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Showing: 5681 - 5720 of 8357 results

  1. pMBPcyto-TEV-BseRI

    Plasmid
    #157739
    Purpose
    empty vector adds TEV-protease-removable N-term His10 + maltose binding protein (without signal sequence); avoids unwanted residues in the final protein sequence by using the BseRI restriction sites
    Depositor
    Debra Hansen
    Type
    Empty backbone
    Use
    Tags
    His10 + MBP (cytoplasmic expression; no signal se…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    157739-plasmid-map-sequence-id-334003

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  2. CD44MMA-c002

    Plasmid
    #175452
    Purpose
    Protein expression in bacterial cells (as inclusion bodies). CD44 Link module, Q23-D172. Used for crystallography.
    Depositor
    Opher Gileadi
    Insert
    Cd44 (Cd44 Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175452-plasmid-map-sequence-id-353153

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  3. ALDH7A1

    Plasmid
    #38866
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ALDH7A1 (Aldh7a1 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  4. pJK372

    Plasmid
    #73597
    Purpose
    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-15 fatty acid desaturase (FAD3) with C-terminal c-myc epitope
    Depositor
    T. J. Kappock
    Inserts
    delta-12 fatty acid acetylenase
    delta-15 fatty acid desaturase
    Use
    Tags
    FLAG tag and c-myc tag
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 and GAL10
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    73597-plasmid-map-sequence-id-138754

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  5. pGB-08-09-BECN1

    Plasmid
    #187991
    Purpose
    Plasmid for the protein expression of BECN1/Beclin1 in a bacterial and insect expression system. Internal_Reference: SMC1325
    Depositor
    Sascha Martens
    Insert
    BECN1 (BECN1 Human)
    Use
    Tags
    Expression
    Bacterial and Insect
    Mutation
    Promoter
    Available since
    Feb. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    187991-plasmid-map-sequence-id-374168

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  6. DAPK3

    Plasmid
    #38964
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    DAPK3 (DAPK3 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  7. 4LG7

    Plasmid
    #60094
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    MBD4:JMC02A-C01:C228095 (MBD4 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  8. DIRAS2

    Plasmid
    #38819
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    DIRAS2 (diras2 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  9. pJK370

    Plasmid
    #73595
    Purpose
    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-2) with C-terminal c-myc epitope
    Depositor
    T. J. Kappock
    Inserts
    delta-12 fatty acid acetylenase
    delta-12 fatty acid desaturase (form 2)
    Use
    Tags
    FLAG tag and c-myc tag
    Expression
    Yeast
    Mutation
    Silent mutation in Arg299 codon.
    Promoter
    GAL1 and GAL10
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    73595-plasmid-map-sequence-id-138462

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  10. PCTK1

    Plasmid
    #39053
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PCTK1 (CDK16 Human)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Activation loop mutant S319D
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  11. pET28a-HIS-TagGFP2-TEV-GGG-ELP(40nm)-Cys

    Plasmid
    #90474
    Purpose
    Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]2, with N-Terminal TagGFP2 and TEV-cleavage site, leaving an N-terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine
    Depositor
    Hermann Gaub
    Insert
    TagGFP2-TEV-GGG-ELP(40nm)-Cys
    Use
    Tags
    Cysteine and Sortase-Tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    90474-plasmid-map-sequence-id-181091

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  12. isoform PKC Beta (JCE694)

    Plasmid
    #182024
    Purpose
    yeast expression of mouse PKC Beta with yeast PKC1 promoter and ADH1 terminator
    Depositor
    Juan Carlos Igual
    Insert
    PKCBeta (Prkcb Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    yeast PKC1 promoter (-600 to -1)
    Available since
    May 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    182024-plasmid-map-sequence-id-362004

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  13. HOXD8 (human,partial) HIS-tag pET

    Plasmid
    #8569
    Depositor
    Corey Largman
    Insert
    HOXD8 (HOXD8 Human)
    Use
    Tags
    His and T7
    Expression
    Bacterial
    Mutation
    Partial protein containing 4 AA N-terminal to HD …
    Promoter
    Available since
    May 30, 2006
    Availability
    Academic Institutions and Nonprofits only
    8569-plasmid-map-sequence-id-387145

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  14. CA7

    Plasmid
    #39050
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CA7 (CA7 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  15. ACAD11

    Plasmid
    #39004
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ACAD11 (ACAD11 Human)
    Use
    Tags
    His6-Thioredoxin-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  16. GPX8

    Plasmid
    #38917
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GPX8 (GPX8 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  17. ANLN

    Plasmid
    #39088
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ANLN (ANLN Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  18. BRWD1

    Plasmid
    #39073
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    BRWD1 (BRWD1 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  19. PHF20 (3QII)

    Plasmid
    #32039
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PHF20 (PHF20 Human)
    Use
    Tags
    His (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    Tudor domain 2 only (amino acids 83-150)
    Promoter
    T7
    Available since
    Aug. 8, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  20. pT7-Venus-cdc25C1

    Plasmid
    #40968
    Depositor
    Olivier Gavet
    Insert
    cdc25C5 (CDC25C Human)
    Use
    In vitro transcription
    Tags
    Venus
    Expression
    Mutation
    Promoter
    T7
    Available since
    Feb. 12, 2015
    Availability
    Academic Institutions and Nonprofits only

  22. pcDNA3.1/hpa5

    Plasmid
    #59972
    Purpose
    integrin a5 wild-type headpiece expression (a5 beta-propeller and a5 thigh domain)
    Depositor
    Timothy Springer
    Insert
    human integrin a5 headpiece (ITGA5 Human)
    Use
    Tags
    acid coiled-coil and StrepII tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    Oct. 23, 2014
    Availability
    Academic Institutions and Nonprofits only

  23. RFXANK (3V3O)

    Plasmid
    #36905
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RFXANK (RFXANK Human)
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    amino acid residues 90-260 (ANK repeats)
    Promoter
    Available since
    June 19, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  24. GPX7

    Plasmid
    #38791
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GPX7 (GPX7 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  25. LNX2

    Plasmid
    #38920
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    LNX2 (LNX2 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  26. pLV-Enh eGFP Reporter-muHRNR-X50

    Plasmid
    #59451
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of mouse HMR.
    Depositor
    Benjamin Yu
    Insert
    Hrnr (Hrnr Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Mu Hsp68 minimal promoter
    Available since
    Oct. 24, 2014
    Availability
    Academic Institutions and Nonprofits only

  27. pUAST-FOXO 3A

    Plasmid
    #52983
    Purpose
    insect expression of FOXO triple mutant
    Depositor
    Marc Montminy, Biao Wang
    Insert
    FOXO (foxo Fly)
    Use
    Tags
    3x HA
    Expression
    Insect
    Mutation
    3A, triple mutant, T44A S190A S259A
    Promoter
    Hsp70
    Available since
    May 20, 2014
    Availability
    Academic Institutions and Nonprofits only

  28. pGEX-6P-1-GST-CD-ATL (Cbx7)

    Plasmid
    #82527
    Purpose
    Expresses mutant GST-CD-ATL (Cbx7) fusion proteins in E. coli. Cbx7 CD-ATL region, amino acids 1–84.
    Depositor
    Xiaojun Ren
    Insert
    Chromobox Homolog 7 (CBX7 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    amino acids 1–84
    Promoter
    Available since
    Dec. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    82527-plasmid-map-sequence-id-156822

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  29. GLRX3

    Plasmid
    #39090
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GLRX3 (GLRX3 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  30. PSD-95_Y12E-pTagRFP

    Plasmid
    #52672
    Purpose
    expression of TagRFP-tagged PSD-95_Y12E
    Depositor
    Johannes Hell
    Insert
    PSD-95 (Dlg4 Rat)
    Use
    Tags
    tagRFP
    Expression
    Mammalian
    Mutation
    Y12E
    Promoter
    CMV
    Available since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    52672-plasmid-map-sequence-id-81956

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  31. JMC065-G11

    Plasmid
    #58762
    Purpose
    Baculovirus expression for structure determination; may not be full ORF. PDB ID: 4PXW
    Depositor
    Cheryl Arrowsmith
    Insert
    PDB:4PXW (DCAF1 Human)
    Use
    Baculovirus expression
    Tags
    His
    Expression
    Mutation
    Promoter
    polyhedrin promoter
    Available since
    Aug. 4, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  32. JMC02A-C10

    Plasmid
    #58754
    Purpose
    bacterial expression of PDB 4O61
    Depositor
    Cheryl Arrowsmith
    Insert
    ALKBH5 (ALKBH5 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Bacterial expression for structure determination;…
    Promoter
    T7
    Available since
    Aug. 4, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  33. pMSCV_puro_41585

    Plasmid
    #111631
    Purpose
    Complementation of Dicer_KO mESCs with mutated human DICER isoform
    Depositor
    Constance Ciaudo
    Insert
    FLAG-hsDicer (D1320A/D1709A) (DICER1 Human)
    Use
    Retroviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    D1320A/D1709A
    Promoter
    Available since
    Aug. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    111631-plasmid-map-sequence-id-217672

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  34. BC-Sig-GPRC

    Plasmid
    #44201
    Depositor
    Lei Xu
    Insert
    human GPR56 C-terminal fragment with signal peptide (ADGRG1 Human)
    Use
    Retroviral
    Tags
    none
    Expression
    Mammalian
    Mutation
    The C-terminal fragment of GPR56, starting at am…
    Promoter
    LTR
    Available since
    July 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    44201-plasmid-map-sequence-id-60060

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  35. CUL4B

    Plasmid
    #39108
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CUL4B (CUL4B Human)
    Use
    Tags
    His6-Zb-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  37. GMDS

    Plasmid
    #39152
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GMDS (GMDS Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  38. pTRIPZ (M)-HT-CD(Cbx7)

    Plasmid
    #82520
    Purpose
    Lentivirus vector. Expresess mutant Halotag-CD-Cbx7 fusion proteins in mammalian cells. Express Chromodomain of Cbx7; amino acids 8–62.
    Depositor
    Xiaojun Ren
    Insert
    Chromobox Homolog 7 (CBX7 Human)
    Use
    Lentiviral
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    amino acids 8–62
    Promoter
    CMV
    Available since
    Jan. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    82520-plasmid-map-sequence-id-170982

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Showing: 5681 - 5720 of 8357 results