We narrowed to 3,095 results for: sam

1. pSL3402 (pTnsABf_Pse)

   Plasmid

   #200901

   Purpose

   Expresses human codon-optimized Pseudoalteromonas TnsA-TnsB fusion from a CMV promoter.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   PseTnsA-TnsB
   

   Tags

   Internal NLS between TnsA-TnsB fusion

   Expression

   Mammalian

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSL1749 (pDonor, R*MmeI)

   Plasmid

   #160733

   Purpose

   Mini-transposon donor plasmid for V. cholerae CAST system. Mini-transposon has MmeI site in R end for Tn-seq. pUC19 vector backbone.

   Depositor

   Samuel H. Sternberg

   Article

   Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

   Insert

   VchCAST donor DNA
   

   Expression

   Bacterial

   Promoter

   N/A

   Available Since

   Oct. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-28c(+)-YTHDF3

   Plasmid

   #102276

   Purpose

   For bacterial expression of His-tagged human YTHDF3

   Depositor

   Samie Jaffrey

   Article

   Patil et al Nature. 2016 Sep 7;537(7620):369-373. do

   Insert

   YTHDF3 (YTHDF3 Human)
   

   Tags

   6X HIS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSL0527 (pDonor)

   Plasmid

   #130634

   Purpose

   Encodes a mini-transposon derived from V. cholerae CAST system, with CmR cargo gene. Total transposon size = 977 bp.

   Depositor

   Samuel H. Sternberg

   Article

   Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

   Insert

   VchCAST donor DNA (Mini-Tn)
   

   Use

   CRISPR; Transposon

   Expression

   Bacterial

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNIFV

   Plasmid

   #111085

   Purpose

   pNIFV is a vector plasmid with mutations in the cis-acting region, to prevent correction of the integrase by recombination when used with pCiPS-NIFV to produce Non-Integrating Foamy Virus vectors

   Depositor

   David Russell

   Article

   Deyle et al J Virol. 2010 Sep;84(18):9341-9. doi: 10

   Insert

   empty backbone/deleted foamy vector, same as pΔΦ with mutations in cis-acting pol region
   

   Use

   Deleted foamy virus

   Mutation

   at nt 2334 A changed to T, at nt 2495 A changed t…

   Promoter

   CMV

   Available Since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. Perturb-FISH

   Plasmid

   #220626

   Purpose

   expression of gRNAs under U6T7 promoter

   Depositor

   Samouil Farhi

   Article

   Binan et al Cell. 2025 Mar 10:S0092-8674(25)00197-7.

   Insert

   sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   BsmBI

   Available Since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. BS-TRP1-TetOff(7repeats)-GFP-Pho8

   Plasmid

   #207011

   Purpose

   Tet-Off controlled GFP-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   Pho8
   

   Tags

   GFP

   Expression

   Yeast

   Available Since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. SUPV3L1-HA WT

   Plasmid

   #232348

   Purpose

   Expresses human SUPV3L1/SUV3 in mammalian cells with HA tag

   Depositor

   Samantha Lewis

   Article

   Begeman et al bioRxiv [Preprint]. 2024 Aug 17:2024.08.

   Insert

   SUPV3L1 (SUPV3L1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMMB67EH-Spy

   Plasmid

   #90102

   Purpose

   IPTG-inducible CFP/YFP FRET-based biosensor for cyclic-di-GMP measurement using YcgR binding site.

   Depositor

   Samuel Miller

   Article

   Christen et al Science. 2010 Jun 4;328(5983):1295-7. do

   Insert

   YcgR (ycgR )
   

   Use

   Broad-host range

   Expression

   Bacterial

   Promoter

   Ptac

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSL0341 (pTarget)

    Plasmid

    #200906

    Purpose

    Encodes an mCherry casette under a minimal CMV promoter. Used as the target plasmid for plasmid-based integration assays, and for plasmid-based transcriptional activation.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    pTarget
    

    Expression

    Mammalian

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pChlamy/Cre

    Plasmid

    #205440

    Purpose

    An expression vector for Cre recombinase (codon usage for C. reinhardtii)

    Depositor

    Masamichi Kamihira

    Article

    Huang et al J Biosci Bioeng. 2021 Nov;132(5):469-478

    Insert

    Cre recombinase gene
    

    Use

    Chlamydomonas expression

    Promoter

    Heat shock protein 70A (HSP70A)/ribulose bisphosp…

    Available Since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. BS-TRP1-TetOff(7 repeats)-mRuby3-Pho8

    Plasmid

    #207031

    Purpose

    Tet-Off controlled mRuby3-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    Pho8
    

    Tags

    mRuby3

    Expression

    Yeast

    Available Since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSL2783 (pTnsC-VP64_Vch)

    Plasmid

    #200896

    Purpose

    Expresses human codon-optimized V. cholerae TnsC-VP64 from a CMV promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    VchTnsC-VP64
    

    Tags

    NLS

    Expression

    Mammalian

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. Incoherent feedforward (IFF) plasmid

    Plasmid

    #169748

    Purpose

    Standalone IFF circuit plasmid that has the same implementation of the IFF subcircuit in Equalizer-L.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    miR(FF4) target-eGFP-miR(FF4) target-miR(FF4)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV-tetO2

    Available Since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pConRef-2H12

    Plasmid

    #221182

    Purpose

    cdGreen expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11213)

    Depositor

    Urs Jenal

    Article

    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

    Inserts

    cdGreen
    mScarlet-I
    

    Expression

    Bacterial

    Mutation

    N-terminus changed: starts with MSKKYGEAVIKE ...

    Promoter

    Constitutive and None (same as cdGreen)

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pU6T7

    Plasmid

    #71462

    Purpose

    Expresses gRNA from hybrid human U6/T7 promoter for both cellular expression and in vitro transcription from same promoter (2 Gs at initiation)

    Depositor

    Willie Mark

    Article

    Romanienko et al PLoS One. 2016 Feb 5;11(2):e0148362. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial and Mammalian

    Promoter

    human U6/phage T7

    Available Since

    Feb. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSL2669 (pTnsABf_Vch)

    Plasmid

    #200897

    Purpose

    Expresses human codon-optimized V. cholerae TnsA-TnsB fusion from a CMV promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    VchTnsA-TnsB
    

    Tags

    Internal NLS between TnsA-TnsB fusion

    Expression

    Mammalian

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSL0915 (CRISPR with target 3)

    Plasmid

    #130643

    Purpose

    Expresses CRISPR RNA from a T7 promoter for VchCAST system. The CRISPR array encodes a guide RNA that targets lacZ.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    CRISPR(target-3)
    

    Use

    CRISPR; Transposon

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28C-mCherry-YTHDF2

    Plasmid

    #204406

    Purpose

    E. coli expression of full-length YTHDF2 with an N-terminal mCherry tag

    Depositor

    Samie Jaffrey

    Article

    Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

    Insert

    YTHDF2 (YTHDF2 Human)
    

    Tags

    6x-His; mCherry

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pU6T7G

    Plasmid

    #71463

    Purpose

    Expresses gRNA from hybrid human U6/T7 promoter for both cellular expression and in vitro transcription from same promoter (1 G at initiation)

    Depositor

    Willie Mark

    Article

    Romanienko et al PLoS One. 2016 Feb 5;11(2):e0148362. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial and Mammalian

    Promoter

    human U6/phage T7

    Available Since

    Feb. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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