We narrowed to 11,368 results for: Vars;

1. pEY60

   Plasmid

   #191057

   Purpose

   acc-2 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

   Depositor

   Oliver Hobert, Eviatar Yemini

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   acc-2 (acc-2 Nematode)
   

   Expression

   Worm

   Mutation

   None

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEY70

   Plasmid

   #191067

   Purpose

   ceh-48 fluorescent neural reporter driving nuclear TagRFP-T expression (refer to NeuroPAL paper for expression)

   Depositor

   Oliver Hobert, Eviatar Yemini

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   ceh-48 (ceh-48 Nematode)
   

   Expression

   Worm

   Mutation

   None

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEY66

   Plasmid

   #191063

   Purpose

   C15C8.4 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

   Depositor

   Oliver Hobert, Eviatar Yemini

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   C15C8.4 (C15C8.4 Nematode)
   

   Expression

   Worm

   Mutation

   None

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEY77

   Plasmid

   #191072

   Purpose

   dop-3 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

   Depositor

   Oliver Hobert, Eviatar Yemini

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   dop-3 (dop-3 Nematode)
   

   Expression

   Worm

   Mutation

   None

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEY81

   Plasmid

   #191076

   Purpose

   flp-32 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

   Depositor

   Oliver Hobert, Eviatar Yemini

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   flp-32 (flp-32 Nematode)
   

   Expression

   Worm

   Mutation

   None

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEY75

   Plasmid

   #191071

   Purpose

   cng-3 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

   Depositor

   Oliver Hobert, Eviatar Yemini

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   cng-3 (cng-3 Nematode)
   

   Expression

   Worm

   Mutation

   None

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEY67

   Plasmid

   #191064

   Purpose

   ceh-1 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

   Depositor

   Oliver Hobert, Eviatar Yemini

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   ceh-1 (ceh-1 Nematode)
   

   Expression

   Worm

   Mutation

   None

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEY69

   Plasmid

   #191066

   Purpose

   ceh-24 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

   Depositor

   Oliver Hobert, Eviatar Yemini

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   ceh-24 (ceh-24 Nematode)
   

   Expression

   Worm

   Mutation

   None

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pNHT7

   Plasmid

   #186753

   Purpose

   A basic cloning vector for inserting genes with 5' and 3' UTRs for later subcloning via Golden Gate assembly.

   Depositor

   Bo Wang

   Article

   Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

   Type

   Empty backbone

   Use

   Synthetic Biology; Cloning backbone

   Promoter

   T7

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. MBP_Lims (MBP_LIM1234)

    Plasmid

    #182042

    Purpose

    For expression of human paxillin LIM domains 1-4 with N-terminal GST fusion protein in E. coli.

    Depositor

    Vesa Hytönen

    Article

    Ripamonti et al Commun Biol. 2021 Mar 29;4(1):380. doi:

    Insert

    LIM1234 (PXN Human)
    

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    LIM domains 1-4 only

    Available Since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. GST_LIM234

    Plasmid

    #182039

    Purpose

    For expression of human paxillin LIM domains 2-4 with N-terminal GST fusion protein in E. coli.

    Depositor

    Vesa Hytönen

    Article

    Ripamonti et al Commun Biol. 2021 Mar 29;4(1):380. doi:

    Insert

    LIM234 (PXN Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    LIM domains 2-4 only

    Available Since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pT7-RiboJ-mTFP1-T7

    Plasmid

    #173225

    Purpose

    Encodes mTFP fluorescent protein. To be used in cell-free system

    Depositor

    Jim Haseloff

    Article

    Guzman-Chavez et al ACS Synth Biol. 2022 Mar 18;11(3):1114-1

    Insert

    mTFP1
    

    Use

    Cell-free system

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFGC-T7RibJ-RRvT

    Plasmid

    #173226

    Purpose

    Encodes RRvT fluorescent protein. To be used in cell-free system

    Depositor

    Jim Haseloff

    Article

    Guzman-Chavez et al ACS Synth Biol. 2022 Mar 18;11(3):1114-1

    Insert

    RRvT
    

    Use

    Cell-free system

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 9, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pDSARN-vasa-eSpCas9sv40

    Plasmid

    #173670

    Purpose

    eSpCas9 expression vector for Anopheles

    Depositor

    Eric Marois

    Article

    Klug et al PLoS Pathog. 2022 Oct 12;18(10):e1010881

    Insert

    eSpCas9 with Anopheles vasa promoter and SV40 terminator
    

    Use

    CRISPR

    Expression

    Insect

    Mutation

    mutations in Cas9 reducing off-target activity (S…

    Promoter

    vasa

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1-Flag-stm2585

    Plasmid

    #174391

    Purpose

    S. Typhimurium gene stm2585 (sarA/steE) codon-optimized for mammalian expression with N-terminal Flag tag

    Depositor

    Dennis Ko

    Article

    Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

    Insert

    stm2585
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-GFAP104-ChromeT-mCherry

    Plasmid

    #123126

    Purpose

    AAV-mediated expression of ChromeT-mCherry under the GFAP promoter.

    Depositor

    Edward Boyden

    Article

    Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

    Insert

    ChromeT-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Channelrhodopsin-2 (ChR2) with mutations A71S/ E9…

    Promoter

    GFAP104

    Available Since

    April 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCFJ1972 - [1-2] ENTR - Flour - TagBFP2(NLS(2), no_atg, no_stop)

    Plasmid

    #159844

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Flour - TagBFP2(NLS(2), no_atg, no_stop)
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCFJ2301 - [1-2] ENTR - Fluor - ce-mMaple3(1 intron, syntrons(3), no_atg, no_stop)

    Plasmid

    #159865

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-mMaple3(1 intron, syntrons(3), no_atg, no_stop)
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. sg2

    Plasmid

    #113967

    Purpose

    Single short guide RNA targeting TACCACATTTGTAGAGGTT

    Depositor

    Jordan Green

    Article

    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

    Insert

    sg2
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. sg3

    Plasmid

    #113968

    Purpose

    Single short guide RNA targeting CAATGTATCTTATCATGTC

    Depositor

    Jordan Green

    Article

    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

    Insert

    sg3
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. sg2+sg3

    Plasmid

    #113970

    Purpose

    Double short guide RNA targeting TACCACATTTGTAGAGGTT & CAATGTATCTTATCATGTC

    Depositor

    Jordan Green

    Article

    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

    Insert

    sg2+sg3
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCDNA3 mSmoothened NQ4

    Plasmid

    #126407

    Purpose

    Expression of Smoothened NQ4 in mammalian cell lines.

    Depositor

    Stacey Ogden

    Article

    Marada et al PLoS Genet. 2015 Aug 20;11(8):e1005473.

    Insert

    m-Smoothened (Smo Mouse)
    

    Expression

    Mammalian

    Mutation

    Smo glycosylation site NQ4

    Available Since

    June 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCDNA3 mSmoothened NQ3

    Plasmid

    #126406

    Purpose

    Expression of Smoothened NQ3 in mammalian cell lines.

    Depositor

    Stacey Ogden

    Article

    Marada et al PLoS Genet. 2015 Aug 20;11(8):e1005473.

    Insert

    m-Smoothened (Smo Mouse)
    

    Expression

    Mammalian

    Mutation

    Smo glycosylation site NQ3

    Available Since

    June 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSLQ1371-sgYAP-10

    Plasmid

    #121424

    Purpose

    sgYAP-10 sequence: GTGCTGTCCCAGATGAACGTC. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

    Depositor

    Ovijit Chaudhuri, Stanley Qi

    Article

    Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:

    Insert

    sgYAP-10 (GTGCTGTCCCAGATGAACGTC)
    

    Use

    CRISPR and Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    mouse U6

    Available Since

    April 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCDB367

    Plasmid

    #110277

    Purpose

    gHEEE_02 with a N-terminal 10-His DsbC TEV-peptide fusion

    Depositor

    Christopher Bahl

    Article

    Buchko et al Protein Sci. 2018 Aug 26. doi: 10.1002/p

    Insert

    gHEEE_02
    

    Tags

    10-His, Tev recognition peptide, and mature DsbC …

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLenti CRISPR V2 sgMTHFD1L_2

    Plasmid

    #106317

    Purpose

    Express Cas9 and sgRNA targeting MTHFD1L

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    sgRNA targeting MTHFD1L
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHH0103_TJP3-1/1

    Plasmid

    #91545

    Purpose

    Protein expression and purification of human SH3 domain construct TJP3-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    TJP3-1/1 (TJP3 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHH0103_RAB4B-2/2

    Plasmid

    #91554

    Purpose

    Protein expression and purification of human SH3 domain construct RAB4B-2/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    RAB4B-2/2 (RAB4B Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHH0103_NCF4-1/1

    Plasmid

    #91561

    Purpose

    Protein expression and purification of human SH3 domain construct NCF4-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    NCF4-1/1 (NCF4 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHH0103_MPP5-1/1

    Plasmid

    #91562

    Purpose

    Protein expression and purification of human SH3 domain construct MPP5-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    MPP5-1/1 (PALS1 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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