We narrowed to 7,531 results for: Ski

1. pMJ839

   Plasmid

   #39317

   Depositor

   Jennifer Doudna

   Article

   Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

   Insert

   Cas9 (NMA0631 Neisseria meningitidis)
   

   Use

   CRISPR

   Tags

   His6 and MBP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

2. tCRMod-GGSGGS7

   Plasmid

   #111761

   Purpose

   Expresses soluble Clover-mRuby2 tension sensor module with 7-repeat GGSGGS extensible domain

   Depositor

   Brenton Hoffman

   Article

   LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

   Insert

   Clover-GGSGGS7-mRuby2 fusion
   

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available Since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. tCRMod-GGSGGS5

   Plasmid

   #111760

   Purpose

   Expresses soluble Clover-mRuby2 tension sensor module with 5-repeat GGSGGS extensible domain

   Depositor

   Brenton Hoffman

   Article

   LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

   Insert

   Clover-GGSGGS5-mRuby2 fusion
   

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available Since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. HA-MBP-TEVpCS-ADGRE3 CTF-FLAG

   Plasmid

   #217691

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRE3 (ADGRE3 Human)
   

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-338

   Available Since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. HA-MBP-TEVpCS-ADGRE2 CTF-FLAG

   Plasmid

   #217690

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRE2 (ADGRE2 Human)
   

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-517

   Available Since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV.Syn-FLEX.iGABASnFR.F102G.mRuby3

   Plasmid

   #112170

   Purpose

   GABA Sensor

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

   Insert

   iGABASnFR.F102G
   

   Use

   AAV

   Tags

   mRuby3

   Mutation

   F102G

   Promoter

   Synapsin

   Available Since

   July 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSI-check2-Tab1-3'UTR

   Plasmid

   #97155

   Purpose

   For dual luciferase report assay

   Depositor

   Qing Deng

   Article

   Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

   Insert

   Tab1 3'UTR
   

   Expression

   Mammalian

   Available Since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. ptagBFP-LacI

   Plasmid

   #103839

   Purpose

   Flourescent marker for lacO arrays

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   tagBFP-LacI
   

   Expression

   Mammalian

   Mutation

   LacI: C19T (silent, L7L), T264C (silent, A88A), C…

   Promoter

   SV2

   Available Since

   Dec. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. RSRF-Luc-2mut

   Plasmid

   #31819

   Depositor

   Astar Winoto

   Article

   Woronicz et al Mol Cell Biol. 1995 Nov;15(11):6364-76.

   Insert

   Mutant MEF2-dependent promoter (-307 to -242 of Nur77/NR4A1)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   two CTATATTTAG RSRF sites mutated to CGATATTTCG

   Promoter

   mutated MEF2-dependent (-307 to -242 of Nur77/NR4…

   Available Since

   Aug. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pBS NL4-3 envFS eGFP Δnef/U3

    Plasmid

    #101346

    Purpose

    Deletion from NL4-3 nt 8911-9022 and 9088-9377. This deletes nef and U3 LTR sequences

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    NL4-3
    

    Use

    Lentiviral

    Mutation

    delta nef/LTR

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. miR-vector-RFP pCDNA

    Plasmid

    #97143

    Purpose

    for expression of miRNA and TagRFP

    Depositor

    Qing Deng

    Article

    Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

    Type

    Empty backbone

    Tags

    TagRFP

    Expression

    Mammalian

    Available Since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA3.1-Muc2D1D2D3

    Plasmid

    #155215

    Purpose

    Expresses human MUC2 residues 21-1259

    Depositor

    Deborah Fass

    Article

    Javitt et al Cell. 2020 Oct 29;183(3):717-729.e16. do

    Insert

    Muc2 D1D2D3 (MUC2 Human)
    

    Tags

    6xHistadine

    Expression

    Mammalian

    Available Since

    Sept. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pmCellFree_KA1_ZIKVC

    Plasmid

    #145339

    Purpose

    Cell-free/mammalian expression vector for Zika virus capsid protein with N-term eGFP

    Depositor

    Rob Parton

    Article

    Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

    Insert

    ZIKV Capsid
    

    Tags

    8xHis and eGFP

    Expression

    Bacterial

    Promoter

    T7- CMV/CAG

    Available Since

    April 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-CAG-miniSOG

    Plasmid

    #193913

    Purpose

    Control plasmid that expresses non-targeted miniSOG in mammalian cells

    Depositor

    Xiao Wang

    Article

    Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

    Insert

    miniSOG
    

    Use

    AAV

    Tags

    Flag tag

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmCellFree_KA2_ZIKV E

    Plasmid

    #145361

    Purpose

    Cell-free/mammalian expression vector for Zika virus Envelope protein with N-term mCherry

    Depositor

    Rob Parton

    Article

    Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

    Insert

    ZIKV Envelope
    

    Tags

    8xHis and mCherry

    Expression

    Bacterial

    Promoter

    T7- CMV/CAG

    Available Since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV.hSynap.iGABASnFR.R205A

    Plasmid

    #112162

    Purpose

    GABA Sensor

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

    Insert

    iGABASnFR.R205A
    

    Use

    AAV

    Mutation

    R205A (no GABA binding)

    Promoter

    hSynapsin

    Available Since

    July 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. GST-Cav FL (1-178)

    Plasmid

    #22445

    Depositor

    William Sessa

    Article

    Garcia-Cardena et al J Biol Chem. 1997 Oct 10. 272(41):25437-

    Insert

    Caveolin1(1-178)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Dec. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA3.0_nucLAMA-F98 IRES EGFP

    Plasmid

    #136618

    Purpose

    Plasmid encoding nuclear localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and EGFP

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    SNAP-Tag-LAMA-F98-NLS (IRES EGFP)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV.hSynap.iGABASnFR.F102Y.Y137L

    Plasmid

    #112161

    Purpose

    GABA Sensor

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

    Insert

    iGABASnFR.F102Y.Y137L
    

    Use

    AAV

    Mutation

    F102Y.Y137L

    Promoter

    hSynapsin

    Available Since

    July 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV.GFAP.iGABASnFR.F102Y.Y137L

    Plasmid

    #112174

    Purpose

    GABA Sensor

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

    Insert

    iGABASnFR.F102Y.Y137L
    

    Use

    AAV

    Mutation

    F102Y.Y137L

    Promoter

    GFAP

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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