We narrowed to 18,218 results for: Met;

1. Str-KDEL_SBP-EGFP-Ecadherin

   Plasmid

   #65286

   Purpose

   synchronize trafficking of Ecadherin from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Ecadherin
   

   Use

   Tags

   EGFP and IL-2 Signal Sequence and Streptavidin Bi…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. CL7-LbCas12a

   Plasmid

   #164658

   Purpose

   Bacterial expression of LbCas12a using CL7/Im7

   Depositor

   Piyush Jain

   Article

   Nguyen et al Methods. 2021 Feb 9. pii: S1046-2023(21)

   Insert

   LbCas12a
   

   Use

   Tags

   Thioredoxin, CL7, SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCry2PHR-mCh-LRP6c

   Plasmid

   #42960

   Purpose

   Expresses optogenetically controlled LRP6 (C-terminal domain)

   Depositor

   David Schaffer

   Article

   Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

   Insert

   Cry2PHR-mCherry-LRP6c
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   LPR6 cytoplasmic domain only

   Promoter

   CMV

   Available since

   April 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET251-pUC 12xMS2V6 Loxp KANr Loxp

   Plasmid

   #104392

   Purpose

   Yeast endogenous mRNA tagging

   Depositor

   Evelina Tutucci, Robert Singer

   Article

   Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

   Insert

   12xMS2V6
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   All Loops are U-variants, interspaced by 50 nucle…

   Promoter

   Available since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. modRNAc0-p53DD

   Plasmid

   #176902

   Purpose

   IVT template plasmid for making p53DD modRNA

   Depositor

   Xiaojun Lian

   Article

   Haideri et al Cell Rep Methods. 2022 Sep 7;2(9):100290

   Insert

   p53DD
   

   Use

   Ivt template plasmid

   Tags

   Expression

   Mutation

   Promoter

   T7 promoter

   Available since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Str-KDEL_SBP-mCherry-Ecadherin

   Plasmid

   #65287

   Purpose

   synchronize trafficking of Ecadherin from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Ecadherin
   

   Use

   Tags

   IL-2 Signal Sequence and Streptavidin Binding Pro…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. Clover-Geminin(1-110)

   Plasmid

   #83915

   Purpose

   Fluorescent probe for M/G1 transition

   Depositor

   Michael Lin

   Article

   Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   Clover-Geminin(1-110)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. GI-Gal4 DBD

   Plasmid

   #42500

   Purpose

   GIGANTEA fused with Gal4 DNA binding domain (DBD), part of a light-inducible transcription factor

   Depositor

   Ricardo Dolmetsch

   Article

   Yazawa et al Nat Biotechnol. 2009 Oct;27(10):941-5. d

   Insert

   GIGANTEA fused with Gal4 DBD
   

   Use

   Tags

   Gal4 DNA binding domain (DBD)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEB2-mKate2

   Plasmid

   #104009

   Purpose

   Plasmid encoding mKate2

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   mKate2
   

   Use

   Low copy

   Tags

   Expression

   Bacterial

   Mutation

   Mutations relative to eqFP578 (R32G, K42R, V45A, …

   Promoter

   proC

   Available since

   Dec. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. mRuby2-Rab5a-7

    Plasmid

    #55911

    Purpose

    Localization: Ras GTPase, Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    Rab5a
    

    Use

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pCytERM_mScarlet-i_N1

    Plasmid

    #85068

    Purpose

    In vivo visualization of the ER (can be used for colocalization studies and the OSER assay)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    CytERM
    

    Use

    Tags

    mScarlet-i

    Expression

    Mammalian

    Mutation

    aa1-29

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEDA5_GFPmut3_Y66H

    Plasmid

    #80085

    Purpose

    Positive control plasmid for Nicking Mutagenesis. Constitutively expresses a non-fluorescent variant of GFPmut3 (Y66H) that is mutated to fluorescent (H66Y) in the protocol.

    Depositor

    Timothy Whitehead

    Article

    Wrenbeck et al Nat Methods. 2016 Nov;13(11):928-930. do

    Insert

    GFPmut3_Y66H
    

    Use

    Tags

    6x-His tag

    Expression

    Bacterial

    Mutation

    changed Tyrosine 66 to Histidine

    Promoter

    proB

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTRIPZ-Wt-PTEN

    Plasmid

    #206982

    Purpose

    Lentiviral vector for expressing doxycyclin-inducible cDNA of wild type PTEN

    Depositor

    Ming Chen

    Article

    Loh et al Metabolites. 2023 Aug 11;13(8):939. doi:

    Insert

    PTEN (PTEN Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tet-on promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. Ii-Str_ManII-SBP-tagBFP

    Plasmid

    #65257

    Purpose

    synchronize trafficking of ManII from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Ii-Streptavidin and truncated Mannosidase II
    

    Use

    Tags

    Streptavidin Binding Protein (SBP) and tagBFP

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 116 of ManII

    Promoter

    CMV

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA-mCherry-4A/4B-CAAX

    Plasmid

    #87699

    Purpose

    Express mCherry with a peptide linker (contaning a HCVp 4A/4B substrate site) and a C-terminal CAAX box from KRras for plasma-membrane targeting in mammalian cells

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    mCherry-4A/4B-CAAX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMK345 (Hygro-P2A-mAID-mClover)

    Plasmid

    #121179

    Purpose

    N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    Hygro-P2A-mAID-mClover
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTriEx-mCherry-LOV2 V416L

    Plasmid

    #81036

    Purpose

    mammalian expression of LOV V416L, a mutant with super long relaxation time

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    LOV V416L
    

    Use

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    V416L

    Promoter

    Available since

    Aug. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. hCas9-VPR

    Plasmid

    #68497

    Purpose

    nuclease competent SP-Cas9 fused to VPR

    Depositor

    George Church

    Article

    Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

    Insert

    SP-Cas9-VPR
    

    Use

    Tags

    VPR

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. PB-hEF1A-MG-U6-sgRNA

    Plasmid

    #175505

    Purpose

    All-in-one piggyBac transposon destination vector for hEF1a expression of Mega Gate cloned elements and hU6 expression of Golden Gate cloned guide RNAs

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hEF1a, U6

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDH_Str-KDEL_neomycin

    Plasmid

    #65307

    Purpose

    Luminal ER hook only (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Streptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end
    

    Use

    Lentiviral

    Tags

    KDEL motif and Signal Sequence

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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