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Showing: 5601 - 5640 of 8357 results

  1. TH mAb_BCN53.1.1D10_LC_Rat Ig Kappa

    Plasmid
    #182899
    Purpose
    Mammalian expression of TH rat mAb LC gene
    Depositor
    Heng Zhu
    Insert
    anti-TH (human) light chain (TH Rat)
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    No
    Promoter
    CMV
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    182899-plasmid-map-sequence-id-360595

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  2. EP300

    Plasmid
    #39018
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    EP300 (EP300 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  3. pALS3-Ma-sfGFP-WT

    Plasmid
    #212119
    Purpose
    Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.
    Depositor
    Ryan Mehl
    Inserts
    SUMO-sfGFP-WT
    M. alvus Pyl-tRNA(6)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    araC and lpp
    Available since
    Sept. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    212119-plasmid-map-sequence-id-444185

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  4. KRigor980

    Plasmid
    #129763
    Purpose
    Expresses full-length Drosophila Kinesin-1 with rigor mutation (R210A) for adhering microtubules to surfaces
    Depositor
    William Hancock
    Insert
    KHC (Khc Fly)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    R210A
    Promoter
    Available since
    Nov. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    129763-plasmid-map-sequence-id-263579

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  5. INCENP

    Plasmid
    #39185
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    INCENP (INCENP Human)
    Use
    Tags
    GST-Tev
    Expression
    Bacterial
    Mutation
    Complex with AURKBA-c004
    Promoter
    Available since
    June 7, 2013
    Availability
    Academic Institutions and Nonprofits only

  6. pLEX307-ACE2-G418

    Plasmid
    #158450
    Purpose
    Constitutive lentiviral expression of human ACE2.
    Depositor
    Sho Iketani, Alejandro Chavez
    Insert
    ACE2 (ACE2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158450-plasmid-map-sequence-id-308176

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  7. KIF3A560GFP

    Plasmid
    #129769
    Purpose
    Expresses mouse Kinesin-2 KIF3A head and neck linker, dimerized through kinesin-1 neck-coil and coil-1 to 560 and GFP tagged
    Depositor
    William Hancock
    Insert
    KIF3A (Kif3a Mouse)
    Use
    Tags
    GFP and His6
    Expression
    Bacterial
    Mutation
    Truncation at 359, fused to kinesin-1 starting Al…
    Promoter
    Available since
    Nov. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    129769-plasmid-map-sequence-id-263530

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  8. pKG96

    Plasmid
    #63072
    Purpose
    Retroviral expression of EGFP-TEV-S-tag CENP-A for protein localization and affinity purification (LAP)
    Depositor
    Iain Cheeseman
    Insert
    CENP-A (CENPA Human)
    Use
    Retroviral
    Tags
    EGFP and TEV-S
    Expression
    Mammalian
    Mutation
    A12V, C75Y, and L121F mutations compared to GenBa…
    Promoter
    Available since
    Jan. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    63072-plasmid-map-sequence-id-134723

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  9. BRD1

    Plasmid
    #39095
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    BRD1 (BRD1 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  10. pLPC-C-SF-TAP-DEST-TLK1-WT

    Plasmid
    #218829
    Purpose
    Expresses C-terminal Strep-FLAG-tagged wild type human TLK1 variant 5 in a retrovirus vector.
    Depositor
    Travis Stracker
    Insert
    TLK1 (TLK1 Human)
    Use
    Tags
    FLAG-STREP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    218829-plasmid-map-sequence-id-436653

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  11. pGEX6P1-DEST-FLAG

    Plasmid
    #119754
    Purpose
    Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal FLAG tag.
    Depositor
    Andrew Jackson, Martin Reijns
    Type
    Empty backbone
    Use
    Tags
    FLAG and GST
    Expression
    Bacterial
    Mutation
    Promoter
    tac promoter
    Available since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    119754-plasmid-map-sequence-id-234028

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  12. CECR2

    Plasmid
    #39066
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CECR2 (CECR2 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  13. OptoEphB2KD-mCherry

    Plasmid
    #92044
    Purpose
    Expresses kinase-dead mutant of OptoEphB2 labeled with mCherry
    Depositor
    Ji Yu
    Insert
    EPHB2 (EPHB2, CRY2 Mustard Weed, Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    92044-plasmid-map-sequence-id-182373

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  14. K560_AviC_coBirA

    Plasmid
    #129765
    Purpose
    Expresses Drosophila Kinesin-1 truncated at 560 and biotinylated at tail for single-molecule assays
    Depositor
    William Hancock
    Insert
    KHC (Khc Fly)
    Use
    Tags
    Avi-tag GLNDIFEAQKIEWH and His6
    Expression
    Bacterial
    Mutation
    Truncation at 559
    Promoter
    Available since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    129765-plasmid-map-sequence-id-263583

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  15. ACAA1

    Plasmid
    #38847
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ACAA1 (ACAA1 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  16. AAV pCAG-FLEX-mRuby3-WPRE

    Plasmid
    #99279
    Purpose
    Can be used to generate AAV virus that will express mRuby3 in the presence of Cre
    Depositor
    Rylan Larsen
    Insert
    mRuby3
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    99279-plasmid-map-sequence-id-181812

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  17. p2Bac.His.XPD

    Plasmid
    #39217
    Depositor
    Aziz Sancar
    Insert
    XPD (ERCC2 Human)
    Use
    Tags
    His (HHYHHH)
    Expression
    Insect
    Mutation
    XPD starts at aa4
    Promoter
    Pph
    Available since
    Aug. 24, 2012
    Availability
    Academic Institutions and Nonprofits only

  18. pLenti-EF1α-IRES-EGFP-hMCIDAS

    Plasmid
    #149696
    Purpose
    Expresses untagged human MCIDAS in mammalian cells
    Depositor
    Ken-Ichi Takemaru
    Insert
    MCIDAS (MCIDAS Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    EF1 alpha
    Available since
    July 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    149696-plasmid-map-sequence-id-306847

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  19. p2Bac.His.XPB

    Plasmid
    #39216
    Depositor
    Aziz Sancar
    Insert
    XPB (ERCC3 Human)
    Use
    Tags
    His
    Expression
    Insect
    Mutation
    Promoter
    Pph
    Available since
    Aug. 24, 2012
    Availability
    Academic Institutions and Nonprofits only

  22. MAT1A

    Plasmid
    #38852
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MAT1A (Mat1a Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  23. TH mAb_BCN53.1.1D10_LC_Mouse Ig Kappa

    Plasmid
    #182897
    Purpose
    Mammalian expression of TH mouse mAb LC gene
    Depositor
    Heng Zhu
    Insert
    anti-TH (human) light chain (TH Mouse)
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    No
    Promoter
    CMV
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    182897-plasmid-map-sequence-id-360632

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  24. psd44-G13WT

    Plasmid
    #46829
    Purpose
    expression of wild type G protein alpha 13 in mammalian cells
    Depositor
    Agnese Mariotti
    Insert
    GNA13 (GNA13 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PGK promoter
    Available since
    July 30, 2013
    Availability
    Academic Institutions and Nonprofits only

  25. DN2-Gli3 in pECE

    Plasmid
    #121971
    Purpose
    medium Gli3 transcriptional activator
    Depositor
    James Briscoe
    Insert
    DN2Gli3 (GLI3 Human)
    Use
    Chick electroporation
    Tags
    5xmyc
    Expression
    Mammalian
    Mutation
    N terminus deletion (nt1-1401)
    Promoter
    Available since
    April 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    121971-plasmid-map-sequence-id-241250

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  26. HGS

    Plasmid
    #39101
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    HGS (HGS Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  27. DYRK2

    Plasmid
    #39031
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    DYRK2 (DYRK2 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    39031-plasmid-map-sequence-id-314867

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  28. pFRT-DestFLAGHA_HuC

    Plasmid
    #65759
    Purpose
    constitutively expresses FLAG-HA-tagged HuC in mammalian cells
    Depositor
    Thomas Tuschl
    Insert
    ELAVL3 (ELAVL3 Human)
    Use
    Tags
    FLAG HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, 2x Tet
    Available since
    June 4, 2015
    Availability
    Academic Institutions and Nonprofits only

  29. pBJ-FLAG-hPar4

    Plasmid
    #53231
    Purpose
    mammalian expression of Flag-tagged human Par4
    Depositor
    Shaun Coughlin
    Insert
    Par4 (F2RL3 Human)
    Use
    Tags
    FLAG and bovine prolactin signal sequence (MDSKGS…
    Expression
    Mammalian
    Mutation
    Deletion of aa1-24 (native signal peptide)
    Promoter
    Available since
    Aug. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    53231-plasmid-map-sequence-id-176053

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  30. ATAD2

    Plasmid
    #38916
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ATAD2 (ATAD2 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    38916-plasmid-map-sequence-id-151434

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  31. pET28-6His-SUMO-Nsp14

    Plasmid
    #154956
    Purpose
    Expresses Nsp14 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli
    Depositor
    Justin Kollman
    Insert
    6His-SUMO-Nsp14 (ORF1ab SARS-CoV-2)
    Use
    Tags
    6His-Thrombin-SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    154956-plasmid-map-sequence-id-307845

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  32. 4LEC

    Plasmid
    #60091
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    METTL21A:JMC072-B07:C227766 (METTL21A Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  33. BPTF

    Plasmid
    #39111
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    BPTF (BPTF Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  34. pGEX6P1-DEST-Myc

    Plasmid
    #119752
    Purpose
    Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal Myc tag.
    Depositor
    Andrew Jackson, Martin Reijns
    Type
    Empty backbone
    Use
    Tags
    GST and Myc
    Expression
    Bacterial
    Mutation
    Promoter
    tac promoter
    Available since
    Feb. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    119752-plasmid-map-sequence-id-233674

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  35. HAO1

    Plasmid
    #38970
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    HAO1 (HAO1 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    38970-plasmid-map-sequence-id-471362

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  36. pFLAG_G495R∆2CARD

    Plasmid
    #216801
    Purpose
    Expresses a single point mutant of melanoma differentiation-associated protein 5 lacking its 2 CARD domains
    Depositor
    Sun Hur
    Insert
    melanoma differentiation-associated protein 5 delta 2 CARD domains (IFIH1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    G495R/∆1-287
    Promoter
    Available since
    May 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    216801-plasmid-map-sequence-id-433884

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  37. CLK3

    Plasmid
    #38831
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CLK3 (CLK3 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  38. Reduced Expression Alpha-5-Integrin-GFP

    Plasmid
    #30450
    Depositor
    Rick Horwitz
    Insert
    alpha 5 integrin (ITGA5 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    91-544 of enhancer region of CMV promoter removed…
    Promoter
    Available since
    Aug. 29, 2011
    Availability
    Academic Institutions and Nonprofits only

  39. KAT2B

    Plasmid
    #39002
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    KAT2B (KAT2B Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  40. BLVRA

    Plasmid
    #38856
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    BLVRA (Blvra Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 5601 - 5640 of 8357 results