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Plasmid#186460PurposeExpresses human alpha-catenin (residues 22-906) with an N-terminal His tag and a PRescission Protease cleavage siteDepositorInsertCTNNA1 (CTNNA1 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-CsHPAD_beta-gamma
Plasmid#226981PurposeExpressing Clostridium scatologenes hydroxyphenylacetate decarboxylase (HPAD) beta/gamma subunits in E. coliDepositorInsertCsHPAD_beta-gamma
UseTagsHis6 (on backbone)ExpressionBacterialMutationPromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-OuIAD(W392A)
Plasmid#226980PurposeExpressing Olsenella uli indoleacetate decarboxylase (IAD) W392A variant in E. coliDepositorInsertOuIAD(W392A)
UseTagsHis6 (on backbone)ExpressionBacterialMutationW392APromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-OuIAD(W392F)
Plasmid#226979PurposeExpressing Olsenella uli indoleacetate decarboxylase (IAD) W392F variant in E. coliDepositorInsertOuIAD(W392F)
UseTagsHis6 (on backbone)ExpressionBacterialMutationW392FPromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-OuIAD(F401A)
Plasmid#226978PurposeExpressing Olsenella uli indoleacetate decarboxylase (IAD) F401A variant in E. coliDepositorInsertOuIAD(F401A)
UseTagsHis6 (on backbone)ExpressionBacterialMutationF401APromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-OuIAD(L616E)
Plasmid#226977PurposeExpressing Olsenella uli indoleacetate decarboxylase (IAD) L616E variant in E. coliDepositorInsertOuIAD(L616E)
UseTagsHis6 (on backbone)ExpressionBacterialMutationL616EPromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-OuIAD(R226K)
Plasmid#226976PurposeExpressing Olsenella uli indoleacetate decarboxylase (IAD) R226K variant in E. coliDepositorInsertOuIAD(R226K)
UseTagsHis6 (on backbone)ExpressionBacterialMutationR226KPromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-OuIAD(R226M)
Plasmid#226975PurposeExpressing Olsenella uli indoleacetate decarboxylase (IAD) R226M variant in E. coliDepositorInsertOuIAD(R226M)
UseTagsHis6 (on backbone)ExpressionBacterialMutationR226MPromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-OuIAD(R226E)
Plasmid#226974PurposeExpressing Olsenella uli indoleacetate decarboxylase (IAD) R226E variant in E. coliDepositorInsertOuIAD(R226E)
UseTagsHis6 (on backbone)ExpressionBacterialMutationR226EPromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-OuIAD(H514E)
Plasmid#226972PurposeExpressing Olsenella uli indoleacetate decarboxylase (IAD) H514E variant in E. coliDepositorInsertOuIAD(H514E)
UseTagsHis6 (on backbone)ExpressionBacterialMutationH514EPromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-OuIAD(H514A)
Plasmid#226971PurposeExpressing Olsenella uli indoleacetate decarboxylase (IAD) H514A variant in E. coliDepositorInsertOuIAD(H514A)
UseTagsHis6 (on backbone)ExpressionBacterialMutationH514APromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-OuIAD(C500S)
Plasmid#226970PurposeExpressing Olsenella uli indoleacetate decarboxylase (IAD) C500S variant in E. coliDepositorInsertOuIAD(C500S)
UseTagsHis6 (on backbone)ExpressionBacterialMutationC500SPromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-OuIAD(G853A)
Plasmid#226969PurposeExpressing Olsenella uli indoleacetate decarboxylase (IAD) G853A variant in E. coliDepositorInsertOuIAD(G853A)
UseTagsHis6 (on backbone)ExpressionBacterialMutationG853APromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-NEDD4L_W4-HECT
Plasmid#187710PurposeExpress his-tagged NEDD4L W4-HECT in bacterial cellsDepositorInsertNEDD4L residues 543-975 (NEDD4L Human)
UseTagsHis-HRV 3C siteExpressionBacterialMutationdeletion of residues 1-542PromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-NEDD4L_W3-W4-HECT_C874only
Plasmid#187711PurposeExpress his-tagged NEDD4L W3-W4-HECT_C874only in bacterial cellsDepositorInsertNEDD4L residues 492-975 with mutations (NEDD4L Human)
UseTagsHis-HRV 3C siteExpressionBacterialMutationdeletion of residues 1-491; mutations C702A, C776…PromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanIV-Streptomyces
Plasmid#226250PurposeExpresses precursor peptide and lanthipeptide synthetase from Streptomyces LanIV (FAST-RiPPs) in E. coliDepositorInsertLanA (LanIV-Streptomyces)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-a(+)-6xHis-mScarlet-I(+5)
Plasmid#219418PurposeFluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysateDepositorInsertmScarlet-I(+5)
UseTagsMGHHHHHGGExpressionBacterialMutationD125K, D186K, E216K, E238KPromoterT7Available sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanIV-Chondromyces
Plasmid#225078PurposeExpresses precursor peptide and lanthipeptide synthetase from Chondromyces LanIV (FAST-RiPPs) in E. coliDepositorInsertLanA (LanIV-Chondromyces)
UseTagsHis6ExpressionBacterialMutationWTPromoterT7Available sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanI-101
Plasmid#225065PurposeExpresses precursor peptide, lanthipeptide cyclase, and lanthipeptide dehydratase from LanI-101 (FAST-RiPPs) in E. coliDepositorInsertLanA (LanI-101)
UseTagsHis6ExpressionBacterialMutationWTPromoterT7Available sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-a(+)-6xHis-mBlueberry(-9)
Plasmid#219416PurposeFluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysateDepositorInsertmBlueberry2(-9)
UseTagsMGHHHHHGGExpressionBacterialMutationK137E, K199E, R234EPromoterT7Available sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SpyCatcher -AVI
Plasmid#223635PurposeProtein purification of SpyCatcher003 (S49C) with AVI and 6xHis tags from E. coliDepositorInsertSpyCatcher003 S49C
UseTagsAVI tag and His6ExpressionBacterialMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-FKBP-empty
Plasmid#221332PurposeProtein overexpression in bacteriaDepositorInsert6xHis-3xFKBP empty scaffold
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ChmN-C47A
Plasmid#221367PurposeExpress C47A ChmN in E. coliDepositorInsertchmN-C47A
UseTagsHis6ExpressionBacterialMutationchanged Cys 47 to AlaPromoterAvailable sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3L-TwStrep
Plasmid#208649Purposefor E. coli expression of C-ter Twin Strep tagged human RPL3L (ENST00000268661), contain SNP rs753338989, rs746044959, rs752134949DepositorInserthuman RPL3L (ENST00000268661) (RPL3L Human)
UseTags6xHis and Twin StrepExpressionBacterialMutationcontain rs753338989, rs746044959, rs752134949, pr…PromoterT7Available sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-Flag-RBM24
Plasmid#208621Purposefor E. coli expression of Flag tagged human RBM24 (ENST00000379052)DepositorInserthuman RBM24 (ENST00000379052) coding region (RBM24 Human)
UseTags6xHis and FlagExpressionBacterialMutationPromoterT7Available sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_human-pro-IL-18_E192K_D193K
Plasmid#214322PurposeProtein overexpression in bacteriaDepositorInsertIL18 (IL18 Human)
UseTagsHis-TEVExpressionBacterialMutationE192K_D193KPromoterT7Available sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_human-pro-IL-18_I48N
Plasmid#214323PurposeProtein overexpression in bacteriaDepositorInsertIL18 (IL18 Human)
UseTagsHis-TEVExpressionBacterialMutationI48NPromoterT7Available sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_Caspase-4_sheep_catalytic domain
Plasmid#214331PurposeProtein overexpression in bacteriaDepositorInsertCASP4
UseTagsHis-TEVExpressionBacterialMutationPromoterT7Available sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_Caspase-11_humanized-mutant
Plasmid#214315PurposeProtein overexpression in bacteriaDepositorInsertCASP4 (Casp4 Mouse)
UseTagsHis-TEVExpressionBacterialMutationN152K_KLS288YKT_KASIHS348TPRAKAPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_Caspase-11_rat_catalytic domain
Plasmid#214329PurposeProtein overexpression in bacteriaDepositorInsertCASP4 (Casp4 Rat)
UseTagsHis-TEVExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_human-pro-IL-18_LES33AAA
Plasmid#214326PurposeProtein overexpression in bacteriaDepositorInsertIL18 (IL18 Human)
UseTagsHis-TEVExpressionBacterialMutationLES33AAAPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_human-pro-IL-18_DDEN29ENGD
Plasmid#214325PurposeProtein overexpression in bacteriaDepositorInsertIL18 (IL18 Human)
UseTagsHis-TEVExpressionBacterialMutationDDEN29ENGDPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_human-pro-IL-18_V47N_I48N
Plasmid#214324PurposeProtein overexpression in bacteriaDepositorInsertIL18 (IL18 Human)
UseTagsHis-TEVExpressionBacterialMutationV47N_I48NPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_Caspase-4_lemur_catalytic domain
Plasmid#214328PurposeProtein overexpression in bacteriaDepositorInsertCASP4
UseTagsHis-TEVExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_Caspase-4_rabbit_catalytic domain
Plasmid#214330PurposeProtein overexpression in bacteriaDepositorInsertCASP4
UseTagsHis-TEVExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28b-TEV-AcrIC9
Plasmid#207409PurposeProtein purification vector to produce AcrIC9 in E. coliDepositorInsertT7-TEV-AcrIC9
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DONSON-DEWS
Plasmid#208799PurposeXenopus laevis His6-Myc-DONSON (D374A, E377A, W381A, S437A)DepositorInsertDONSON (donson.L Frog)
UseTagsHis and MycExpressionBacterialMutationD374A; E377A; W381A; S437APromoterT7Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DONSON-Δ24
Plasmid#208801PurposeXenopus laevis His6-Myc-DONSON (1-24aa removed)DepositorInsertDONSON (donson.L Frog)
UseTagsHis and MycExpressionBacterialMutationΔ24, deletion of 1-24aaPromoterT7Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DONSON-DEW
Plasmid#208802PurposeXenopus laevis His6-Myc-DONSON (D374A, E377A, W381A)DepositorInsertDONSON (donson.L Frog)
UseTagsHis and MycExpressionBacterialMutationD374A; E377A; W381APromoterT7Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DONSON-S437A
Plasmid#208803PurposeXenopus laevis His6-Myc-DONSON (S437A)DepositorInsertDONSON (donson.L Frog)
UseTagsHis and MycExpressionBacterialMutationS437APromoterT7Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DONSON-ΔD1
Plasmid#208804PurposeXenopus laevis His6-DONSON (1-155aa removed)DepositorInsertDONSON (donson.L Frog)
UseTagsHisExpressionBacterialMutationΔD1, deletion of 1-155aaPromoterT7Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-6xHis-TrxF
Plasmid#207454PurposeE. coli expression of Mesembryanthemum crystallinum Thioredoxin F with thrombin-cleavable N-terminal 6xHis tagDepositorInsertThioredoxin F
UseTags6xHisExpressionBacterialMutationCodon optimized for E coli expressionPromoterT7Available sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-Flag-RBM24-1~93
Plasmid#208633Purposefor E. coli expression of truncated Flag tagged human RBM24 (ENST00000379052), aa1~93DepositorInserthuman RBM24 (ENST00000216146) aa 1~93 (RBM24 Human)
UseTags6xHis and FlagExpressionBacterialMutationonly aa 1~93, other residues not includedPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-eIF4E-TwStrep
Plasmid#208638Purposefor E. coli expression of C-ter Twin Strep tagged human eIF4E (ENST00000450253)DepositorInserthuman eIF4E (ENST00000450253) (EIF4E Human)
UseTags6xHis and Twin StrepExpressionBacterialMutationPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-Twstrep
Plasmid#208623Purposefor E. coli expression of C-ter Twin Strep tagged human RPL3 (ENST00000216146)DepositorInserthuman RPL3 (ENST00000216146) (RPL3 Human)
UseTags6xHis and Twin StrepExpressionBacterialMutationPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-hXRCC1pd-HIS
Plasmid#206036PurposeBacterial expression of PAR-deficient hXRCC1pd with 6xHis tag, under the control of T7 promoterDepositorInserthXRCC1
UseTagsHISExpressionBacterialMutationS103A,R186A,S184A,S193A,S219A,S220A,S236A,S268APromoterT7Available sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pet28b-His_RAD23A-UBA2
Plasmid#201550PurposeExpresses UBA2 of human RAD23A in E. coliDepositorInsertUV excision repair protein RAD23 homolog A (RAD23A Human)
UseTags6x HisExpressionBacterialMutationExpresses amino acids 311-363 of DNA polymerase i…PromoterT7Available sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28 mePOU-POU
Plasmid#206392PurposeExpresses the DNA binding domain of the ePOU with a 6xHis tag for protein purificationDepositorInsertePOU
UseTags6xHisExpressionBacterialMutationPOU DNA binding domain onlyPromoterAvailable sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28 mOct4-POU
Plasmid#206393PurposeExpresses the DNA binding domain of the Oct4 with a 6xHis tag for protein purificationDepositorInsertOct4 (Pou5f1 Mouse)
UseTags6xHisExpressionBacterialMutationPOU DNA binding domain onlyPromoterAvailable sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a LZ control-FLAG
Plasmid#199678PurposeBacterial expression of Leucine Zipper (LZ) control-FlagDepositorInsertLeucine zipper control
UseTags6xHis and FlagExpressionBacterialMutationPromoterT7 promoterAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only