We narrowed to 5,474 results for: chia

1. pBN262

   Plasmid

   #167967

   Purpose

   express E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E
   

   Use

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   Y708E, Y713E, Y715E, Y717E

   Promoter

   araBAD

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBRC901

   Plasmid

   #167948

   Purpose

   express wild-type bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30)

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   wild-type bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30)
   

   Use

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBN202

   Plasmid

   #167950

   Purpose

   express E. coli E69 (O9a:K30) Wzc_K540M with Y713E, Y715E, Y717E, Y718E mutations

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   E. coli E69 (O9a:K30) Wzc_K540M with Y713E, Y715E, Y717E, Y718E mutations
   

   Use

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   K540M, Y713E, Y715E, Y717E, Y718E

   Promoter

   araBAD

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. 4LNDD-pCDNA3.1(+)

   Plasmid

   #172227

   Purpose

   Expresses human ADAR2 Deaminase Domain (DD) fused with 4 repeats of lambda N peptide (4LN) in mammalian cells

   Depositor

   Joshua Rosenthal

   Article

   Montiel-Gonzalez et al Nucleic Acids Res. 2016 Dec 1;44(21):e15

   Insert

   Lambda N peptide - human ADAR2 Deaminase Domain
   

   Use

   Tags

   6xHis and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKP1693

   Plasmid

   #167595

   Purpose

   Express FepA with His6 tag between residues 393 and 394 of the mature amino acid sequence . Please note that mutation numbers are not based on start codon of insert.

   Depositor

   Kathleen Postle

   Article

   Gresock et al J Bacteriol. 2017 Apr 25;199(10). pii: J

   Insert

   FepA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   His6 tag inserted after residue 393 in the mature…

   Promoter

   Available since

   July 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-T7-null (BPK1077)

   Plasmid

   #171128

   Purpose

   Plasmid encoding a pCMV-T7 with a 2 amino acid peptide prior to a stop codon.

   Depositor

   Benjamin Kleinstiver

   Article

   Johnson et al Mol Cell. 2022 Jun 2;82(11):2161-2166.e3

   Insert

   pCMV-T7 null expression plasmid that encodes a two amino acid peptide (Met-Asp)
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   pCMV and T7

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKB238

   Plasmid

   #170033

   Purpose

   Encodes LacI-controlled expression of surface-displayed NPY and PvirK-mNeonGreen reporter

   Depositor

   Jeffrey Tabor

   Article

   Brink et al bioRxiv

   Inserts

   mNeonGreen
   Neuropeptide Y (NPY Synthetic, Human, Escherichia coli)
   

   Use

   Synthetic Biology

   Tags

   Lpp, OmpA, and Tether

   Expression

   Bacterial

   Mutation

   Promoter

   Ptac and PvirK

   Available since

   July 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKB235

   Plasmid

   #170024

   Purpose

   Encodes LacI-controlled expression of surface-displayed CCL20 and PvirK-mNeonGreen reporter

   Depositor

   Jeffrey Tabor

   Article

   Brink et al bioRxiv

   Inserts

   mNeonGreen
   CCL20 (CCL20 Synthetic, Human, Escherichia coli)
   

   Use

   Synthetic Biology

   Tags

   Lpp, OmpA, and Tether

   Expression

   Bacterial

   Mutation

   Promoter

   Ptac and PvirK

   Available since

   July 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. AlkB-D135G

   Plasmid

   #160434

   Purpose

   A plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to Gly

   Depositor

   Tao Pan

   Article

   Wang et al Nucleic Acids Res. 2021 Mar 18;49(5):e30

   Insert

   E. coli AlkB
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Changed Aspartic acid 135 to Glycine; deleted the…

   Promoter

   T7

   Available since

   Dec. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDF-NtXNtS1HR1AtR2NtB2

    Plasmid

    #160890

    Purpose

    Expression of N. tabacum Rubisco small subunit (rbcS-SH1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.

    Depositor

    Maureen Hanson

    Article

    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

    Insert

    PT7-Nt-rbcS-S1H
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    rbcS-SH1: T7, chaperones: T13

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDF-NtXNtT4aR1AtR2NtB2

    Plasmid

    #160887

    Purpose

    Expression of N. tabacum Rubisco small subunit (rbcS-T4a), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.

    Depositor

    Maureen Hanson

    Article

    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

    Insert

    PT7-Nt-rbcS-T4a
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rbcS-T4a: T7, chaperones: T10

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. SMT215

    Plasmid

    #134818

    Purpose

    SMT205 with DHFR-FLAG-tag, western blot

    Depositor

    Tanja Kortemme

    Article

    Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1

    Inserts

    Dihydrofolate reductase
    Thymidylate synthase
    

    Use

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    Silent mutations to eliminate internal restrictio…

    Promoter

    T7 and TET

    Available since

    Oct. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. SMT101

    Plasmid

    #134815

    Purpose

    Dual expression of DHFR and TYMS, in vivo assays

    Depositor

    Tanja Kortemme

    Article

    Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1

    Inserts

    Dihydrofolate reductase
    Thymidylate synthase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Silent mutations to eliminate internal restrictio…

    Promoter

    T7

    Available since

    Oct. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET15-His-∆NGlpG(Y205F)

    Plasmid

    #155132

    Purpose

    Expresses N-terminally His-tagged E. coli GlpG Y205F variant (residues 87-276) from pET15b

    Depositor

    Sinisa Urban

    Article

    Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

    Insert

    GlpG (glpG Escherichia coli)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Y205F, insert codes for GlpG residues 87-276

    Promoter

    T7

    Available since

    Sept. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. SMT201

    Plasmid

    #134816

    Purpose

    SMT101 with TET promoter for TYMS, in vivo assays

    Depositor

    Tanja Kortemme

    Article

    Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1

    Inserts

    Dihydrofolate reductase
    Thymidylate synthase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Silent mutations to eliminate internal restrictio…

    Promoter

    T7 and TET

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pC0.007

    Plasmid

    #119671

    Purpose

    Level 0 part. Promoter, cpc560 promoter, extended spacer region, downstream TFB site region duplicated.

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Insert

    Pcpc560-Dx2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    bp 89 T to C, bp 187 C to A, Duplicated downstrea…

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pC0.006

    Plasmid

    #119670

    Purpose

    Level 0 part. Promoter, cpc560 promoter, extended spacer region, upstream TFB site region duplicated.

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Insert

    Pcpc560-Ux2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    bp 89 T to C, bp 187 C to A, Duplicated upstream …

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMH-HT_ygiF-TTM

    Plasmid

    #127794

    Purpose

    Expresses the TTM/CYTH domain of ygiF in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    ygiF (ygiF Escherichia coli)
    

    Use

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    N-terminal TTM domain of ygiF (residues 1-200)

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNIC28-BSA4-S15

    Plasmid

    #127821

    Purpose

    Production of recombinant human ribosomal protein S15

    Depositor

    Ivanhoe Leung

    Article

    Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

    Insert

    RPS15 (RPS15 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNH-TrxT-G-T136D-S15

    Plasmid

    #127824

    Purpose

    Production of recombinant human ribosomal protein S15

    Depositor

    Ivanhoe Leung

    Article

    Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

    Insert

    RPS15 (RPS15 Human)
    

    Use

    Tags

    His6 - Thioredoxin - TEV

    Expression

    Bacterial

    Mutation

    T136D

    Promoter

    T7

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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