We narrowed to 9,766 results for: 158

1. pEJS-HZ54 All-in-one AAV-U1a-NmeABE8e-2xBPSV40-U6-Rosa26

   Plasmid

   #199265

   Purpose

   Single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one U6 driven sgRNA targeting mouse Rosa26 gene

   Depositor

   Erik Sontheimer

   Article

   Zhang et al GEN Biotechnol. 2022 Jun 1;1(3):285-299.

   Insert

   NmeABE8e
   

   Use

   AAV and CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   U1a

   Available since

   April 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-TA-ERP2-hcGAS(wt)

   Plasmid

   #208396

   Purpose

   To generate stable cell line expressing human cGAS(wt) using PiggyBac transposon system

   Depositor

   Gaorav Gupta

   Article

   Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

   Insert

   Human cGAS (CGAS Human)
   

   Use

   Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB-TA-ERP2-hcGAS(R255A)

   Plasmid

   #208397

   Purpose

   To generate stable cell line expressing human cGAS(R255A) using PiggyBac transposon system

   Depositor

   Gaorav Gupta

   Article

   Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

   Insert

   Human cGAS (CGAS Human)
   

   Use

   Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   R255A

   Promoter

   Available since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEGFP-N3-CYB5R3

   Plasmid

   #204567

   Purpose

   Expresses EGFP-tagged CYB5R3 in mammalian cells

   Depositor

   Masaaki Komatsu

   Article

   Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

   Insert

   CYB5R3 (CYB5R3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   Available since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. phBG-P53DD

   Plasmid

   #149707

   Purpose

   In vitro transcription of dominant negative form of P53 mRNA for RNA transfection into mammalian cells

   Depositor

   Erika Sasaki

   Article

   Watanabe et al Genes Cells. 2019 Jul;24(7):473-484. doi

   Insert

   Trp53 (Trp53 Mouse)
   

   Use

   Rna transcription

   Tags

   Expression

   Mutation

   deleted amino acid 14-301

   Promoter

   T7

   Available since

   June 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA-LmoCas6-p300

   Plasmid

   #126486

   Purpose

   Encodes L.monocytogenes CRISPR Type I-B Cas6 with N-terminal fusion of Flag epitope and NLS and C-terminal fusion of NLS, human p300 HAT core (aa1048-1664), and Flag tag epitope driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

   Insert

   LmoCas6-p300
   

   Use

   CRISPR

   Tags

   Flag and Flag, NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEJS1830 All-in-one AAV-U1a-NmeABE-8e-2xBPSV40-U6-Fah

   Plasmid

   #199263

   Purpose

   Single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one U6 driven sgRNA targeting the point mutation in the mouse Fah gene of a HT1 mouse model

   Depositor

   Erik Sontheimer

   Article

   Zhang et al GEN Biotechnol. 2022 Jun 1;1(3):285-299.

   Insert

   NmeABE8e
   

   Use

   AAV and CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   U1a

   Available since

   April 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. ACTB-(A)3x

   Plasmid

   #112057

   Purpose

   ACTB mRNA tagged with 3 copies of Riboglow (variant A)

   Depositor

   Amy Palmer

   Article

   Braselmann et al Nat Chem Biol. 2018 Jul 30. pii: 10.1038

   Insert

   ACTB (ACTB Human)
   

   Use

   Tags

   Riboglow RNA tag

   Expression

   Mammalian

   Mutation

   3 copies of Riboglow tag (variant A) after stop c…

   Promoter

   CMV

   Available since

   June 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. ACTB-(A)2x

   Plasmid

   #112056

   Purpose

   ACTB mRNA tagged with 2 copies of Riboglow (variant A)

   Depositor

   Amy Palmer

   Article

   Braselmann et al Nat Chem Biol. 2018 Jul 30. pii: 10.1038

   Insert

   ACTB (ACTB Human)
   

   Use

   Tags

   Riboglow RNA tag

   Expression

   Mammalian

   Mutation

   2 copies of Riboglow tag (variant A) after stop c…

   Promoter

   CMV

   Available since

   June 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. NC4 pCDNA3.1 VENUS WTX (1-804)

    Plasmid

    #36959

    Depositor

    Randall Moon

    Article

    Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

    Insert

    WTX (AMER1 Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pUC19-U6-MbCas12a crRNA-BsmBI cassette (BPK4449)

    Plasmid

    #114088

    Purpose

    U6 promoter crRNA entry vector used for all MbCas12a crRNAs (clone spacer oligos into BsmBI cassette)

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    entry vector for MbCas12a crRNAs
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-Flag-Avi-ires-CHD5

    Plasmid

    #68878

    Purpose

    Transient mammalian expression of CHD5 for BirA tagging

    Depositor

    Adrian Bracken

    Article

    Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

    Insert

    CHD5 (CHD5 Human)
    

    Use

    Tags

    FLAG/AVI

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

13. Lenti U6-HBG site1-EF1alpha-UGI-P2A-GFP

    Plasmid

    #157951

    Purpose

    Lentiviral expression of HBG site 1 sgRNA

    Depositor

    Dali Li

    Article

    Zhang et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    Lenti U6-HBG site1-EF1alpha-UGI-P2A-GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA-CasE-p300-Flag

    Plasmid

    #106275

    Purpose

    Encodes E.coli CRISPR Type I-E CasE with N-terminal fusion of E tag epitope and NLS and C-terminal fusion of NLS, human p300 HAT core (aa1048-1664), and Flag tag epitope driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    EcoCasE-p300
    

    Use

    CRISPR

    Tags

    E tag, NLS and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1/Xpress beta4 1355T

    Plasmid

    #16040

    Depositor

    Filippo Giancotti

    Article

    Nikolopoulos et al Mol Cell Biol. 2005 Jul . 25(14):6090-10

    Insert

    beta4 (ITGB4 Human)
    

    Use

    Tags

    6xHis and Xpress

    Expression

    Mammalian

    Mutation

    1355T: amino acids 1-1355

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pGEX-GST-CHD5(Chromos)-FRAG

    Plasmid

    #68875

    Purpose

    IPTG inducible bacterial expression of CHD5 fragment

    Depositor

    Adrian Bracken

    Article

    Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

    Insert

    CHD5 (CHD5 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    only the two chromodomains of CHD5

    Promoter

    tac

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pCMV-FLAG-CHD5-WT-331-672aa

    Plasmid

    #68870

    Purpose

    Mammalian expression of CHD5 histone binding domain (331-672aa)

    Depositor

    Adrian Bracken

    Article

    Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

    Insert

    CHD5 (CHD5 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    331-672aa

    Promoter

    CMV

    Available since

    Nov. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pFRT-TO-FLAG(N)-CDC73

    Plasmid

    #231751

    Purpose

    Expression in Flp-IN human cells

    Depositor

    Jesper Svejstrup

    Article

    Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

    Insert

    CDC73 (CDC73 Human)
    

    Use

    Tags

    Flag-3C

    Expression

    Mammalian

    Mutation

    siCDC73#6 and #8 resistant

    Promoter

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFRT-TO-FLAG(N)-CDC73deltaKIM

    Plasmid

    #231752

    Purpose

    Expression in Flp-IN human cells

    Depositor

    Jesper Svejstrup

    Article

    Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

    Insert

    CDC73 (CDC73 Human)
    

    Use

    Tags

    Flag-3C

    Expression

    Mammalian

    Mutation

    siCDC73#6 and #8 resistant; CDC73(delta290-324)

    Promoter

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFRT-TO-FLAG(N)-CDC73-YY/AA

    Plasmid

    #231753

    Purpose

    Expression in Flp-IN human cells

    Depositor

    Jesper Svejstrup

    Article

    Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

    Insert

    CDC73 (CDC73 Human)
    

    Use

    Tags

    Flag-3C

    Expression

    Mammalian

    Mutation

    siCDC73#6 and #8 resistant; CDC73(Y290A, Y293A)

    Promoter

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLIB-FLAG(N)-CDK12deltaNTD(700-1490)

    Plasmid

    #231734

    Purpose

    Protein expression in insect cells

    Depositor

    Jesper Svejstrup

    Article

    Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

    Insert

    CDK12 (CDK12 Human)
    

    Use

    Tags

    Flag-3C

    Expression

    Insect

    Mutation

    CDK12(700-1490)

    Promoter

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLIB-FLAG(N)-CDK12-KiD(700-1082)

    Plasmid

    #231736

    Purpose

    Protein expression in insect cells

    Depositor

    Jesper Svejstrup

    Article

    Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

    Insert

    CDK12 (CDK12 Human)
    

    Use

    Tags

    Flag-3C

    Expression

    Insect

    Mutation

    CDK12(700-1082)

    Promoter

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLIB-FLAG(N)-CDK12deltaRS(delta104-380)

    Plasmid

    #231737

    Purpose

    Protein expression in insect cells

    Depositor

    Jesper Svejstrup

    Article

    Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

    Insert

    CDK12 (CDK12 Human)
    

    Use

    Tags

    Flag-3C

    Expression

    Insect

    Mutation

    CDK12(delta104-380)

    Promoter

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLIB-FLAG-GST(N)-CDK12RS(104-380)

    Plasmid

    #231738

    Purpose

    Protein expression in insect cells

    Depositor

    Jesper Svejstrup

    Article

    Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

    Insert

    CDK12 (CDK12 Human)
    

    Use

    Tags

    Flag-GST-3C

    Expression

    Insect

    Mutation

    CDK12(104-380)

    Promoter

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLIB-FLAG(N)-CDC73deltaKIM(delta290-324)

    Plasmid

    #231739

    Purpose

    Protein expression in insect cells

    Depositor

    Jesper Svejstrup

    Article

    Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

    Insert

    CDC73 (CDC73 Human)
    

    Use

    Tags

    Flag-3C

    Expression

    Insect

    Mutation

    CDC73(delta290-324)

    Promoter

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLIB-FLAG(N)-CDC73-YY/AA(Y290A, Y293A)

    Plasmid

    #231740

    Purpose

    Protein expression in insect cells

    Depositor

    Jesper Svejstrup

    Article

    Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

    Insert

    CDC73 (CDC73 Human)
    

    Use

    Tags

    Flag-3C

    Expression

    Insect

    Mutation

    CDC73(Y290A, Y293A)

    Promoter

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. sgYAP-R-1

    Plasmid

    #229445

    Purpose

    knockout of YAP1

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    sgYAP-R-1 (YAP1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6;bU6;EFS

    Available since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCS2+ E4F1 degron(220-242)-GFP-IRES-mCherry

    Plasmid

    #231007

    Purpose

    Protein stability reporter construct for E4F1 consisting of aa 220-242 for transient overexpression in mammalian cells.

    Depositor

    Michael Rape

    Article

    Manford et al Cell. 2021 Oct 14;184(21):5375-5390.e16.

    Insert

    E4F1 aa220-242 (E4F1 Human)
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. HA-MST1-7A

    Plasmid

    #229418

    Purpose

    Lentiviral or overpexression of cDNA

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    MST1-7A (MST1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    MARK2 phosphosites (T175,T273,S320,S327,T340,T387…

    Promoter

    EFS

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. sgYAP-R-2

    Plasmid

    #229446

    Purpose

    knockout of YAP1

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    sgYAP-R-2 (YAP1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6;bU6;EFS

    Available since

    Jan. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge