We narrowed to 1,344 results for: SUMO

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pFl Strep-sumo-TEV-hAgo2

Plasmid

#136235

Purpose

Expresses human Argonaute-2 in insect cells

Depositor

Leemor Joshua-Tor

Article

Elkayam et al Cell. 2012 Jul 6;150(1):100-10. doi: 10.

Insert

human Argonaute-2 (AGO2 Human)

Tags

Strep Sumo

Expression

Insect

Promoter

polyhedrin

Available Since

May 14, 2020

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-sumo-NSP12 (SARS-CoV-2)

Plasmid

#159107

Purpose

The SARS-CoV-2 NSP12 coding sequence was cloned into a modified pRSFDuet-1 vector (Novagen) bearing an N-terminal His6-SUMO-tag which is cleavable by the ubiquitin-like protease (ULP1).

Depositor

Thomas Tuschl

Article

Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

Insert

NSP12

Tags

His-SUMO tag

Expression

Bacterial

Mutation

None

Promoter

T7

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pET29-bdSUMO-Anti-ALFA nanobody

Plasmid

#212311

Purpose

Expression of anti-ALFA nanobody

Depositor

Arthur Laganowsky

Article

Stover et al J Struct Biol X. 2024 Feb 2;9:100097. do

Insert

anti-ALFA nanobody

Tags

bdSUMO

Expression

Bacterial

Promoter

T7

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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TRIPZ-CTID195-GSQ-SUMO1nc-PURO

Plasmid

#208035

Purpose

Enables inducible expression of C-terminal Split-TurboID-fused SUMO1nc, to perform SUMO-ID; nc = non-cleavable mutation; selection with puromycin

Depositor

Rosa Barrio, James Sutherland

Article

Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

Insert

SUMO1nc (SUMO1 Human)

Use

Lentiviral

Tags

Myc, C-terminal Split-TurboID

Mutation

Q94P Mutation near C-terminus suppresses cleavage…

Promoter

tetO/UBC

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pAG001 His6-TwinStrep-SUMO-AapCas12b

Plasmid

#153162

Purpose

Bacterial expression plasmid for AapCas12b

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Joung et al medRxiv. 2020 May 8. doi: 10.1101/2020.0

Insert

AapCas12b

Expression

Bacterial

Available Since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

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pET-28a-His6-SUMO-VAMP2

Plasmid

#135551

Purpose

Express mouse VAMP2 in E. coli BL21, purified via HisPur Ni-NTA Resin

Depositor

Jingshi Shen

Article

Yu et al Methods Mol Biol. 2019;1860:237-249. doi

Insert

VAMP2

Tags

6xHis tag, thrombin recognition and cleavage site…

Expression

Bacterial

Mutation

No ATG start codon

Promoter

T7 promoter

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pET30-HPF1-His-Sumo-Flag

Plasmid

#111577

Purpose

Expresses full length HPF1 in E. coli

Depositor

Thomas Muir

Article

Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

Insert

HPF1 (HPF1 Human)

Tags

6xHis-Sumo-FLAG

Expression

Bacterial

Promoter

T7

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pR0306 CcaCas13b His6-TwinStrep-SUMO

Plasmid

#182687

Purpose

Expresses CcaCas13b with N terminal 6xHis-SUMO tag for recombinant bacterial expression and purification

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

CcaCas13b

Tags

SUMO

Expression

Bacterial

Promoter

T7

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pET-30-His6-SUMO-AEY-CfaN

Plasmid

#122949

Purpose

Bacterial expression of N-intein

Depositor

Thomas Muir

Article

Stevens et al J Am Chem Soc. 2016 Feb 24;138(7):2162-5

Insert

CfaN

Expression

Bacterial

Available Since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-sumo-h-cGASCD(K427E/K428E)

Plasmid

#127162

Purpose

expression of truncated human cGAS protein

Depositor

Thomas Tuschl

Article

Lama et al Nat Commun. 2019 May 21;10(1):2261. doi:

Insert

MB21D1 (CGAS Human)

Tags

His-SUMO tag

Expression

Bacterial

Mutation

None

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pAV106-pTDH3-mCherry-10xSumo-6xSIM

Plasmid

#116928

Purpose

Yeast integrative vector for expression of the SUMO-SIM complex

Depositor

Liam Holt

Article

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

10xSUMO-6xSIM

Tags

mCherry

Expression

Yeast

Promoter

TDH3

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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His SUMO TEV WT SPOP 28-359

Plasmid

#194115

Purpose

Wild type His-SUMO SPOP expression plasmid

Depositor

Tanja Mittag

Article

Thomasen et al Elife. 2023 Mar 1;12:e84147. doi: 10.755

Insert

SPOP (SPOP Human)

Tags

6His-SUMO-TEV

Expression

Bacterial

Available Since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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His SUMO TEV SPOP 1-374

Plasmid

#197015

Purpose

E. coli expression vector

Depositor

Tanja Mittag

Article

Cuneo et al Mol Cell. 2023 Mar 2;83(5):731-745.e4. d

Insert

SPOP (Spop Mouse)

Tags

6His-SUMO-TEV

Expression

Bacterial

Available Since

April 5, 2023

Availability

Academic Institutions and Nonprofits only

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pET-His6-Sumo-CfaC-MBP

Plasmid

#126018

Purpose

Bacterial expression of CfaC-MBP fusion protein (with N-terminal His6-Sumo)

Depositor

Thomas Muir

Insert

His6-Sumo-CfaC-MBP

Tags

6xHis-SUMO and HA-MBP

Expression

Bacterial

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pRSF-DUET1-DHDDS/Sumo-NgBR

Plasmid

#203162

Purpose

Expression of human cis-prenyltransferase complex for purification

Depositor

William Sessa

Article

Edani et al Proc Natl Acad Sci U S A. 2020 Aug 25;11

Inserts

DHDDS (DHDDS Human)
NUS1 dehydrodolichyl diphosphate synthase subunit (NUS1 Human)

Tags

His6-SUMO

Expression

Bacterial

Mutation

truncation

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETNKI-his-SUMO-RNF168ring (1-189)

Plasmid

#63570

Purpose

Expression of human RNF168 ring domain (residues 1-189) tagged with His-SUMO

Depositor

Titia Sixma

Article

Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

Insert

RNF168 (RNF168 Human)

Tags

6xHis and SUMO3

Expression

Bacterial

Mutation

Ring domain only (residues 1-189)

Promoter

T7

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
His-SUMO- flag-hDDI2-D252N_pET11a

Plasmid

#169123

Purpose

expression of protein

Depositor

Barbara Dirac-Svejstrup

Article

Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

Insert

hDDI (DDI2 Human)

Expression

Bacterial

Mutation

D252N

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pET-His6-Sumo-MBP-CfaN

Plasmid

#126016

Purpose

Bacterial expression of MBP-CfaN fusion protein (with N-terminal His5-Sumo)

Depositor

Thomas Muir

Insert

His5-Sumo-MBP-CfaN

Tags

5xHis-SUMO

Expression

Bacterial

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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H6SUMO-IntC-P53(304-393)

Plasmid

#200316

Purpose

bacterial expression of P53 TET-CTD for segmental labeling

Depositor

Peter Wright

Article

Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

Insert

p53 (304-393) (TP53 Human)

Tags

His6-SUMO and designed intein C

Expression

Bacterial

Promoter

T7

Available Since

May 31, 2023

Availability

Academic Institutions and Nonprofits only

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pET-28a-His6-SUMO-VAMP3

Plasmid

#135552

Purpose

Express rat VAMP3 in E. coli BL21, purified via HisPur Ni-NTA Resin

Depositor

Jingshi Shen

Article

Yu et al Methods Mol Biol. 2019;1860:237-249. doi

Insert

VAMP3

Tags

6xHis tag, thrombin recognition and cleavage site…

Expression

Bacterial

Mutation

No ATG start codon

Promoter

T7 promoter

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pET-His6-Sumo-IntNtr-eSrt2A

Plasmid

#126015

Purpose

Bacterial expression of IntNtr-eSrt2A fusion protein (with N-terminal His6-Sumo)

Depositor

Thomas Muir

Insert

His6-Sumo-IntNtr-eSrt2A

Tags

6xHis-SUMO

Expression

Bacterial

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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H14-MBP-SUMO-FLAG-LEM

Plasmid

#101778

Purpose

Expresses His-MBP-tagged mutant LEM-domain (FLAG-tagged) in E.coli (SUMO-cleavable)

Depositor

Daniel Gerlich

Article

Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

Insert

MAN1 (LEMD3 Human)

Tags

His14-MBP-SUMO-FLAG

Expression

Bacterial

Mutation

amino acids 6-51 of human MAN1 (LEM domain)

Promoter

T5

Available Since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pET-28a-His6-SUMO-VAMP8

Plasmid

#135553

Purpose

Express mouse VAMP8 in E. coli BL21, purified via HisPur Ni-NTA Resin

Depositor

Jingshi Shen

Article

Yu et al Methods Mol Biol. 2019;1860:237-249. doi

Insert

VAMP8

Tags

6xHis tag, thrombin recognition and cleavage site…

Expression

Bacterial

Mutation

No ATG start codon

Promoter

T7 promoter

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a SUMO Nup53xl 162-267

Plasmid

#86002

Purpose

bacterial expression of his6 and SUMO tagged RRM motif of Xenopus laevis Nup53

Depositor

Wolfram Antonin

Type

Empty backbone

Tags

SUMO

Expression

Bacterial

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pBACgus_Sumostar/LRRK2 1867-2161_C2024S/C2025S

Plasmid

#40394

Depositor

Michael J Fox Foundation MJFF

Inserts

SUMO
LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

6xHis and HisTEV

Mutation

aa1867-2161,C2024S/C2025S

Promoter

polH

Available Since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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p6H-SUMO3-ORF24-NTD-Strep

Plasmid

#138467

Purpose

Expresses SUMO3-tagged ORF24-NTD-Strep in bacteria

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Tags

SUMO3 and Strep-Tag II

Expression

Bacterial

Mutation

Amino acids 2-201

Promoter

T7

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pET-28a-His6-SUMO-RABIF

Plasmid

#135556

Purpose

Express human RABIF in E. coli BL21, purified via HisPur Ni-NTA Resin

Depositor

Jingshi Shen

Article

Gulbranson et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

Insert

RABIF (RABIF Human)

Tags

6xHis tag, thrombin recognition and cleavage site…

Expression

Bacterial

Promoter

T7 promoter

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pAJS1228 His-Sumo-Tau-1-172

Plasmid

#250751

Purpose

Vector for bacterial expression of tau 1-172 with scarless SMNT3/Ulp1 cleavage

Depositor

Martin Kampmann

Article

Samelson et al Cell. 2026 Jan 28:S0092-8674(25)01487-4.

Insert

Tau (MAPT Human)

Tags

6xHis-Sumo

Expression

Bacterial

Mutation

aa 1-172 only

Promoter

T7

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pAJS1229 His-Sumo-Tau-1-176

Plasmid

#250752

Purpose

Vector for bacterial expression of tau 1-176 with scarless SMNT3/Ulp1 cleavage

Depositor

Martin Kampmann

Article

Samelson et al Cell. 2026 Jan 28:S0092-8674(25)01487-4.

Insert

Tau (MAPT Human)

Tags

6xHis-Sumo

Expression

Bacterial

Mutation

aa 1-176 only

Promoter

T7

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pAJS1085 pET His-Sumo-ORF

Plasmid

#250758

Purpose

Vector for bacterial expression of proteins with scarless SMNT3/Ulp1 cleavage

Depositor

Martin Kampmann

Article

Samelson et al Cell. 2026 Jan 28:S0092-8674(25)01487-4.

Type

Empty backbone

Expression

Bacterial

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge