Skip to main content
Addgene
Showing: 261 - 280 of 8294 results

  2. FGFR2 K659M 3xFlag

    Plasmid
    #110112
    Purpose
    expresses FGFR2 with a single amino acid change in mammalian cells
    Depositor
    Martin Fernandez-Zapico
    Insert
    fibroblast growth factor receptor 2 (FGFR2 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    mutated Lysine 659 to Methiionine (K659M)
    Promoter
    CMV
    Available since
    May 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    110112-plasmid-map-sequence-id-209788

    Enlarge

  3. FGFR2 E565A 3xFlag

    Plasmid
    #110109
    Purpose
    expresses FGFR2 with a single amino acid change in mammalian cells
    Depositor
    Martin Fernandez-Zapico
    Insert
    fibroblast growth factor receptor 2 (FGFR2 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    mutated Glutamic acid 565 to Alanine (E565A)
    Promoter
    CMV
    Available since
    May 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    110109-plasmid-map-sequence-id-209786

    Enlarge

  4. FGFR2 K641R 3xFlag

    Plasmid
    #110111
    Purpose
    expresses FGFR2 with a single amino acid change in mammalian cells
    Depositor
    Martin Fernandez-Zapico
    Insert
    fibroblast growth factor receptor 2 (FGFR2 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    mutated Lysine 641 to Arginine (K641R)
    Promoter
    CMV
    Available since
    May 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    110111-plasmid-map-sequence-id-209784

    Enlarge

  5. FGFR2 N549H 3xFlag

    Plasmid
    #110106
    Purpose
    expresses FGFR2 with a single amino acid change in mammalian cells
    Depositor
    Martin Fernandez-Zapico
    Insert
    fibroblast growth factor receptor 2 (FGFR2 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    mutated Asparagine 549 to Histidine (N549H)
    Promoter
    CMV
    Available since
    May 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    110106-plasmid-map-sequence-id-209779

    Enlarge

  6. FGFR2 N549K 3xFlag

    Plasmid
    #110107
    Purpose
    expresses FGFR2 with a single amino acid change in mammalian cells
    Depositor
    Martin Fernandez-Zapico
    Insert
    fibroblast growth factor receptor 2 (FGFR2 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    mutated Asparagine 549 to Lysine (N549K)
    Promoter
    CMV
    Available since
    May 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    110107-plasmid-map-sequence-id-209777

    Enlarge

  7. FGFR2 L617V 3xFlag

    Plasmid
    #110110
    Purpose
    expresses FGFR2 with a single amino acid change in mammalian cells
    Depositor
    Martin Fernandez-Zapico
    Insert
    fibroblast growth factor receptor 2 (FGFR2 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    mutated Leucine 617 to Valine (L617V)
    Promoter
    CMV
    Available since
    May 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    110110-plasmid-map-sequence-id-209775

    Enlarge

  8. CMYA5tnT/pDEST26-N-FLAG

    Plasmid
    #98131
    Purpose
    Express CMYA5 (aa 3859 – 4069) Leu allele (4063) with a N-terminal FLAG tag in pDEST26 mammalian expression vector
    Depositor
    Rita Shiang
    Insert
    CMYA5 (CMYA5 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    rs10043986 (NM_153610.4:c.12188C>T)
    Promoter
    CMV
    Available since
    Aug. 3, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    98131-plasmid-map-sequence-id-190024

    Enlarge

  9. pDownstream1Lox

    Plasmid
    #164574
    Purpose
    Integration of 1 LoxN site in the genome of P. berghei
    Depositor
    Olivier Silvie
    Type
    Empty backbone
    Use
    Cre/Lox
    Tags
    Expression
    Mutation
    Promoter
    Bidirectional PbEF1alpha promoter (PBANKA_1133300…
    Available since
    March 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    164574-plasmid-map-sequence-id-325425

    Enlarge

  10. pUpstream2Lox

    Plasmid
    #164573
    Purpose
    Integration of 2 LoxN sites in the genome of P. berghei
    Depositor
    Olivier Silvie
    Inserts
    Green fluorescent protein - human dihydrofolate reductase (GFP-2A-hDHFR) (DHFR Synthetic, Human)
    bifunctional yeast enzyme cytosine deaminase and uridyl phosphoribosyl transferase (yFCU) (FCY1, FUR1 Budding Yeast)
    Use
    Cre/Lox
    Tags
    Expression
    Mutation
    Promoter
    Bidirectional PbEF1alpha promoter (PBANKA_1133300…
    Available since
    March 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    164573-plasmid-map-sequence-id-325424

    Enlarge

  11. pLenti-PGK-c-myc-2A-CDK4(R24C)-GFP-Puro

    Plasmid
    #83107
    Purpose
    3rd generation lentiviral expression of C-myc, CDK4(R24C), and GFP separated by 2A self-cleaving peptide
    Depositor
    Andrew Brooks
    Inserts
    c-Myc (MYC Human)
    CDK4 (CDK4 Human)
    eGFP
    Use
    Lentiviral
    Tags
    2A self cleaving peptide
    Expression
    Mammalian
    Mutation
    R24C and p64 isoform
    Promoter
    hPGK and hPGK (single transcript)
    Available since
    July 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    83107-plasmid-map-sequence-id-330062

    Enlarge

  12. pSPIKE-F

    Plasmid
    #101174
    Purpose
    chimeric spike resembling fungal ITS region to be used in metagenomics
    Depositor
    Philip Poole
    Insert
    chimeric fungal ITS
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    101174-plasmid-map-sequence-id-194321

    Enlarge

  13. pSPIKE-P

    Plasmid
    #101172
    Purpose
    chimeric spike resembling V4 region of prokaryotic 16S rRNA to be used in metagenomics
    Depositor
    Philip Poole
    Insert
    V4 16S rRNA chimeric spike
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    101172-plasmid-map-sequence-id-194323

    Enlarge

  14. pSPIKE-E

    Plasmid
    #101173
    Purpose
    chimeric spike resembling a fragment of eukaryotic 18S rRNA to be used in metagenomics
    Depositor
    Philip Poole
    Insert
    chimeric 18S rRNA
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    101173-plasmid-map-sequence-id-194324

    Enlarge

  15. pCS2+mScarlet3-C Cloning Vector

    Plasmid
    #220616
    Purpose
    To express a protein of interest fused to the C-terminus of mScarlet3 (unpublished)
    Depositor
    Ken-Ichi Takemaru
    Type
    Empty backbone
    Use
    Tags
    mScarlet3 fusion
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    220616-plasmid-map-sequence-id-435943

    Enlarge

  16. pCMV-AAVS1-TALEN-L

    Plasmid
    #159299
    Purpose
    TALEN (left) targeting the AAVS1 locus with obligate heterodimer FokI
    Depositor
    Kosuke Yusa
    Insert
    TALEN targeting the AAVS1
    Use
    TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    Sept. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    159299-plasmid-map-sequence-id-311595

    Enlarge

  17. pCMV-AAVS1-TALEN-R

    Plasmid
    #159300
    Purpose
    TALEN (right) targeting the AAVS1 locus with obligate heterodimer FokI
    Depositor
    Kosuke Yusa
    Insert
    TALEN targeting the AAVS1
    Use
    TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    Sept. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    159300-plasmid-map-sequence-id-311596

    Enlarge

  18. pZFN-AAVS1_ELD

    Plasmid
    #159297
    Purpose
    ZFN (left) targeting the AAVS1 locus with obligate heterodimer FokI
    Depositor
    Kosuke Yusa
    Insert
    ZFN targeting the AAVS1 locus
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    Sept. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    159297-plasmid-map-sequence-id-311561

    Enlarge

  19. LentiGuide-Hygro

    Plasmid
    #139462
    Purpose
    Lentiviral vector with gRNA scaffold and hygromycin selectable marker
    Depositor
    Caroline Goujon
    Insert
    no sgRNA inserted; resistance gene: hygroR
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a / U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    139462-plasmid-map-sequence-id-308474

    Enlarge

  20. Flag-APEX3-C Cloning Vector

    Plasmid
    #220615
    Purpose
    To express a protein of interest fused to the C-terminus of Flag-APEX3 for proximity labeling or TEM (unpublished)
    Depositor
    Ken-Ichi Takemaru
    Type
    Empty backbone
    Use
    Tags
    Flag-APEX3 fusion
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    220615-plasmid-map-sequence-id-435941

    Enlarge

Showing: 261 - 280 of 8294 results