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pcDNA5/FRT/TO/intron/FlagCter

Plasmid

#113548

Purpose

Mammalian expression plasmid that can be used with the Flp-In T-REx system.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

Flag

Tags

Flag

Expression

Mammalian

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pcihygrosynDAT

Plasmid

#19992

Depositor

Jonathan Javitch

Article

Saunders et al Proc Natl Acad Sci U S A. 2000 Jun 6. 97

Insert

synDAT (SLC6A3 Human)

Expression

Mammalian

Available Since

Sept. 25, 2009

Availability

Academic Institutions and Nonprofits only

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pCDNA3_PKA-Cα(K8,9A)-mPAGFP

Plasmid

#168493

Purpose

Mammalian expression of the K8,9A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.

Depositor

Haining Zhong

Article

Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11

Insert

the K8,9A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP

Expression

Mammalian

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pMGF173

Plasmid

#96999

Purpose

NLS-GFP-lacZ leu1 insertion vector

Depositor

Adam Frost, Wesley Sundquist

Article

Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

Insert

lacZ

Tags

NLS-GFP

Expression

Yeast

Promoter

Pnmt1

Available Since

Aug. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pLLX8

Plasmid

#79838

Purpose

Contains yeast counter selectable marker, CYH2, and ampicillin resistance cassette, used for making a DNA capture vector

Depositor

Cammie Lesser, Stephen Lory

Article

Wolfgang et al Proc Natl Acad Sci U S A. 2003 Jul 8;100

Type

Empty backbone

Use

Contains yeast counter selection gene, cyh2 and b…

Available Since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pMK1224

Plasmid

#84221

Purpose

Lentiviral expression of shRNAs, compatible with double-shRNA expression, SFFV promoter, BFP

Depositor

Martin Kampmann, Jonathan Weissman

Article

Kampmann et al Proc Natl Acad Sci U S A. 2015 Jun 30;11

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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vector plix 4.03

Plasmid

#121130

Purpose

inducible expression in cells

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

empty cassete for contol of miR expression

Expression

Bacterial

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pDisplay FLIPE-100u

Plasmid

#13547

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E S118W

Tags

ECFP and Venus

Expression

Mammalian

Mutation

S118W in binding protein

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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rGSKmyc-pXT7

Plasmid

#15492

Depositor

Sergei Sokol

Article

Dominguez et al Proc Natl Acad Sci U S A. 1995 Aug 29. 9

Insert

GSK-3 beta (Gsk3b Rat)

Use

Xenopus rna expression

Tags

myc

Available Since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
pCDNA3_PKA-CαN15-mVenus

Plasmid

#168486

Purpose

Mammalian expression of the N-terminal 15 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.

Depositor

Haining Zhong

Article

Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11

Insert

the N-terminal 15 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus

Expression

Mammalian

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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nPEX-LV

Plasmid

#12119

Depositor

Inder Verma

Article

Pfeifer et al Proc Natl Acad Sci U S A. 2000 Oct 24. 9

Insert

Matrix Metalloproteinase 2 (Mmp2 Mouse)

Use

Lentiviral

Mutation

noncatalytic fragment of MMP2 amplified via PCR, …

Available Since

April 27, 2007

Availability

Academic Institutions and Nonprofits only
psicheck-2-Sfxn3

Plasmid

#48169

Purpose

contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element

Depositor

Vamsi Mootha

Article

Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

Insert

5" UTR of SFXN3 (SFXN3 Human)

Use

Luciferase

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only
pET:CCCT:GFP

Plasmid

#60738

Purpose

A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'CCCT' between -11 and -8.

Depositor

Matthew Bennett

Article

Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pAG416GPD-Cerulean-CHC1

Plasmid

#18850

Depositor

Susan Lindquist

Article

Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

Insert

CHC1 (CHC1 Budding Yeast)

Tags

Cerulean

Expression

Yeast

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
TU#1450_unc-53a_cDNA

Plasmid

#82361

Purpose

express unc-53a cDNA

Depositor

Martin Chalfie

Article

Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

unc-53 a isoform (unc-53 Nematode)

Expression

Worm

Promoter

none

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pBW216J106-Amp32C

Plasmid

#78651

Purpose

pSB3K3 carrying J106-30hrpR-32hrpS-t-hrpL-30gfp-tT

Depositor

Baojun Wang

Article

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

J106-30hrpR-32hrpS-t-hrpL-30gfp-t

Use

Synthetic Biology

Promoter

see insert

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only
pPS833

Plasmid

#1358

Depositor

Pamela Silver

Article

Schlenstedt et al Proc Natl Acad Sci U S A 1995 Jan 3;92(1

Insert

GSP1 (GSP1 Budding Yeast)

Expression

Yeast

Mutation

G21V, stabilizes the GTP-bound form

Available Since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
FCIV1-GR S155A

Plasmid

#72702

Purpose

Lentiviral expression of GR Phospho-deficient mutant (2nd generation)

Depositor

Freddy Jeanneteau

Article

Arango-Lievano et al Proc Natl Acad Sci U S A. 2015 Nov 16. p

Insert

GR (Nr3c1 Rat)

Use

Lentiviral

Tags

IRES-Venus

Expression

Mammalian

Mutation

S155A

Promoter

ubiquitin

Available Since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only
HL 3904 (=HL 770 + pHL 991)

Plasmid

#30093

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Insert

pLtetO-1:RhyB, pLlacO-1:hfq, pLtetO-1m9:sodB::gfp

Use

Synthetic Biology

Tags

GFP

Expression

Bacterial

Available Since

Sept. 9, 2011

Availability

Academic Institutions and Nonprofits only
pONL-N1_LAMP1

Plasmid

#65686

Purpose

Expresses LAMP1-ONL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

LAMP1

Use

Luciferase

Tags

ONL

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 15, 2016

Availability

Academic Institutions and Nonprofits only