since

   Oct. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pXF276

   Plasmid

   #139048

   Purpose

   MosSCI SapTrap assembly: Cassette 3B for 5-cassette system

   Depositor

   Erik Griffin

   Article

   Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

   Insert

   lov domain (includes STOP codon)
   

   Expression

   Worm

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJF5

   Plasmid

   #139031

   Purpose

   MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

   Depositor

   Erik Griffin

   Article

   Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

   Insert

   gfp (no STOP codon, PATC introns)
   

   Expression

   Worm

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJH4723

   Plasmid

   #178899

   Purpose

   Pges-1 DAF-2 unc-54 3' UTR C.elegans gut expression of of DAF-2

   Depositor

   Mei Zhen

   Article

   Hung et al Development. 2014 Apr;141(8):1767-79. do

   Insert

   DAF-2 (daf-2 Nematode)
   

   Expression

   Worm

   Promoter

   Pges-1

   Available Since

   Jan. 5, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pXF88

    Plasmid

    #139039

    Purpose

    MosSCI SapTrap assembly: Cassette 3 for 4-cassette system

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Insert

    halotag (includes STOP codon, PATC introns)
    

    Expression

    Worm

    Available Since

    March 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJH23

    Plasmid

    #159314

    Purpose

    Punc-25 GFP::SYD-2

    Depositor

    Mei Zhen

    Article

    Yeh et al J Neurosci. 2005 Apr 13;25(15):3833-41.

    Insert

    syd-2 (syd-2 Nematode)
    

    Tags

    GFP

    Expression

    Worm

    Promoter

    Punc-25

    Available Since

    Sept. 22, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pAP973-1

    Plasmid

    #99496

    Purpose

    TEV::linker::meGFP:3XFlag

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Methods. 2017 May 15;121-122:86-93. doi:

    Insert

    TEV::linker::meGFP::3XFlag
    

    Expression

    Bacterial

    Available Since

    Sept. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCG150

    Plasmid

    #17247

    Depositor

    Geraldine Seydoux

    Article

    Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

    Insert

    unc-119 rescue fragment in pDESTR4R3
    

    Use

    Gateway

    Available Since

    March 28, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCFJ1955

    Plasmid

    #84830

    Purpose

    Minimos transposon with Peft-3:tdTomato:tbb-2 3'UTR and cbr-unc-119 selection. tdTomato was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and synthetic introns

    Depositor

    Andrew Fire

    Article

    Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10

    Insert

    C. elegans codon optimized tdTomato
    

    Expression

    Bacterial and Worm

    Promoter

    Peft-3

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJJS011

    Plasmid

    #188334

    Purpose

    PCR template for repair templates to tag a gene with a mTagBFP2 and mIAA7 AID degron using homology-directed repair for C. elegans.

    Depositor

    Mike Boxem

    Article

    Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

    Insert

    mIAA7::mTagBFP2::Linker
    

    Expression

    Bacterial

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDD04neo pmyo2::gfp

    Plasmid

    #26393

    Depositor

    Denis Dupuy

    Article

    Giordano-Santini et al Nat Methods. 2010 Sep;7(9):721-3. doi: 1

    Type

    Empty backbone

    Expression

    Bacterial and Worm

    Available Since

    Oct. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. BSP610

    Plasmid

    #122255

    Purpose

    expression vector for mtagBFP2 under EFT3 promoter

    Depositor

    Alexander Mendenhall

    Article

    Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

    Insert

    mtagBFP2
    

    Expression

    Worm

    Promoter

    EFT3

    Available Since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSDH52

    Plasmid

    #139049

    Purpose

    MosSCI SapTrap assembly: Cassette 3B for 5-cassette system

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Insert

    epdz (includes STOP codon)
    

    Expression

    Worm

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. KG#901

    Plasmid

    #110936

    Purpose

    Expresses Emerald-RAB-3 in the cholinergic motor neuron DA9 for imaging synaptic vesicles in a single neuron in a living animal

    Depositor

    Kenneth Miller

    Article

    Edwards et al Genetics. 2018 Nov;210(3):947-968. doi:

    Inserts

    mig-13 promoter
    Emerald-RAB-3
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    

    Expression

    Bacterial

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJW1583

    Plasmid

    #121054

    Purpose

    GFP^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    GFP^SEC^AID*::3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFLAG, C. elegans codon optimized GFP, and auxin…

    Expression

    Worm

    Available Since

    June 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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