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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 273 results for: petD;

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Showing: 261 - 273 of 273 results

  3. deltaLoop-MIDAS

    Plasmid
    #171605
    Purpose
    Expresses the SNAP-tagged MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717) without the loop region (a.a. 4458-4496) in bacteria.
    Depositor
    Tarun Kapoor
    Article
    Mickolajczyk et al Proc Natl Acad Sci U S A. 2020 Aug 4;117
    Insert
    MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717)
    Tags
    His6 tag and SNAP tag
    Expression
    Bacterial
    Mutation
    Replaced amino acids 4458-4496 with ASGSGS linker
    Available Since
    July 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    171605-plasmid-map-sequence-id-337861

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  7. pET-Duet-6xHis-TEV-Nsp8(76-198)

    Plasmid
    #201024
    Purpose
    Bacterial expression of codon optimized SARS-CoV-2 nsp8 residues 76-198, with an N-terminus His tag followed by a TEV cleavage site.
    Depositor
    Sharon Rozovsky
    Article
    Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d
    Insert
    non-structural protein 8 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available Since
    May 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    201024-plasmid-map-sequence-id-394447

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  12. 6xHis-TEV-mCherry-LC3B-Gly (Microtubule-associated proteins 1A/1B light chain 3B)

    Plasmid
    #169168
    Purpose
    Conjugatable form of LC3B lacking 5 C-terminal amino acids (MKLSV), with C-terminal Glycine120 exposed for lipidation reaction.
    Depositor
    Sascha Martens
    Article
    Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017
    Insert
    MAP1LC3B (MAP1LC3B Human)
    Tags
    6X Histidine Tag, TEV cleavage site, mCherry
    Expression
    Bacterial
    Promoter
    T7 lac promoter
    Available Since
    May 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169168-plasmid-map-sequence-id-332048

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Showing: 261 - 273 of 273 results