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myc-mTOR (1-1482 aa)

Plasmid

#21745

Depositor

David Sabatini

Article

Kim et al Mol Cell 2003 Apr;11(4):895-904.

Insert

mTOR (1-1482) (Mtor Rat)

Tags

myc

Expression

Mammalian

Mutation

amino acids 1-1482

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only
pTA-Luc SIRT1 promoter (-202)

Plasmid

#10971

Depositor

Toren Finkel

Article

Nemoto et al Science. 2004 Dec 17. 306(5704):2105-8.

Insert

SIRT1 promoter (Sirt1 Mouse)

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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PU.1-IRES-mCherry

Plasmid

#80140

Purpose

retroviral expression of PU.1 and mCherry

Depositor

Ellen Rothenberg

Article

Kueh et al Science. 2013 Aug 9;341(6146):670-3. doi

Insert

PU.1 (Spi1 Mouse)

Use

Retroviral

Tags

IRES-mCherry

Expression

Mammalian

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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enz.083-NMT1

Plasmid

#154047

Purpose

Expresses NMT1 in E. coli

Depositor

Christopher Voigt

Article

Wallace et al Advanced Functional Materials 2020

Insert

NMT1 (N-myristoyltransferase 1) (NMT1 Human)

Tags

His, MBP

Expression

Bacterial

Promoter

T7

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pL2-1 ConjEpi TpEF2nat TpEF2NoSQEVenusThrFLAG

Plasmid

#139961

Purpose

Over-expression of heterologus squalene epoxidase from Nannochloropsis oceanica

Depositor

Raffaela Abbriano

Article

Jaramillo-Madrid et al Pharmaceuticals (Basel). 2020 Dec 21;13(

Insert

ConjEpi TpEF2_nat_tTpEF2 TpEF2_NoSQEVenusThrFLAG_tTpEF2

Use

Synthetic Biology

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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HBT-GCaMP6-HA

Plasmid

#107433

Purpose

For Arabidopsis mesophyll protoplast transient expression assay

Depositor

Jen Sheen

Article

Liu et al Nature. 2017 May 18;545(7654):311-316. d

Insert

GCaMP6S

Tags

HA

Expression

Bacterial

Available Since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only

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plc242-GFP-Nprl2

Plasmid

#87051

Purpose

mammalian expression of Nprl2

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

Nprl2 (NPRL2 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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pAd-Track SIRT1

Plasmid

#8440

Depositor

Pere Puigserver

Article

Rodgers et al Nature 2005 Mar 3;434(7029):113-8.

Insert

SIRT1 (Sirt1 Mouse)

Use

Adenoviral

Expression

Mammalian

Available Since

Dec. 4, 2007

Availability

Academic Institutions and Nonprofits only

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pAWH

Plasmid

#133894

Purpose

Expression of Gal4AD-Prey hybrid protein. Homology regions for recombination with pBKWH, pBSWHhc or pMS22H

Depositor

Sebastian Maurer

Article

Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

Type

Empty backbone

Expression

Yeast

Available Since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

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pBKWH

Plasmid

#133898

Purpose

Expression of Gal4BD-Bait hybrid protein. Homology regions for recombination with pAWH

Depositor

Sebastian Maurer

Article

Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

Type

Empty backbone

Expression

Yeast

Available Since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

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pKH159_FLAG-APEX2-YIPF4 WT

Plasmid

#214978

Purpose

Flag-APEX2 tagged YIPF4

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

FLAG-APEX2-YIPF4 (YIPF4 Human)

Use

Lentiviral

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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FLAG TBC1D13

Plasmid

#87953

Purpose

Expresses flag tagged mouse TBC1D13

Depositor

David James

Article

Davey et al Traffic. 2012 Oct;13(10):1429-41. doi: 1

Insert

TBC1D13 (Tbc1d13 Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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myc GbetaL mutant 5

Plasmid

#8479

Depositor

David Sabatini

Article

Kim et al Mol Cell 2003 Apr;11(4):895-904.

Insert

GbetaL (MLST8 Human)

Tags

myc

Expression

Mammalian

Mutation

G192D

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
pBSWHhc

Plasmid

#133901

Purpose

Expression of Gal4BD-Bait hybrid protein. Homology regions for recombination with pAWH

Depositor

Sebastian Maurer

Article

Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

Type

Empty backbone

Expression

Yeast

Available Since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

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myc GbetaL mutant 7

Plasmid

#8481

Depositor

David Sabatini

Article

Kim et al Mol Cell 2003 Apr;11(4):895-904.

Insert

GbetaL (MLST8 Human)

Tags

myc

Expression

Mammalian

Mutation

F320S

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
myc GbetaL mutant 2

Plasmid

#8478

Depositor

David Sabatini

Article

Kim et al Mol Cell 2003 Apr;11(4):895-904.

Insert

GbetaL (MLST8 Human)

Tags

myc

Expression

Mammalian

Mutation

S72D

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
pRK5-HA-SZT2 1-1021

Plasmid

#87037

Purpose

mammalian expression of SZT2 fragment

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

SZT2 (SZT2 Human)

Tags

HA

Expression

Mammalian

Mutation

AA 1-1021

Promoter

CMV

Available Since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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myc GbetaL mutant 1

Plasmid

#8477

Depositor

David Sabatini

Article

Kim et al Mol Cell 2003 Apr;11(4):895-904.

Insert

GbetaL (MLST8 Human)

Tags

myc

Expression

Mammalian

Mutation

D42A

Available Since

May 11, 2005

Availability

Academic Institutions and Nonprofits only

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myc GbetaL mutant 6

Plasmid

#8480

Depositor

David Sabatini

Article

Kim et al Mol Cell 2003 Apr;11(4):895-904.

Insert

GbetaL (MLST8 Human)

Tags

myc

Expression

Mammalian

Mutation

T208D

Available Since

May 11, 2005

Availability

Academic Institutions and Nonprofits only

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pGTag NGFR EGFP nLAP -2

Plasmid

#194507

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Seibert et al MethodsX. 2022 Nov 18;9:101929. doi: 10.

Insert

NGFR-P2A-nLAP(EGFP)

Use

Bacterial cloning vector

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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