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lentiCRISPRv2-TTF-1-gRNA

Plasmid

#101857

Purpose

Introducing a gRNA targeting human TTF-1 (NKX2-1) into cells

Depositor

David Mu

Publication

Lai et al Oncogene. 2018 Mar 19. pii: 10.1038/s413

Insert

gRNA targeting TTF-1 (NKX2-1 Human)

Use

Lentiviral

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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DAPK1_HUMAN_D0

Plasmid

#79728

Purpose

This plasmid encodes the kinase domain of DAPK1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Publication

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

DAPK1 (DAPK1 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
STK24_HUMAN_D0

Plasmid

#79742

Purpose

This plasmid encodes the kinase domain of STK24. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Publication

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

STK24 (STK24 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
KC1E_HUMAN_D0

Plasmid

#79724

Purpose

This plasmid encodes the kinase domain of KC1E. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Publication

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

KC1E (CSNK1E Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
MK13_HUMAN_D0

Plasmid

#79693

Purpose

This plasmid encodes the kinase domain of MK13. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Publication

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

MK13

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA4/TO CD16.7-h-PKD1 FLM R4114A

Plasmid

#83461

Purpose

Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and a mutation in the first Calmodulin binding domain (503)

Depositor

Thomas Weimbs

Publication

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Polycystin-1 (PKD1 Human)

Tags

CD16.7

Expression

Mammalian

Mutation

Amino-acids 4078-4303, R4113A

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-Zfp36-3'UTR

Plasmid

#61780

Purpose

Luciferase reporter containing the 3'UTR of mouse Zfp36

Depositor

Judy Lieberman

Publication

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Zfp36 3'UTR

Use

Luciferase

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pBacPak-HA-E(y)2

Plasmid

#52288

Purpose

Expresses Drosophila E(y)2

Depositor

Jerry Workman

Publication

Mohan et al Genes Dev. 2014 Feb 1;28(3):259-72. doi:

Insert

E(y)2 (e(y)2 Fly)

Tags

HA

Expression

Insect

Available since

April 24, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-GFP-IMP2-2_antisense-control

Plasmid

#42174

Depositor

Alexandra Kiemer

Publication

Kessler et al Am J Physiol Gastrointest Liver Physiol.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Insulin-like growth factor 2 mRNA binding protein 2-2 antisense control (IGF2BP2 Human)

Tags

GFP

Expression

Mammalian

Promoter

T7

Available since

June 14, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
HNF4A_P2-135

Plasmid

#31067

Depositor

Gerhart Ryffel

Publication

Thomas et al Hum Mol Genet. 2001 Sep 15. 10(19):2089-

Insert

HNF4A P2 promoter (HNF4A Human)

Use

Luciferase

Expression

Mammalian

Mutation

-135 to -1 of P2 HNF4A promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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Bov.hsc70.RSET.a1-554.wt(NarI)

Plasmid

#12533

Depositor

David McKay

Publication

Ha et al J Biol Chem. 1997 Oct 31. 272(44):27796-

Insert

Bovine 70 kDa heat shock cognate protein fragment amino acids 1-554

Expression

Bacterial

Mutation

deleted carboxy-terminal residues 555-650 of bovi…

Available since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only

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mCherry-ATG2C(VPS13C)

Plasmid

#232868

Purpose

Expresses the ATG2C domain of VPS13C in mammalian cells

Depositor

Pietro De Camilli

Publication

Wang et al Nat Cell Biol. 2025 May;27(5):776-789. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

the ATG2C domain of VPS13C (VPS13C Human)

Tags

mCherry

Expression

Mammalian

Available since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLVX-TetOne-Puro-MCL1

Plasmid

#202663

Purpose

Tet-ON 3rd generation expression vector with inducible expression of MCL1 and a puromycin selection cassette

Depositor

Jason Sheltzer

Publication

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

MCL1 apoptosis regulator, BCL2 family member (MCL1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-PSAT

Plasmid

#74115

Purpose

Expression of PSAT

Depositor

Lewis Cantley

Publication

Mullarky et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

Insert

pET28a-PSAT (PSAT1 Human)

Expression

Bacterial and Mammalian

Available since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pGCaMP5-DHFR

Plasmid

#78706

Purpose

GCaMP5 under the Toxoplasma SAG1 5' UTR. Encodes a DHFR selectable marker.

Depositor

Sebastian Lourido

Publication

Sidik et al J Biol Chem. 2016 Apr 29;291(18):9566-80

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

GCaMP5
DHFR

Promoter

Toxoplasma SAG1 5' UTR

Available since

May 27, 2016

Availability

Academic Institutions and Nonprofits only

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pRF+275Dux4

Plasmid

#21293

Depositor

Stephen Tapscott

Publication

Snider et al Hum Mol Genet. 2009 Jul 1;18(13):2414-30

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

DUX 4 (DUX4 Human, DUX 4)

Use

Luciferase

Available since

July 22, 2009

Availability

Academic Institutions and Nonprofits only
pLD-puro-Cc-TARDBP-WT_VA

Plasmid

#141327

Purpose

Lentiviral vector for expression of C-terminally VA-tagged human TARDBP-WT in mammalian cells

Depositor

Mohan Babu

Publication

Pourhaghighi et al Cell Syst. 2020 Apr 22;10(4):333-350.e14

Insert

TARDBP-WT (TARDBP Human)

Use

Lentiviral

Tags

VA tag (Beacon-2XStrep-6XHis-2XTEV-3XFLAG)

Expression

Mammalian

Available since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223_FBXW7_WT

Plasmid

#81973

Purpose

Gateway Donor vector containing FBXW7, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

FBXW7 (FBXW7 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_IDH2_p.R172K

Plasmid

#81432

Purpose

Gateway Donor vector containing IDH2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IDH2 (IDH2 Human)

Use

Gateway Cloning

Mutation

R172K

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SMAD5_WT

Plasmid

#82217

Purpose

Gateway Donor vector containing SMAD5 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SMAD5 (SMAD5 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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