We narrowed to 28,253 results for: Tat

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bdSUMO-pHD118-GFP-HiBit

Plasmid

#171015

Purpose

For bacterial expression of bdSUMO tagged muGFP with pHD118 peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-pHD118-muGFP-HiBiT

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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bdSUMO-pHlip-GFP-HiBit

Plasmid

#171016

Purpose

For bacterial expression of bdSUMO tagged muGFP with pHlip peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-pHlip-muGFP-HiBiT

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCold TDP43 M337V

Plasmid

#27453

Depositor

Aaron Gitler

Article

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Tags

Factor Xa and His6

Expression

Bacterial

Mutation

Methionine 337 mutated to Valine (M337V)

Available Since

Feb. 10, 2011

Availability

Academic Institutions and Nonprofits only
pRS416 Gal Q331K YFP

Plasmid

#27450

Depositor

Aaron Gitler

Article

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Tags

YFP

Expression

Yeast

Mutation

Glutamine 331 mutated to Lysine (Q331K)

Available Since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only
pGL3 basic Mut E2

Plasmid

#48748

Purpose

Expression of luciferase driven by mPer2 promoter fragment containing mutated E-box2 (bases -112 to +98 with respect to transcription start site at +1)

Depositor

Joseph Takahashi

Article

Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10

Insert

mPer2 promoter/enhancer region containing mutated E-box2 (-112 to +98)

Use

Luciferase

Mutation

Mutated E-box2 sequence from CACGTT to GCTAGT

Available Since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pDuet TDP-43 G294A

Plasmid

#27463

Depositor

Aaron Gitler

Article

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Use

Coexpression

Expression

Bacterial

Mutation

Glycine 294 mutated to Alanine (G294A)

Available Since

Feb. 10, 2011

Availability

Academic Institutions and Nonprofits only
pQE32-pr-mEosFP (mEosFP-V69T)

Plasmid

#99213

Purpose

Bacterial expression of the primed conversion capable protein pr-mEosFP (mEosFP-V69T).

Depositor

Periklis Pantazis

Article

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-mEosFP

Expression

Bacterial

Mutation

monomeric variant with mutation V69T for primed c…

Promoter

T5

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-shRNA_Tet3

Plasmid

#85740

Purpose

shRNA against mouse Tet3

Depositor

Hongjun Song

Article

Yu et al Nat Neurosci. 2015 Jun;18(6):836-43. doi

Insert

Tet3 (Tet3 Mouse)

Use

AAV

Tags

EYFP

Expression

Mammalian

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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Affibody HER2:243.BirA*-pET301/NT-DEST

Plasmid

#174692

Purpose

Plasmid encoding for the bacterial expression of a bispecific coding for the anti-HER2 affibody ZHER2:342 fused to the mutated form of BirA enzyme, BirA*

Depositor

Jean Gariepy

Article

Alwash et al ACS Pharmacol Transl Sci. 2022 Feb 1;5(2

Insert

Anti-HER2 affibody ZHER2:342 fused to mutated form of BirA, BirA*

Tags

HIS tag

Expression

Bacterial

Mutation

R118G mutation in BirA

Promoter

T7 promoter

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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POLArISact

Plasmid

#164971

Purpose

Expresses POLArISact, an orientation probe that specifically binds to F-actin, in mammalian cells

Depositor

Sumio Terada

Article

Sugizaki et al Proc Natl Acad Sci U S A. 2021 Mar 16;11

Insert

POLArISact

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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FKBP-CD28TMD-NTEVp(H75E)

Plasmid

#137829

Purpose

MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal H75E mutant TEV protease

Depositor

Joshua Leonard

Article

Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

Insert

FKBP-CD28TMD-NTEVp(H75E)

Tags

3x FLAG

Expression

Mammalian

Mutation

Mutated Histidine 75 to Glutamic Acid in NTEVp fr…

Promoter

CMV

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pBAD-eLACCO2.1

Plasmid

#208017

Purpose

Biosensor for extracellular L-lactate

Depositor

Robert Campbell

Article

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

eLACCO2.1

Expression

Bacterial

Available Since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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mVenus-IQ6 R6A

Plasmid

#198202

Purpose

FRET acceptor: Mutated IQ6 domain of Moysin Va tagged with N-terminal mVenus (lower binding affinity)

Depositor

Christian Wahl-Schott

Article

Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

Insert

MYO5A IQ domain (Myo5a Mouse)

Tags

mVenus

Expression

Mammalian

Mutation

R6A

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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PatoL-OG241

Plasmid

#72841

Purpose

Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Bba_B0034 (iGEM). OG241 backbone.

Depositor

Chris Barnes

Article

Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

Insert

PatoL

Expression

Bacterial

Promoter

PatoL

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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myc-hRAD18

Plasmid

#68827

Purpose

Expresses c-myc-tagged human RAD18

Depositor

Satoshi Tateishi

Article

Watanabe et al Nucleic Acids Res. 2009 Apr;37(7):2176-9

Insert

c-myc tagged human RAD18 (RAD18 Human)

Tags

c-myc

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only
pAM4941 (pCV0053)

Plasmid

#120083

Purpose

Cyanobacteria cloning vector. Chromosomal integration into Synechococcus elongatus neutral site 3 (NS3).

Depositor

Susan Golden

Article

Taton et al Nucleic Acids Res. 2014 Jul 29. pii: gku

Insert

Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-S7942NS3-RK2BOM, CV-aacC1, CV-ccdB-SwaI

Use

Other

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pBluescript GPX-1

Plasmid

#13038

Depositor

Peter Newburger

Article

Shen et al J Biol Chem. 1993 May 25. 268(15):11463-

Insert

GPX-1 (GPX1 Human)

Expression

Bacterial

Mutation

Additional GCG upstream of codon 11. Codon 92 is …

Available Since

Nov. 7, 2006

Availability

Academic Institutions and Nonprofits only
Man2a1-myc

Plasmid

#163648

Purpose

Expresses myc tagged Man2a1 in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

mannosidase alpha class 2A member 1 (MAN2A1 Human)

Tags

myc

Expression

Mammalian

Mutation

Silent mutation was made to introduce a XbaI rest…

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pRS416 Gal M337V YFP

Plasmid

#27449

Depositor

Aaron Gitler

Article

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Tags

YFP

Expression

Yeast

Mutation

Methionine 337 mutated to Valine (M337V)

Available Since

Feb. 10, 2011

Availability

Academic Institutions and Nonprofits only
NLS-CanlonicSF

Plasmid

#188390

Purpose

To explore lactate dynamics in intact systems

Depositor

Alejandro San Martín

Article

Aburto et al ACS Sens. 2022 Oct 28. doi: 10.1021/acss

Insert

NLS-CanlonicSF

Tags

NLS and myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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