We narrowed to 8,780 results for: construct

Showing: 5681 - 5720 of 8780 results

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pJB01

Plasmid

#111957

Purpose

Expresses His10-RFP-Halo; construct generated for pull-down and localization experiments

Depositor

Matthew Good

Article

Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

Insert

RFP

Tags

HaloTag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV hSynapsin flexed SV-tag

Plasmid

#163686

Purpose

express SV-Tag construct in a Cre-dependent manner

Depositor

Bernardo Sabatini

Article

Chantranupong et al Elife. 2020 Oct 12;9. pii: 59699. doi: 1

Insert

synaptophysin 9X HA

Use

AAV

Tags

9X HA

Expression

Mammalian

Promoter

synapsin

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pAE03

Plasmid

#61044

Purpose

To construct C-terminal GFP fusions, single crossover integration vector

Depositor

Jan-Willem Veening

Article

Eberhardt et al Mol Microbiol. 2009 Oct;74(2):395-408. d

Type

Empty backbone

Use

Synthetic Biology

Tags

GFP+

Expression

Bacterial

Available Since

Feb. 6, 2015

Availability

Academic Institutions and Nonprofits only

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PEP111E

Plasmid

#97397

Purpose

binary construct of plastid-targeted mCherry with sfGFP11 tag for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

mCherry-sfGFP11

Tags

Plastid target

Expression

Plant

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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TALED_Right-stuffer-1397N

Plasmid

#187172

Purpose

original vector to make all constructs

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

Insert

Right-stuffer-1397N

Expression

Mammalian

Promoter

pCMV

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-IL12-26Sfur-B2AD-tvmvs-KKMP

Plasmid

#179646

Purpose

TVMVP-inducible IL-12 RELEASE construct

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

IL12 A/B

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-StrepII-TagRFP-CohI

Plasmid

#58710

Purpose

Detection construct to label dockerin type I containing fusion proteins

Depositor

Hermann Gaub

Article

Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

Insert

TagRFP-CohI

Tags

StrepII

Expression

Bacterial

Promoter

T7

Available Since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

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SPDK3181

Plasmid

#97403

Purpose

binary construct of ER-targeted sfCherry1-10 for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

ER-sfCherry1-10

Tags

ER target

Expression

Plant

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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SPDK3182

Plasmid

#97404

Purpose

binary construct of ER-targeted sfYFP1-10 for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

ER-sfYFP1-10

Tags

ER target

Expression

Plant

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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SPDK3184

Plasmid

#97405

Purpose

binary construct of cytosolic sfCFP1-10 tag for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

sfCFP1-10

Tags

cytosol

Expression

Plant

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pMS17: pTwinStrep-SUMO-AvTnsD

Plasmid

#168150

Purpose

AvTnsD expression construct for protein purification.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Insert

AvCAST TnsD

Tags

6X His, Twin Strep and SUMO

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pMS15: pTwinStrep-SUMO-AvTnsB

Plasmid

#168148

Purpose

AvTnsB expression construct for protein purification.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Insert

AvCAST TnsB

Tags

6X His, Twin Strep and SUMO

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pMS16: pTwinStrep-SUMO-AvTnsC

Plasmid

#168149

Purpose

AvTnsC expression construct for protein purification.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Insert

AvCAST TnsC

Tags

6X His, Twin Strep and SUMO

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pMS14: pTwinStrep-SUMO-AvTnsA

Plasmid

#168147

Purpose

AvTnsA expression construct for protein purification.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Insert

AvCAST TnsA

Tags

6X His, Twin Strep and SUMO

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCB-CBEv1

Plasmid

#221138

Purpose

Coxiella burnetii CRISPR-Cas9 cytosine base editing plasmid version 1 without sgRNA construct. Expresses 3xF-HF-BE3.

Depositor

Craig Roy

Article

Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

Insert

3xF-rAPOBEC1-Cas9n(HF)-UGI (HF-BE3)

Use

CRISPR

Tags

3xFLAG

Expression

Bacterial

Promoter

lacIq-Ptac

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pYC34

Plasmid

#63915

Purpose

Recipient overexpressing vector to construct mCherry tagged fusions

Depositor

Irene Castano Navarro

Article

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Tags

mCherry

Expression

Yeast

Promoter

TEF1 (Saccharomyces cerevisiae)

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only

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PEP106E

Plasmid

#97392

Purpose

binary construct of ER-targeted sfGFP1-10 for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

sfGFP1-10OPT

Tags

ER target

Expression

Plant

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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PEP102E

Plasmid

#97388

Purpose

binary construct of nuclear-targeted sfGFP1-10 for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

sfGFP1-10OPT

Tags

Nuclear target

Expression

Plant

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pYC46

Plasmid

#63904

Purpose

Recipient integrative vector to construct FLAG tagged fusions

Depositor

Irene Castano Navarro

Article

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Tags

FLAG

Expression

Yeast

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only

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PEP107E

Plasmid

#97393

Purpose

binary construct of Golgi-targeted sfGFP1-10 for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

sfGFP1-10OPT

Tags

Golgi target

Expression

Plant

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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JAB2296

Plasmid

#190365

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_pro35S-PhlFO::GFPint-NLS-ADHt)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_pro35S-PhlFO::GFPint-NLS-ADHt

Expression

Plant

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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PEP113E

Plasmid

#97399

Purpose

binary construct of peroxisome-targeted mCherry with sfGFP11 tag for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

mCherry-sfGFP11

Tags

Peroxisome target

Expression

Plant

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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PEP114E

Plasmid

#97400

Purpose

binary construct of ER-targeted mCherry with sfGFP11 tag for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

mCherry-sfGFP11

Tags

ER target

Expression

Plant

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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plIVMD156

Plasmid

#160934

Purpose

In vivo mRNA display Gateway Destination construct

Depositor

Saeed Tavazoie

Article

Oikonomou et al Proc Natl Acad Sci U S A. 2020 Oct 9. pi

Insert

MCP-Gateway cloning site-HIS tag, 3'UTR MS2 Stem Loop

Tags

HIS tag, 3'UTR MS2 Stem Loop

Expression

Yeast

Promoter

MET25

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pMDR1-221

Plasmid

#69481

Purpose

Deletion Construct

Depositor

Kathleen Scotto

Article

Jin et al Mol Cell Biol. 1998 Jul;18(7):4377-84.

Insert

MDR1 promoter (ABCB1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Insert includes MDR1 promoter sequence from -221 …

Available Since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only
pAAV syn CCR6-eLOV-TEVcs-tTA

Plasmid

#173116

Purpose

Expresses CCR6 receiver construct in neurons

Depositor

Alice Ting

Article

Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

Insert

CCR6-eLOV-TEVcs-tTA

Use

AAV

Promoter

Synapsin

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pNP151

Plasmid

#106359

Purpose

To insert a single copy of a construct at a site on Chr II, corresponding to a mKate-labelled LifeAct expressed specifically in the adult epidermis.

Depositor

Jonathan Ewbank

Article

Taffoni et al Elife. 2020 Jan 29;9. pii: 45047. doi: 1

Insert

col-62p_lifeact_mkate2_3'nmy-2

Expression

Worm

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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PEP103E

Plasmid

#97389

Purpose

binary construct of plastid-targeted sfGFP1-10 for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

sfGFP1-10OPT

Tags

Plastid target

Expression

Plant

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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PEP105E

Plasmid

#97391

Purpose

binary construct of peroxisome-targeted sfGFP1-10 for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

sfGFP1-10OPT

Tags

Peroxisome target

Expression

Plant

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
JAB3312

Plasmid

#190406

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_pro35S-AmtRO-PhlFO::GFPint-NLS-ADHt)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_pro35S-AmtRO-PhlFO::GFPint-NLS-ADHt

Expression

Plant

Available Since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
JAB3603

Plasmid

#190422

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_4XPhlFO::GFPint-ADHt)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_4XPhlFO::GFPint-ADHt

Expression

Plant

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
JAB2493

Plasmid

#190375

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_6XLacIO-min35S::GFPint-ADHt)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_6XLacIO-min35S::GFPint-ADHt

Expression

Plant

Available Since

April 4, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
JAB2192

Plasmid

#190356

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_6XQacRO-min35S::GFPint-ADHt)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_6XQacRO-min35S::GFPint-ADHt

Expression

Plant

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
JAB2191

Plasmid

#190355

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_6XLmrAO-min35S::GFPint-ADHt)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_6XLmrAO-min35S::GFPint-ADHt

Expression

Plant

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC-DEST-R3R4(R)

Plasmid

#141010

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Type

Empty backbone

Expression

Mammalian

Available Since

July 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-α5last

Plasmid

#160270

Purpose

Expresses a concatemeric construct of a α4:β2:α5 (2:2:1) nAChR

Depositor

Pierre-Jean Corringer

Article

Prevost et al Cell Mol Life Sci. 2020 May 29. pii: 10.

Insert

α5last concatemer

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5-α5first

Plasmid

#160269

Purpose

Expresses a concatemeric construct of a α4:β2:α5 (2:2:1) nAChR

Depositor

Pierre-Jean Corringer

Article

Prevost et al Cell Mol Life Sci. 2020 May 29. pii: 10.

Insert

α5first concatemer

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pADH139

Plasmid

#90987

Purpose

NAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR; use with pADH110 to generate target-specific part 2 of 2 for C.mal LEUpOUT CRISPR system.

Depositor

Aaron Hernday

Article

Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

Insert

gRNA conserved, C, mal LEU2 2 of 2

Use

CRISPR

Available Since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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pUC-DEST-R1R2

Plasmid

#141056

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Type

Empty backbone

Expression

Mammalian

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pYC54

Plasmid

#63909

Purpose

Recipient integrative vector to construct YFP tagged fusions

Depositor

Irene Castano Navarro

Article

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Tags

YFP

Expression

Yeast

Available Since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge

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