12,254 results

1. pASR102

   Plasmid

   #122636

   Purpose

   BglFusion compatible IPTG inducible integration vector for Streptococcus pneumoniae (pPEPZ Backbone)

   Depositor

   Jan-Willem Veening

   Article

   Keller et al Genes (Basel). 2019 May 22;10(5). pii: g

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Aug. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAVS1-Puro CAG-CLTX CD32a-tm CD3z CAR

   Plasmid

   #171963

   Purpose

   Donor plasmid for CLTX T-CAR expression in human cells

   Depositor

   Xiaoping Bao

   Article

   Chang et al Nat Commun. 2023 Apr 20;14(1):2266. doi:

   Insert

   Synthetic CLTX CD32a-tm CD3z CAR
   

   Use

   CRISPR, Synthetic Biology, and TALEN

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. LSB-hsa-miR-195-3p

   Plasmid

   #103311

   Purpose

   Used to sense miRNA activity using fluorescence based tools. hsa-miR-195-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

   Depositor

   Ron Weiss

   Article

   Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

   Insert

   hsa-miR-195-3p target (MIR195 Human)
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBEST-p70a-UTR1-NTerm 6xHis-zmADH-T500

   Plasmid

   #92228

   Purpose

   Expression of zmADH using the p70a promoter and UTR1

   Depositor

   Lital Alfonta

   Article

   Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

   Insert

   NTerm 6xHis Tagged zmADH
   

   Use

   Synthetic Biology; Cell free protein synthesis (c…

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   p70a

   Available Since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. CMV-10xPP7 oligo-library based

   Plasmid

   #158199

   Purpose

   Cassette of 10 different binding sites with high affinity to PP7 phage coat protein, as extracted from the oligo pool experiment.

   Depositor

   Roee Amit

   Article

   Katz et al Nat Commun. 2021 Mar 11;12(1):1576. doi:

   Insert

   10 binding sites of PP7 from the oligo library
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAS156

   Plasmid

   #194479

   Purpose

   synER Part (Part 3)

   Depositor

   Ahmad Khalil

   Article

   Sanford et al ACS Synth Biol. 2022 Nov 11. doi: 10.102

   Insert

   synER (Part 3)
   

   Use

   Synthetic Biology

   Available Since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pscMS2(WTx2)-EGFP

   Plasmid

   #140258

   Purpose

   MS2CP-dependent translational repression

   Depositor

   Hirohide Saito

   Article

   Ono et al ACS Synth Biol. 2020 Jan 17;9(1):169-174

   Insert

   Two MS2CP-binding sites embedded in scaffold
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. UBQ10:sXVE:(MCS)-3xHA-S11

   Plasmid

   #108180

   Purpose

   Binary vectors used for the inducible expression of tripartite split-sfGFP components (with a 3xHA tag) in plants

   Depositor

   Tzu-Yin Liu

   Article

   Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Plant

   Promoter

   UBQ10:sXVE:

   Available Since

   April 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. UBQ10:sXVE:S10-(MCS)-3xHA

   Plasmid

   #108178

   Purpose

   Binary vectors used for the inducible expression of tripartite split-sfGFP components (with a 3xHA tag) in plants

   Depositor

   Tzu-Yin Liu

   Article

   Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Plant

   Promoter

   UBQ10:sXVE:

   Available Since

   May 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. p168

    Plasmid

    #62618

    Purpose

    ePathBrick pETM6 vector encoding CsF3H, FaDFR, and DuLAR

    Depositor

    Mattheos Koffas

    Article

    Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1

    Inserts

    flavaone 3B-hydroxylase
    dihydroflavonol 4-reductase
    leucoanthocyanidin reductase
    

    Use

    Synthetic Biology

    Tags

    GBD, PDZ, and SH3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGTag-eGFP-CAAX-B-actin

    Plasmid

    #117816

    Purpose

    Donor for precise CRISPR directed genomic integration using the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

    Insert

    eGFP
    

    Use

    Synthetic Biology

    Tags

    CAAX

    Available Since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-REPORT(PGK)

    Plasmid

    #172328

    Purpose

    LV REPORT containing minimal promoter driving monomeric nuclear Tomato 2a HSVtk 2a Neo followed by an PGK promoter driving nuclear d2eGFP E2A Hygromycin

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    monomeric nuclear Tomato
    d2eGFP
    HygR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3x SV40 NLS

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. LSB-hsa-miR-29a-5p

    Plasmid

    #103392

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-29a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-29a-5p target (MIR29A Human)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. DVK_AF

    Plasmid

    #66068

    Purpose

    MoClo Destination Vector: (based on pSB1K3) [A:LacZa:F]

    Depositor

    Douglas Densmore

    Article

    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

    Type

    Empty backbone

    Use

    Synthetic Biology; Destination vector

    Available Since

    Jan. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

15. p4xSMAD_RE

    Plasmid

    #118051

    Purpose

    GBPart - Operator/DNA Response element - 4 copies the SMAD DNA binding motif

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Insert

    4 copies the SMAD DNA binding motif
    

    Use

    Synthetic Biology; Domestication of dna parts for…

    Expression

    Mammalian

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEPYC0CM0410

    Plasmid

    #154567

    Purpose

    Phytobricks Level 0 part

    Depositor

    Nicola Patron

    Article

    Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

    Insert

    MinSyn_104
    

    Use

    Synthetic Biology

    Available Since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEPYC0CM0414

    Plasmid

    #154571

    Purpose

    Phytobricks Level 0 part

    Depositor

    Nicola Patron

    Article

    Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

    Insert

    MinSyn_108
    

    Use

    Synthetic Biology

    Available Since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAV105

    Plasmid

    #63195

    Purpose

    pRS405 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. Construct 33 - NFKBRp_Cas9_3xNLS_p2a-puroR

    Plasmid

    #81255

    Purpose

    Lentiviral construct for building stable cell lines expressing Cas9 in the presence of TNFa via puromycin selection

    Depositor

    Timothy Lu

    Article

    Perli et al Science. 2016 Aug 18. pii: aag0511.

    Insert

    Cas9-3xNLS
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBTK1003 (pSL2)

    Plasmid

    #190998

    Purpose

    Expresses red chromoprotein on a broad host range RSF1010 origin plasmid

    Depositor

    Jeffrey Barrick

    Article

    Elston et al ISME Commun. 2023 May 24;3(1):49. doi: 1

    Insert

    red chromoprotein
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    CP25

    Available Since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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