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Plasmid#161540PurposeInducible expression of 3xFLAG-NLS-DsDed-ZF1-PEST under the ZF10x6-C promoterDepositorInsert3xFLAG-NLS-DsRed-ZF1-PEST
UseSynthetic BiologyTags3x-FLAGExpressionMammalianMutationPromoterZF10x6-CAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM529 EF1α 3xFLAG-NLS-DsDed-intC-ZF10-NLS-HA in TUPV5
Plasmid#161555PurposeConstitutive expression of 3xFLAG-NLS-DsDed-intC-ZF10-NLS-HA under the EF1α promoterDepositorInsert3xFLAG-NLS-DsDed-intC-ZF10-NLS-HA
UseSynthetic BiologyTags3x-FLAG and HAExpressionMammalianMutationPromoterEF1αAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM592 EF1α 3xFLAG-NLS-DsDed-ZF10-intN-VP64 in TUPV2
Plasmid#161568PurposeConstitutive expression of 3xFLAG-NLS-DsDed-ZF10-intN-VP64 under the EF1α promoterDepositorInsert3xFLAG-NLS-DsDed-ZF10-intN-VP64
UseSynthetic BiologyTags3x-FLAGExpressionMammalianMutationPromoterEF1αAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAR012 Lenti pEF-dEnAsCas12a-NLS-ZNF10 KRAB-3XHA-T2A-BSD
Plasmid#195550PurposedCas12a effector fusion for manipulating transcriptionDepositorInsertLenti pEF-dEnAsCas12a-NLS-ZNF10 KRAB-3XHA-T2A-BSD
UseCRISPR and LentiviralTags3XHAExpressionMammalianMutationPromoterpEF1aAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJM590 EF1α 3xFLAG-NLS-VP64-ZF1-intC-ZF10-NLS-HA in TUPV2
Plasmid#161566PurposeConstitutive expression of 3xFLAG-NLS-VP64-ZF1-intC-ZF10-NLS-HA under the EF1α promoterDepositorInsert3xFLAG-NLS-VP64-ZF1-intC-ZF10-NLS-HA
UseSynthetic BiologyTags3x-FLAG and HAExpressionMammalianMutationPromoterEF1αAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJT241 Lenti pEF-dCas9-3XFLAG-WT ZNF10 KRAB-T2A-tagBFP-P2A-Blast
Plasmid#187326PurposedCas9 effector fusion for manipulating transcriptionDepositorInsertdCas9-3XFLAG-WT ZNF10 KRAB-T2A-tagBFP-P2A-Blast
UseCRISPR and LentiviralTags3XFLAGExpressionMammalianMutationPromoterpEF1aAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJT151 lenti pEF-rTetR(SE-G72P)-3XFLAG-ZNF10 KRAB-T2A-mCherry-BSD-WPRE
Plasmid#188757PurposeLentiviral backbone containing ZNF10 KRAB as a fusion with rTetR(SE-G72P)-3XFLAGDepositorInsertZNF10 KRAB (ZNF10 Human)
UseLentiviralTagsrTetR(SE-G72P)-3xFLAGExpressionMammalianMutationPromoterpEF1aAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJT242 Lenti pEF-dCas9-3XFLAG-ZNF10 KRAB[WSR7EEE] HighExpr-T2A-tagBFP-P2A-Blast
Plasmid#187327PurposedCas9 effector fusion for manipulating transcriptionDepositorInsertdCas9-3XFLAG-ZNF10 KRAB[WSR7EEE] HighExpr-T2A-tagBFP-P2A-Blast
UseCRISPR and LentiviralTags3XFLAGExpressionMammalianMutationKRAB WSR7EEEPromoterpEF1aAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
3CEG
Plasmid#60107PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertBIRC6:SDC136-F10:C41228 (BIRC6 Human)
UseTags6xHisExpressionBacterialMutationSee commentsPromoterT7Available sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
4YZ8
Plasmid#69955PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertWHSC1L1:JMC055-F10:C223308 (NSD3 Human)
UseTagsHis and TEV cleavage siteExpressionBacterialMutationPromoterT7-lacOAvailable sinceOct. 7, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
Opto-D2R
Plasmid#106068PurposeExpression of a chimeric putative G-protein coupled receptor (Rhodopsin-D2R; Opto-D2R; F10; 721)DepositorInsertOpto-D2R
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_CUS1C02
Plasmid#71069PurposeGateway entry cloneDepositorInsertTaf10 (Taf10 Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceNov. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAM5505
Plasmid#132664PurposepRL623-based helper plasmid for conjugations, with RSF1010 mobA geneDepositorInsertMobA
UseTagsExpressionBacterialMutationplease see depositor comments belowPromoterp1,p3Available sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAM5404
Plasmid#132660PurposeRSF1010-based broad host-range plasmid with additional RK2 bom siteDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAM5406
Plasmid#132661PurposeRSF1010-based broad host-range plasmid with additional RK2 bom site and pUC originDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
DOT1L-CC3
Plasmid#111754PurposeBacterial expression for structure determinationDepositorInsertDOT1L (DOT1L Human)
UseTags8xHis-Thrombin-SUMO-TEVExpressionBacterialMutationContains coiled coil #3 (residues 613-661)PromoterT7Available sinceJune 14, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
DOT1L-CC1
Plasmid#111756PurposeBacterial expression for structure determinationDepositorInsertDOT1L (DOT1L Human)
UseTags8xHis-Thrombin-SUMO-TEVExpressionBacterialMutationcontains coiled coil #1 (residues 506-555)PromoterT7Available sinceJune 14, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
DOT1L-CC2
Plasmid#111755PurposeBacterial expression for structure determinationDepositorInsertDOT1L (DOT1L Human)
UseTags8xHis-Thrombin-SUMO-TEVExpressionBacterialMutationContains coiled coil #2 (residues 560-608)PromoterT7Available sinceJune 14, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCfb13200
Plasmid#219875PurposeThe base plasmid of TUNEYALI forTF10DepositorInsertContains gRNA targeting TF10 ( YALI1_C16204g) and homologous arm matching TF10
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
Pf-GMPS
Plasmid#36899PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPf-GMPS (PF10_0123 Plasmodium falciparum)
UseTagsHis tag with TEV cleavageExpressionBacterialMutationPromoterAvailable sinceJune 19, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits