We narrowed to 7,237 results for: ALP
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Plasmid#69586PurposeExpresses mutated p53-L344P tagged with mKate2 and split N-term mVenusDepositorInsertp53-L344P (TP53 Human)
UseLentiviralTagsmKate2 and split mVenus - N termExpressionMammalianMutationp53 L344P mutation that prevents dimerization and…PromoterEF1alphaAvailable sinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0 mCherry_K-RasC14
Plasmid#101286PurposeLentiviral expression of K-Ras C14DepositorInsertK-Ras (KRAS Human)
UseLentiviralTagsmCherryExpressionMammalianMutationC14PromoterAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_Y357F-PolyA
Plasmid#112287PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) Y357F mutant _ corresponding to tyrosine 407 in this isoformDepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationTyrosine 357 to Phenyalanine corresponding to tyr…PromoterEF-1 alphaAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-Duet-ECHA(37-763)-FLAG (no His-tag)
Plasmid#96887Purposeexpresses flag-tagged mouse ECHA (37-768) in E.coli cells (no His-tag)DepositorInsertTrifunctional enzyme subunit alpha, mitochondrial (Hadha Mouse)
UseTagsFLAG-taggedExpressionBacterialMutationdeleted amino acid 1-36PromoterT7Available sinceJuly 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0 shCavin1KDR-EGFP
Plasmid#187254PurposeLentiviral expression of shRNA targeting Cavin1 + reconstitution of mouse Cavin1 (Cavin1 rescue)DepositorInsertShRNA for Cavin1 (Cavin1 Mouse)
UseLentiviralTagsEGFPExpressionMutationPromoterU6, 5'LTRAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0_shRNAHsCavin1_Del4UC1_EGFP
Plasmid#229691PurposeLentiviral expression of shRNA targeting Cavin1 + reconstitution of zebrafish mutant Cavin1 (Del4UC1)DepositorUseLentiviralTagsEGFPExpressionMutationDel4UC1PromoterLTR viral promoterAvailable sinceJan. 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
AAV-CMV-scFVDnmt3A_bGHpA
Plasmid#177349PurposeAAV expression of scFV-fused catalytic domain of Dnmt3a for targeted DNA methylation editingDepositorInsertcatalytic domain of DNA Methyltransferase 3 Alpha (Dnmt3a Mouse)
UseAAVTagsmyc and scFVExpressionMammalianMutation598–908 aa of mouse Dnmt3aPromoterCMVAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK1306-AAV-EFSNC-dSaCas9-HP1aNH(115-177)
Plasmid#223151PurposeExpression of truncated HP1a with dSaCas9 and empty gRNA scaffoldDepositorInsertHP1a (CBX5 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 115-177PromoterEF1aAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK1296-AAV-EFSNC-dCjCas9-HP1aNH(115-177)
Plasmid#223141PurposeExpression of truncated HP1a with dCjCas9 and empty gRNA scaffoldDepositorInsertHP1a (CBX5 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 115-177PromoterEF1aAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
PLL5.0-Anillin ShRNA-Cherry-Anillin deltaAnillin homology domain
Plasmid#187277PurposeLentiviral expression of shRNA targeting Anillin + reconstitution of Anillin delta Anillin homology domainDepositorInsertAnillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
UseLentiviralTagsmCherryExpressionMutationdeltaAnillin homology domainPromoterU6, CMVAvailable sinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
An R57A, R59A CAAX pMT2
Plasmid#206119PurposeCav2.1 N-terminus (amino acids 1-100) with R57A and R59A mutations that prevent dominant-negative suppression of Cav2.1 currents and a C-terminal CAAX (last 10 aa of H.Ras) motifDepositorInsertcacna1a (Cacna1a Rat)
UseTagsExpressionMammalianMutationR57A, R59APromoterAd MLP/TPL/SV40Available sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_PPP2CA_CDKL3
Plasmid#205900PurposeExpress mEGFP-tagged fusion protein, PPP2CA_CDKL3 from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_PAX5_ESRRA
Plasmid#205880PurposeExpress mEGFP-tagged fusion protein, PAX5_ESRRA from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
An R57A, R59A HA CAAX pMT2
Plasmid#206126PurposeCav2.1 N-terminus (amino acids 1-100) with R57A and R59A mutations that prevent dominant-negative suppression of Cav2.1 currents and a C-terminal CAAX (last 10 aa of H.Ras) motif and a HA tagDepositorInsertcacna1a (Cacna1a Rat)
UseTagsExpressionMammalianMutationR57A, R59APromoterAd MLP/TPL/SV40Available sinceOct. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 HA EIVA (E1658A) pCAGGS
Plasmid#206082Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation in the P loop of Domain IV which reduces trafficking to the cell surfaceDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationE1658APromoterCMV/B-actinAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 HA EIA (E314A) pCAGGS
Plasmid#206081Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation in the P loop of Domain I which reduces trafficking to the cell surfaceDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationE314APromoterCMV/B-actinAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 HA EIVK (E1658K) pcDNA3
Plasmid#206066Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658K mutation in the P loop of Domain IV which abolishes trafficking to the cell surfaceDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationE1658KPromoterCMVAvailable sinceOct. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 HA EIVK (E1658K) pCAGGS
Plasmid#206096Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658K mutation in the P loop of Domain IV which abolishes trafficking to the cell surfaceDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationE1658KPromoterCMV/B-actinAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT2/shCol1a1/Scarlet_Seq1.3
Plasmid#206137PurposeKnockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.DepositorInsertCOL1A1 (Col1a1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT2/shCol1a1/GFP4_Seq1.3
Plasmid#206136PurposeKnockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.DepositorInsertCOL1A1 (Col1a1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1101
Plasmid#143925PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertGTF2IRD1 (GTF2IRD1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1100
Plasmid#143924PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertGTF2IRD1 (GTF2IRD1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-FCAR-COMP5AP-AviTag-9xHis
Plasmid#157183PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertFCAR (FCAR Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-TNFRSF19-COMP5AP-AviTag-9xHis
Plasmid#157098PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertTNFRSF19 (TNFRSF19 Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-ULBP2-COMP5AP-AviTag-9xHis
Plasmid#157160PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertULBP2 (ULBP2 Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmCherry2-GNB1-T2A-mCherry2-GNG2-IRES-GNAI1-mEYFP(Q69K)
Plasmid#190755PurposeExpression of trimeric G protein with mCherry2 (GNB1 and GNG2) or mEYFP(Q69K) (GNAI1) tags.DepositorUseTagsmCherry2 and mEYFP(Q69K)ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
PLL5.0-Anillin ShRNA-GFP-AnillinA740D, E758K
Plasmid#187274PurposeLentiviral expression of shRNA targeting Anillin + reconstitution of Anillin A740D,E758KDepositorInsertAnillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
UseLentiviralTagsEGFPExpressionMutationAnillin A740D, E758KPromoterU6, 5'LTRAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
PMP34-hLOV-TEVcs-GAL4bd-VP64-V5
Plasmid#170997PurposeHiLITR transcription factor with full-length PMP34 targeting sequence (Peroxisome)DepositorInsertPMP34(FL)-NNES-hLOV-TEVcs(ENLYFQ-M)-GAL4bd-VP64-V5 (SLC25A17 Synthetic)
UseLentiviral and Synthetic BiologyTagsV5ExpressionMammalianMutationPromoterEf1-alphaAvailable sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
Venus-Puma-4E-pEGFP-C1
Plasmid#166746PurposeExpress Venus fused to the N-terminus of the Bcl-2 family protein, Puma with mutation in BH3 domain to disrupt binding anti-apoptotic proteinsDepositorInsertPuma-4E (BBC3 Human)
UseTagsVenusExpressionMammalianMutation4 hydrophobic residues in the BH3 region mutated …PromoterCMVAvailable sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX307_PDHA2
Plasmid#115192PurposeLentiviral transduction and expression of PDHA2 into any mammalian cellDepositorInsertPDHA2WT (PDHA2 Human)
UseLentiviralTagsExpressionMutationPromoterEF1alphaAvailable sinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX307_PDHA2S291A
Plasmid#115196PurposeLentiviral transduction and expression of PDHA2S291A into any mammalian cellDepositorInsertPDHA2S291A (PDHA2 Human)
UseLentiviralTagsExpressionMutationp.S291APromoterEF1alphaAvailable sinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX307_PDHA2S291A/S293A
Plasmid#115198PurposeLentiviral transduction and expression of PDHA2S291A/S293A into any mammalian cellDepositorInsertPDHA2S291A/S293A (PDHA2 Human)
UseLentiviralTagsExpressionMutationp.S291A/S293APromoterEF1alphaAvailable sinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-3 SIRT1-NTerm Exon1-Exon3
Plasmid#105681PurposeBacterial Expression construct of N terminus of SIRT1: Exon1-Exon3 (deltaExon2)DepositorInsertSIRT1-NTerm: Exon1-Exon3 (Sirt1 Mouse)
UseTagsGSTExpressionBacterialMutationSynonymous base changes not affecting the protein…Promotertac_PromoterAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-3- SIRT1-NTerm Exon1-Exon2-Exon3
Plasmid#105680PurposeBacterial Expression construct of N terminus of SIRT1: Exon1-Exon2-Exon3DepositorInsertSIRT1-NTerm: Exon1-Exon2-Exon3 (Sirt1 Mouse)
UseTagsGSTExpressionBacterialMutationSynonymous base changes not affecting the protein…Promotertac_PromoterAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-shDnd1-No1
Plasmid#70058PurposeTRC lentiviral vector for shRNA against mouse Dnd1 #1DepositorInsertDnd1 (Dnd1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-shDnd1-No3
Plasmid#70060PurposeTRC lentiviral vector for shRNA against mouse Dnd1 #3DepositorInsertDnd1 (Dnd1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-shDnd1-No4
Plasmid#70065PurposeTRC lentiviral vector for shRNA against mouse Dnd1 #4DepositorInsertDnd1 (Dnd1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-shDnd1-No5
Plasmid#70066PurposeTRC lentiviral vector for shRNA against mouse Dnd1 #5DepositorInsertDnd1 (Dnd1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-shDnd1-No6
Plasmid#70067PurposeTRC lentiviral vector for shRNA against mouse Dnd1 #6DepositorInsertDnd1 (Dnd1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-shDnd1-No8
Plasmid#70069PurposeTRC lentiviral vector for shRNA against mouse Dnd1 #8DepositorInsertDnd1 (Dnd1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_TNF_p.S157R
Plasmid#81344PurposeGateway Donor vector containing TNF , part of the Target Accelerator Plasmid Collection.DepositorInsertTNF (TNF Human)
UseGateway entry vectorTagsExpressionMutationS157RPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
MAPK14 gRNA (BRDN0001487162)
Plasmid#77925Purpose3rd generation lentiviral gRNA plasmid targeting human MAPK14DepositorInsertMAPK14 (MAPK14 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
CSNK2A2 gRNA (BRDN0001144724)
Plasmid#75586Purpose3rd generation lentiviral gRNA plasmid targeting human CSNK2A2DepositorInsertCSNK2A2 (CSNK2A2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
CSNK2A2 gRNA (BRDN0001147190)
Plasmid#75587Purpose3rd generation lentiviral gRNA plasmid targeting human CSNK2A2DepositorInsertCSNK2A2 (CSNK2A2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
CSNK2A2 gRNA (BRDN0001148502)
Plasmid#75588Purpose3rd generation lentiviral gRNA plasmid targeting human CSNK2A2DepositorInsertCSNK2A2 (CSNK2A2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRRLNeo-pEF1a-p53ashL344A-mKate2-splitmVenusN
Plasmid#69584PurposeExpresses mutated p53-L344A tagged with mKate2 and split N-term mVenusDepositorInsertp53-L344A (TP53 Human)
UseLentiviralTagsmKate2 and split mVenus - N termExpressionMammalianMutationp53 L344A mutation that prevents tetramerization …PromoterEF1alphaAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
mBFP-APP-mGFP
Plasmid#196694PurposeExpresses APP695 with two fluorescent proteins at the ends to follow processing in mammalian cellsDepositorInsertAmyloid Precursor Protein 695 isoform (APP Human)
UseTagsmEGFP and mtagBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLX313-TP53-WT
Plasmid#118014PurposeConstitutive expression of wild-type human Tumor Protein p53 (TP53)DepositorInsertTP53 (TP53 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1alphaAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCVneo-HA-ER-Hoxb8
Plasmid#222291PurposeThe most commonly used plasmid for the generation of ER-Hoxb8 conditionally immortalized myeloid cell lines.DepositorUseRetroviralTagsHAExpressionMammalianMutationThe estrogen receptor hormone binding domain cont…PromoterAvailable sinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-4:TUBA1B-mEGFP
Plasmid#87421PurposeHomology arms and linker-mEGFP sequence for N-terminus (second exon) tagging of human TUBA1BDepositorInsertTUBA1B Homology Arms with mEGFP-linker (TUBA1B Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceMarch 15, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits