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Plasmid#148805PurposeMammalian Expression of HsHelzDepositorInsertHsHelz (HELZ Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
MS2392.EF1a.SLC6A1.WT.EGFP.P2A.BLAST.JL.p164
Plasmid#170169PurposeLentiviral Expression of EF1a.SLC6A1.WT.EGFPDepositorInsertSLC6A1 (SLC6A1 Human)
UseLentiviralTagsExpressionMutationn/aPromoterAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAct:MS2-p65-HSF
Plasmid#78902PurposeExpresses MS2-p65-HSF under actin promoter for SAM CRISPRa in Drosophila cellsDepositorInsertMS2-p65-HSF
UseCRISPRTagsExpressionInsectMutationPromoterpActin (Drosophila)Available sinceSept. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMS25: pTns(PmcCAST)
Plasmid#168158PurposeInducible expression of PmcCAST TnsAB, TnsC, TniQ and TnsD proteins.DepositorInsertsPmcCAST Tns proteins (TnsAB, TnsC and TnsD)
PmcTniQ
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCFD3-MS2tail-SAM
Plasmid#78905PurposeVector for expressing a single sgRNA with SAM MS2 tail, under U6:3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceAug. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
MS2-adRNA (3'UAG)
Plasmid#170128PurposeLentiviral vector carrying 2 copies of the MS2 adRNA targeting a UAG at the 3' end of the ADAR2 deaminase domain in plasmids #170124 and 170125DepositorInsertMS2-ADAR2(3'UAG)-MS2
UseLentiviralTagsExpressionMammalianMutationPromoterHuman U6 and mouse U6Available sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMEH60 Lamp2b MS2 HA
Plasmid#168209PurposeMammalian expression of lysosome-associated membrane protein 2b fused to the MS2 dimer and C-terminal HA tagDepositorInsertLamp2b (LAMP2 Human)
UseTagsHA and MS2 dimerExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
MS2_adRNA-MCP_ADAR1 DD_NLS
Plasmid#124625PurposeExpresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR1 DD_NLSDepositorInsertMCP_ADAR1 DD_NLS
UseAAVTagsNLSExpressionMammalianMutationPromoterCMVAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBCMV-MS2CP-eDHFR
Plasmid#167309PurposeTemplate pDNA to prepare a split CaVT component mRNADepositorInsertMS2CP-eDHFR
UseSynthetic BiologyTagsDYK-tagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 MS2 (GB2665)
Plasmid#160597PurposeCDS of MS2 protein with B3 nomenclatureDepositorInsertMS2
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCFD3-MS2tail-Scaffold
Plasmid#78906PurposeVector for expressing a single sgRNA with Scaffold MS2 tail, under U6:3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceSept. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_3_TOPO_TA-ms2-VP64
Plasmid#79371PurposeRecruits VP64 to a compatible gRNA for transcriptional activationDepositorInsertMS2-VP64
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNX24-RFP-MS2SL
Plasmid#227819PurposeExpresses RFP via CMV promoter. The mRNA is tagged with 24x MS2 stem-loops (version 3) at the 3'UTR. Also encodes Neo resistance geneDepositorInserts3xHA-RFP-24xMS2SL
Neo resistance
UseTagsExpressionMammalianMutationPromoterCMV and SV40Available sinceApril 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pU6-crRNA-MS2
Plasmid#207624PurposeExpresses crRNA-MS2 in mammalian cells (with a human-U6 promoter)DepositorInsertcrRNA-MS2
UseCRISPRTagsExpressionMutationPromoterhU6Available sinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-Puro-2xMS2
Plasmid#212627PurposeMS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particlesDepositorInsertEF1a-PuroR-2xMS2-WPRE-hU6-sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-Puro-12xMS2
Plasmid#212628PurposeMS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particlesDepositorInsertEF1a-PuroR-12xMS2-WPRE-hU6-sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-Puro-4xMS2
Plasmid#212626PurposeMS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particlesDepositorInsertEF1a-PuroR-4xMS2-WPRE-hU6-sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUB_SM-RLuc0---MS2
Plasmid#210572PurposeExpresses SM(FLAG)-nonoptimal Renilla with 3'UTR MS2 hairpinsDepositorInsertSpaghetti monster (FLAG)-nonoptimal Renilla luciferase
UseLentiviral and LuciferaseTagsSpaghetti monster (FLAG)ExpressionMammalianMutationPromoterUBC humanAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUB_SM-RLuc100---MS2
Plasmid#210573PurposeExpresses SM(FLAG)-optimal Renilla with 3'UTR MS2 hairpinsDepositorInsertSpaghetti monster (FLAG)-optimal Renilla luciferase
UseLentiviral and LuciferaseTagsSpaghetti monster (FLAG)ExpressionMammalianMutationPromoterUBC humanAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
3xMS2 TR knockin sgRNA 1
Plasmid#207607PurposesgRNA for homology directed repair insertion of 3xMS2-TR-100-PURO into the endogenous TR locusDepositorInsertcgactcgcccggcagcgcac
UseTagsNoneExpressionMammalianMutationPromoterCMV and U6Available sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only