We narrowed to 142,857 results for: ins

1. pUC19-U6-MbCas12a crRNA-BsmBI cassette (BPK4449)

   Plasmid

   #114088

   Purpose

   U6 promoter crRNA entry vector used for all MbCas12a crRNAs (clone spacer oligos into BsmBI cassette)

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   entry vector for MbCas12a crRNAs
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV 3xFLAG-ATG3 K243R

   Plasmid

   #129301

   Purpose

   Expresses pCMV 3xFLAG-ATG3 K243R in mammalian cells

   Depositor

   Robin Ketteler

   Article

   Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

   Insert

   Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   changed Lysine 243 to Arginine

   Promoter

   CMV

   Available since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTWIN1-His6-Ssp-Httex1-29Q

   Plasmid

   #84350

   Purpose

   Expression of the human Huntingtin Exon1 protein containing 29Q in E.coli

   Depositor

   Hilal Lashuel

   Article

   Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

   Insert

   Huntingtin Exon 1 (HTT Human)
   

   Use

   Tags

   N-terminal Ssp intein (His-tagged)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTWIN1-His6-Ssp-Httex1-37Q

   Plasmid

   #84351

   Purpose

   Expression of the human Huntingtin Exon1 protein containing 37Q in E.coli

   Depositor

   Hilal Lashuel

   Article

   Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

   Insert

   Huntingtin Exon 1 (HTT Human)
   

   Use

   Tags

   N-terminal Ssp intein (His-tagged)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTWIN1-His6-Ssp-Httex1-15Q

   Plasmid

   #84348

   Purpose

   Expression of the human Huntingtin Exon1 protein containing 15Q in E.coli

   Depositor

   Hilal Lashuel

   Article

   Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

   Insert

   Huntingtin Exon1 (HTT Human)
   

   Use

   Tags

   N-terminal Ssp intein (His-tagged)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFB-GST-FBXO22

   Plasmid

   #228367

   Purpose

   express human FBXO22 in insect cells, such as Trichoplusia ni Hi5

   Depositor

   Ning Zheng

   Article

   Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

   Insert

   FBXO22 (FBXO22 Human)
   

   Use

   Tags

   GST

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFB-His-SKP1

   Plasmid

   #228368

   Purpose

   express human SKP1 in insect cells, such as Trichoplusia ni Hi5

   Depositor

   Ning Zheng

   Article

   Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

   Insert

   SKP1 (SKP1 Human)
   

   Use

   Tags

   His

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pACE-MBP-FBXL17 (310–701)

   Plasmid

   #228369

   Purpose

   express human FBXL17 in insect cells, such as Trichoplusia ni Hi5

   Depositor

   Ning Zheng

   Article

   Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

   Insert

   FBXL17 (FBXL17 Human)
   

   Use

   Tags

   MBP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. Strep-His-Tev-hCGNL1

   Plasmid

   #236520

   Purpose

   For expression of Paracingulin (human) in insect cells

   Depositor

   Sandra Citi

   Article

   Rouaud et al J Cell Biol. 2023 Jul 3;222(7):e20220806

   Insert

   CGNL1 (CGNL1 Human)
   

   Use

   Tags

   2xStrep-10xHis-Tev

   Expression

   Insect

   Mutation

   WT

   Promoter

   Available since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAED2

    Plasmid

    #234599

    Purpose

    Fluorescent reporter of the SOS response

    Depositor

    Tanel Tenson

    Article

    Kaldalu et al NPJ Antimicrob Resist. 2025 Apr 11;3(1):

    Inserts

    mScarlet-I
    gfp-mut2
    

    Use

    Reporter

    Tags

    Expression

    Bacterial

    Mutation

    GFPmut2 was derived from avGFP with the following…

    Promoter

    Ptet+dnaK P1 and cda

    Available since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCR4_miniFXN_8

    Plasmid

    #234361

    Purpose

    Mini FXN plasmid with a partial deletion of the 5' UTR and a large deletion of intron 1 in FXN. Does not contain GAA repeats.

    Depositor

    Marek Napierala

    Article

    Li et al Hum Gene Ther. 2020 Aug;31(15-16):839-85

    Insert

    FXN (FXN Human)
    

    Use

    Cloning

    Tags

    Flag Tag

    Expression

    Mutation

    There is a partial deletion in the 5'UTR of …

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. lenti_U6_miR-Oct4e_TRE12_OCT4(co)-mNeonGreen_WPRE

    Plasmid

    #229544

    Purpose

    Lentiviral construct to express synthetic miRNA and OCT4-mNeonGreen fusion protein

    Depositor

    Domitilla Del Vecchio

    Article

    Ilia et al Sci Adv. 2023 Dec;9(48):eadg8495. doi: 1

    Insert

    Oct4(co)-mNeonGreen (POU5F1 Human)
    

    Use

    Lentiviral

    Tags

    Oct4-mNeonGreen

    Expression

    Mammalian

    Mutation

    codon-optimized OCT4

    Promoter

    TRE12

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRetroX-TRE3G-GMIP P363D P364D P366D

    Plasmid

    #221323

    Purpose

    Dox-inducible expression of GMIP mutant in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    GMIP (Gmip Mouse)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mutation

    P363D P364D P366D

    Promoter

    TRE3G

    Available since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRetroX-TRE3G-IRSp53-T2A-Rac1(G12V)

    Plasmid

    #221330

    Purpose

    Dox-inducible expression of FLAG-IRSp53-T2A-HA-Rac1 G12V in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    FLAG-IRSp53 and HA-Rac1(G12V) (Baiap2, Rac1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    G12V

    Promoter

    TRE3G

    Available since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMSCV-FKBP-MAVS(pLxIS) E433A

    Plasmid

    #221258

    Purpose

    Expression of MAVS pLxIS (393-441) mutant in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    MAVS pLxIS (393-441) (Mavs Mouse)
    

    Use

    Retroviral

    Tags

    FLAG-4xFKBP

    Expression

    Mutation

    E433A

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMSCV-MAVS(GMIP pLxIS)

    Plasmid

    #221316

    Purpose

    Expression of MAVS chimera with GMIP pLxIS domain in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    MAVS chimera with GMIP pLxIS domain (Mavs Mouse)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMSCV-MAVS(pLxIS trunc)

    Plasmid

    #221314

    Purpose

    Expression of MAVS truncated pLxIS domain in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    MAVS truncated pLxIS domain (Mavs Mouse)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMSCV-FKBP-MAVS(trunc Cterm)

    Plasmid

    #221259

    Purpose

    Expression of MAVS truncation (393-431) in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    MAVS truncation (393-431) (Mavs Mouse)
    

    Use

    Retroviral

    Tags

    3xFLAG-4xFKBP

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMSCV-FKBP-STING(pLxIS) S365A

    Plasmid

    #221241

    Purpose

    Expression of mutant STING CTT in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    STING CTT (Sting1 Mouse)
    

    Use

    Retroviral

    Tags

    3xFLAG-4xFKBP

    Expression

    Mutation

    S356A

    Promoter

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMSCV-FKBP-STING(pLxIS) L373A

    Plasmid

    #221242

    Purpose

    Expression of mutant STING CTT in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    STING CTT (Sting1 Mouse)
    

    Use

    Retroviral

    Tags

    3xFLAG-4xFKBP

    Expression

    Mutation

    L373A

    Promoter

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge