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Plasmid#31748DepositorInsertKhc73 (Khc-73 Fly)
UseTagsGFPExpressionMammalianMutationContains only aa 1-432PromoterMetallothioneinAvailable sinceNov. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pQmod3C-GG
Plasmid#191350PurposeClostridium expression vector (pCB102 origin, cmR) with drop-out Plac-RFP cassette flanked with BsaI sitesDepositorInsertPlac-RFP (IPTG-inducible red fluorescent protein)
UseTagsExpressionBacterialMutationPromoterPlacAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
Flag p21 WT
Plasmid#16240DepositorInsertp21 (CDKN1A Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
pQmod2C-GG
Plasmid#191347PurposeClostridium expression vector (pBP1 origin, cmR) with drop-out Plac-RFP cassette flanked with BsaI sitesDepositorInsertPlac-RFP (IPTG-inducible red fluorescent protein)
UseTagsExpressionBacterialMutationPromoterPlacAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJAK184
Plasmid#167281PurposeMazF-based mutagenesis vector for C. difficileDepositorTypeEmpty backboneUseE. coli - c. difficile shuttle vectorTagsExpressionMutationPromoterPxylAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
PDGFR-TMD (MTK 3B) (pZYW025)
Plasmid#194218PurposeMammalian Toolkit part 3b encoding the transmembrane domain of PDGFRDepositorInsertPDGFR TMD
UseSynthetic BiologyTagsExpressionMutationPromoterNoneAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
T2A-mCherry (MTK 4a) (pAN3844)
Plasmid#194223PurposeMammalian Toolkit part 4a encoding a T2A polycistronic element followed by mCherryDepositorInsertT2A-mCherry
UseSynthetic BiologyTagsExpressionMutationPromoterNoneAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQmod2E-GG
Plasmid#191345PurposeClostridium expression vector (pBP1 origin, ermR) with drop-out Plac-RFP cassette flanked with BsaI sitesDepositorInsertPlac-RFP (IPTG-inducible red fluorescent protein)
UseTagsExpressionBacterialMutationPromoterPlacAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQmod3E-GG
Plasmid#191348PurposeClostridium expression vector (pCB102 origin, ermR) with drop-out Plac-RFP cassette flanked with BsaI sitesDepositorInsertPlac-RFP (IPTG-inducible red fluorescent protein)
UseTagsExpressionBacterialMutationPromoterPlacAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQmod4E-GG
Plasmid#191351PurposeClostridium expression vector (pCD6 origin, ermR) with drop-out Plac-RFP cassette flanked with BsaI sitesDepositorInsertPlac-RFP (IPTG-inducible red fluorescent protein)
UseTagsExpressionBacterialMutationPromoterPlacAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCB-CBEv2
Plasmid#221139PurposeCoxiella burnetii CRISPR-Cas9 cytosine base editing plasmid version 2 without sgRNA construct. Expresses 3xF-BE4-PpAPOBEC1(H122A).DepositorInsert3xF-PpAPOBEC1(H122A)-Cas9n-UGI-UGI (BE4-PpAPOBEC1(H122A))
UseCRISPRTags3xFLAGExpressionBacterialMutationPromoterlacIq-PtacAvailable sinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJAK080
Plasmid#167279PurposeCodA-based vector for insertion of DNA by recombination downstream of the pyrE gene in C. difficileDepositorTypeEmpty backboneUseE. coli - c. difficile shuttle vectorTagsExpressionMutationPromoterAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQmod2S-GG
Plasmid#191346PurposeClostridium expression vector (pBP1 origin, specR) with drop-out Plac-RFP cassette flanked with BsaI sitesDepositorInsertPlac-RFP (IPTG-inducible red fluorescent protein)
UseTagsExpressionBacterialMutationPromoterPlacAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQmod3S-GG
Plasmid#191349PurposeClostridium expression vector (pCB102 origin, specR) with drop-out Plac-RFP cassette flanked with BsaI sitesDepositorInsertPlac-RFP (IPTG-inducible red fluorescent protein)
UseTagsExpressionBacterialMutationPromoterPlacAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
Flag p21 T145A
Plasmid#16241DepositorInsertp21 (CDKN1A Human)
UseTagsFlagExpressionMammalianMutationT145APromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
Flag p21 T145D
Plasmid#16242DepositorInsertp21 (CDKN1A Human)
UseTagsFlagExpressionMammalianMutationT145DPromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
SynNotch TMD/Gal4-VP64 ICD (MTK 3b) (pAN1031)
Plasmid#194222PurposeMammalian Toolkit part 3b encoding SynNotch and GAL4-VP64 to be used in designing modular receptorsDepositorInsertSynNotch-GAL4 DBD-VP64 AD
UseSynthetic BiologyTagsExpressionMutationPromoterNoneAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF702
Plasmid#209650PurposeSuicide plasmid carrying a neomycin resistance marker flanked by homologous regions of the Mth60-fimbria encoding operon of Methanothermobacter thermautotrophicusDepositorInsertsDownstream homologous region
thermostable neomycin phosphotransferase gene
Upstream homologous region
UseTagsExpressionMutationPromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
mito-PDCD10-mScarlet-I
Plasmid#208887PurposeExpress mScarlet-I-PDCD10 targeted to the mitochondria in mammalian cells.DepositorInsertCCM3 (PDCD10 Human)
UseTagsmScarlet-IExpressionMammalianMutationPDCD10 targeted to the mitonchondriaPromoterAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMM7
Plasmid#201213PurposeIntegrative GFP-based shuttle vector for genetic engineering of Parageobacillus thermoglucosidasius. Carries homologous flanks for deletion of key sporulation regulator spo0A.DepositorInsertssfGFP
Spo0A right flank
Spo0A left flank
UseSynthetic BiologyTagsExpressionBacterialMutationF11V, T13P, N39D, A179APromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
LifeAct-mGreenLantern
Plasmid#164459PurposeLocalization of mGreenLantern fluorescent protein to actinDepositorInsertmGreenLantern
UseTagsLifeActExpressionMammalianMutationClover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…PromoterCMVAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPalmitoyl-mTurquoise2
Plasmid#36209PurposeIn vivo visualization of the membrane (can be used for colocalization studies)DepositorInsertp63(1-29) (ARHGEF25 Human)
UseTagsmTurquoise2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
SS-Myc-HexaPro-Foldon (MTK 3a) (pZYW077)
Plasmid#194233PurposeMammalian Toolkit part 3a encoding Jason McClellan lab's 6-proline Spike extracellular domainDepositorInsertCD8a Signal Sequence-Myc Tag-SARS-CoV-2 Spike1-1258 (S Synthetic)
UseSynthetic BiologyTagsExpressionMutationPromoterNoneAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
Hy_U5:34A eGFP SV40
Plasmid#195064PurposeExpresses eGFP when there is readthrough transcription from the Drosophila U5:34A geneDepositorInsertsnRNA:U5:34A (snRNA:U5:34A Fly)
UseTagsExpressionInsectMutationPromoterU5:34AAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
mito-CCM2-mScarlet-I
Plasmid#208886PurposeExpress mScarlet-I-CCM2 targeted to the mitochondria in mammalian cells.DepositorInsertCCM2 (CCM2 Human)
UseTagsmScarlet-IExpressionMammalianMutationCCM2 targeted to the mitonchondriaPromoterAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
mTagBFP2-FKBP-CYB5Atail
Plasmid#108141PurposeFor recruitment of ERDepositorUseTagsExpressionMammalianMutationCYB5A(100-134)PromoterCMVAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEMS1593
Plasmid#29270PurposePleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).DepositorInsertPle122 (ICMT Human)
UsePleiades promoter project [sic, pleaides plieades]TagsIntron-LacZ-NLSExpressionMutationPromoterAvailable sinceOct. 28, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEMS1595
Plasmid#29272PurposeExpression of the reporter gene was not detected in the brain and eye using LacZ.DepositorInsertPle124 (ICMT Human)
UsePleiades promoter project [sic, pleaides plieades]TagsIntron-LacZ-NLSExpressionMutationPromoterAvailable sinceNov. 16, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEMS1594
Plasmid#29271PurposePleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748) and de Leeuw et al., MTM, 2014 (PMID: 24761428).DepositorInsertPle123 (ICMT Human)
UsePleiades promoter project [sic, pleaides plieades]TagsIntron-LacZ-NLSExpressionMutationPromoterAvailable sinceOct. 28, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits