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We narrowed to 26,606 results for: STI

Showing: 2841 - 2860 of 26606 results

  1. pCB’

    Plasmid
    #162703
    Purpose
    pCB reconstructed after selection for CCMB1 growth on glycerol under ambient air
    Depositor
    David Savage
    Article
    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1
    Insert
    pHnCB10 derived carboxysome operon, prk
    Use
    Tags
    Expression
    Bacterial
    Mutation
    5513T>G (tetR E37A); 7758G>T (second tet op…
    Promoter
    PLtet0-1 promoter
    Available Since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    162703-plasmid-map-sequence-id-324199

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  4. MSP1830

    Plasmid
    #70708
    Purpose
    Human expression plasmid for SaCas9 KKH variant: CAG-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Insert
    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Mammalian
    Mutation
    E782K, N968K and R1015H in SaCas9
    Promoter
    CAG
    Available Since
    Nov. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    70708-plasmid-map-sequence-id-364684

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  8. pShHELIX(Cas12k-TnsC)-sgRNA_entry (CJT113)

    Plasmid
    #181792
    Purpose
    Expresses 3-component ShHELIX containing a Cas12k-TnsC fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShTnsB, ShTniQ, ShCas12k-ShTnsC
    Use
    Tags
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    181792-plasmid-map-sequence-id-358226

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  13. MSP469

    Plasmid
    #65771
    Purpose
    Human expression vector for SpCas9 VQR variant: CMV-T7-humanSpCas9(D1135V/R1335Q/T1337R)-NLS-3xFLAG (VQR variant)
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/R1335Q/T1337R)-NLS-3XFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135V, R1335Q, and T1337R mutations in Cas9
    Promoter
    CMV & T7
    Available Since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65771-plasmid-map-sequence-id-112653

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  14. hCD12

    Plasmid
    #225541
    Purpose
    Expresses human histone deacetylase 6 (HDAC6) catalytic domain 1 and 2 (CD12), also known as hCD12, in Escherichia coli cells.
    Depositor
    David Christianson
    Article
    Hai et al Nat Chem Biol. 2016 Sep;12(9):741-7. doi
    Insert
    Human Histone Deacetylase 6 Catalytic Domain 1 and 2 (HDAC6 Human)
    Use
    Tags
    His6-MBP tag with cleavable TEV site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    Sept. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    225541-plasmid-map-sequence-id-446604

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  15. MSP712

    Plasmid
    #65768
    Purpose
    Bacterial expression plasmid for Sp-dCas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSpdCas9(D10A/H840A)-T7-BsaIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes dCas9 (D10A/H840A)-NLS-3XFlag, and SpCas9 gRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Bacterial
    Mutation
    D10A and H840A mutations in Cas9
    Promoter
    T7 (x2)
    Available Since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65768-plasmid-map-sequence-id-112655

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  16. BPK2101

    Plasmid
    #65770
    Purpose
    Bacterial expression plasmid for S. aureus Cas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSaCas9-NLS-3xFLAG-T7-BsaIcassette-Sa-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized SaCas9, and SaCas9 gRNA
    Use
    CRISPR
    Tags
    NLS-3xFlag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 (x2)
    Available Since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65770-plasmid-map-sequence-id-112650

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  17. MSP680

    Plasmid
    #65772
    Purpose
    Human expression vector for SpCas9 EQR variant: CMV-T7-humanSpCas9(D1135E/R1335Q/T1337R)-NLS-3xFLAG (EQR variant)
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E/R1335Q/T1337R)-NLS-3XFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135E, R1335Q, and T1337R mutations in Cas9
    Promoter
    CMV & T7
    Available Since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65772-plasmid-map-sequence-id-112654

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  18. pCMV-T7-hAcrVA1-NLS(sv40) (BPK5050)

    Plasmid
    #115136
    Purpose
    CMV-T7 promoter expression plasmid for human codon optimized AcrVA1 anti-CRISPR protein with C-terminal NLS
    Depositor
    Joseph Bondy-Denomy, Benjamin Kleinstiver, Keith Joung
    Article
    Marino et al Science. 2018 Sep 6. pii: science.aau517
    Insert
    human codon optimized AcrVA1 anti-CRISPR protein
    Use
    Tags
    NLS(SV40)
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    115136-plasmid-map-sequence-id-220499

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  19. SuperClomeleon

    Plasmid
    #203724
    Purpose
    An improved engineered fluorescent ratiometric optogenetic sensor to detect inhibitory synaptic activity via changes in intracellular chloride
    Depositor
    George Augustine
    Article
    Grimley et al J Neurosci. 2013 Oct 9;33(41):16297-309.
    Insert
    Cerulean linked topaz fluorescent protein
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Cerulean, modified Topaz Fluorescent Protein with…
    Promoter
    Available Since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    203724-plasmid-map-sequence-id-403640

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Showing: 2841 - 2860 of 26606 results