We narrowed to 7,125 results for: trac

1. AAV-CAG-FLEX-HA-DLX2-T2A-mCherry

   Plasmid

   #178585

   Purpose

   Cre-dependent expression of HA-DLX2 and mCherry under the constitutive promoter

   Depositor

   Chun-Li Zhang

   Article

   Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

   Insert

   DLX2 (DLX2 Human)
   

   Use

   AAV

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKT2/CAGXSP/PalmNanoLuc

   Plasmid

   #182969

   Purpose

   Stably express PalmNanoLuc in mammalian cells via the sleeping beauty transposon system.

   Depositor

   Masamitsu Kanada

   Article

   Perez et al Adv Genet (Hoboken). 2022 Jan 19;3(1):21

   Insert

   PalmNanoLuc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Available since

   April 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. hsDCX-EGFP-pHAT

   Plasmid

   #83918

   Purpose

   human Doublecortin, N-terminal 6-His tag, C-terminal EGFP-Strep-tag II

   Depositor

   Gary Brouhard

   Article

   Bechstedt et al Dev Cell. 2012 Jul 17;23(1):181-92. doi:

   Insert

   Doublecortin isoform b (DCX Human, NM_178152.2)
   

   Use

   Tags

   6-His and EGFP+Strep-tag II

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGLTR-S-PURO

   Plasmid

   #58244

   Purpose

   GATEWAY-compatible lentiviral RNAi delivery vector; SFFV-driven Puromycin resistance

   Depositor

   Stephan Geley

   Article

   Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi:

   Inserts

   attR1-Cm(R)-CcdB-attR2
   Puro(R)
   

   Use

   Lentiviral and RNAi; Gateway destination vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV and no

   Available since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. 3611 pcDNA3 Bim

   Plasmid

   #8804

   Depositor

   Stanley Korsmeyer

   Article

   Harada et al Proc Natl Acad Sci U S A 2004 Oct 26;101

   Insert

   Bim (Bcl2l11 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 23, 2006

   Availability

   Academic Institutions and Nonprofits only

6. eGFP-KCC2

   Plasmid

   #104075

   Purpose

   expresses rat KCC2 (b isoform) with eGFP tag at the N-terminus of KCC2

   Depositor

   Igor Medyna

   Article

   Pellegrino et al J Physiol. 2011 May 15;589(Pt 10):2475-9

   Insert

   KCC2 (b isoform) (Slc12a5 Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   EcoR1 site in rat KCC2 was removed by silent muta…

   Promoter

   CMV

   Available since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. mEos3-Rac1

   Plasmid

   #92039

   Purpose

   Expresses mEos3-Rac1 fusion protein

   Depositor

   Ji Yu

   Article

   Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

   Insert

   RAC1 (RAC1 Human)
   

   Use

   Tags

   mEos3

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   CMV

   Available since

   June 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. Sema3a(L, del13)-AP-His

   Plasmid

   #72011

   Purpose

   Expresses the Sema3A protein (truncated at cleavage site P3; ie, long and missing exon 13), C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Sema3a (Sema3a Mouse)
   

   Use

   Tags

   AP-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pB80-iLID-mCherry-RAB11

   Plasmid

   #174623

   Purpose

   Optogenetic coupling to recycling endosomes (RAB11) via iLID

   Depositor

   Lukas Kapitein

   Article

   Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   iLID-mCherry-RAB11 (RAB11A Human, Synthetic)
   

   Use

   Tags

   iLID-mCherry

   Expression

   Mammalian

   Mutation

   mCherry: Met1Del; RAB11: Met1Del

   Promoter

   Chicken beta-actin

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. mEos3-dnRac1

    Plasmid

    #92040

    Purpose

    Expresses mEos3 fusion with dnRac1 (T17N).

    Depositor

    Ji Yu

    Article

    Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

    Insert

    RAC1 (RAC1 Human)
    

    Use

    Tags

    mEos3

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    T17N (dominant negative)

    Promoter

    CMV

    Available since

    June 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. GFP-Braf R188L

    Plasmid

    #118330

    Purpose

    Expression of human Braf

    Depositor

    Philip Stork

    Article

    Li et al J Biol Chem. 2013 Sep 20;288(38):27646-5

    Insert

    Braf R188L (BRAF Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    R188L

    Promoter

    CMV

    Available since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSpCas9_BB_2A-GFP_MAPRE1-gRNA#2

    Plasmid

    #107727

    Purpose

    Knock out of EB1 in human cells by CRISPR/Cas9

    Depositor

    Torsten Wittmann

    Article

    van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

    Insert

    MAPRE1 gRNA #1 (targets Exon 1) (MAPRE1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. mEos3-caRac1

    Plasmid

    #92041

    Purpose

    Expresses mEos3 fusion with caRac1 (Q61L)

    Depositor

    Ji Yu

    Article

    Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

    Insert

    RAC1 (RAC1 Human)
    

    Use

    Tags

    mEos3

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Q61L (constitutively active)

    Promoter

    CMV

    Available since

    June 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. MO91-ORAI1N223A-TEV-pHluorin (ORAI1-EC-pHluorin)

    Plasmid

    #174336

    Purpose

    The ORAI1N223A mutant containing-TEV-pHluorin-TEV sequence in the extracellular loop 2

    Depositor

    Sonal Srikanth

    Article

    Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

    Insert

    human Orai1 (ORAI1 Human)
    

    Use

    Retroviral

    Tags

    pHluorin inserted in the extracellular loop 2 of …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. dmBACCS2VL-IRES-dOrai-IRES-mCherry

    Plasmid

    #72898

    Purpose

    Mammalian expression of dmBACCS2VL, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and mCherry

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    dmBACCS2VL-IRES-dOrai-IRES-mCherry
    

    Use

    Tags

    IRES-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. mVenus-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax

    Plasmid

    #173866

    Purpose

    Mammalian expression of mVenus-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax (opto-Cdc42)

    Depositor

    Takao Nakata

    Article

    Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

    Insert

    mVenus-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax
    

    Use

    Tags

    C-terminal CAAX-box and mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX-4T-1 3xHA-MKK7a1-EE

    Plasmid

    #47578

    Purpose

    Bacterial expression plasmid for GST-3xHA-tagged constitutively active MKK7a1

    Depositor

    Kevin Janes

    Article

    Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

    Insert

    MKK7a1-EE (Map2k7 Mouse)
    

    Use

    Tags

    3xHA and GST

    Expression

    Bacterial

    Mutation

    Residues 73-420 of RefSeq sequence. Ser271 to Gl…

    Promoter

    Available since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

18. dmBACCS2NS-IRES-dOrai-IRES-mCherry

    Plasmid

    #72896

    Purpose

    Mammalian expression of dmBACCS2NS, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and mCherry

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    dmBACCS2NS-IRES-dOrai-IRES-mCherry
    

    Use

    Tags

    IRES-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. MO70 - Orai1 EC-Flag

    Plasmid

    #79590

    Purpose

    Transient expression and retroviral Expression

    Depositor

    Yousang Gwack

    Article

    Srikanth et al J Biol Chem. 2010 Feb 12;285(7):5066-75.

    Insert

    Orai1 (ORAI1 Human)
    

    Use

    Retroviral

    Tags

    FLAG (inserted between TM3 and TM4 of the protein…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pTBL1401 pLenti-pEF1a(short)-mNeonGreen-P2A-Cas9

    Plasmid

    #163644

    Purpose

    Makes a lentivirus that expresses mNeonGreen and Cas9.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Insert

    mNeonGreen-P2A-Cas9
    

    Use

    Lentiviral

    Tags

    mNeonGreen-P2A

    Expression

    Mutation

    Promoter

    EFS

    Available since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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