Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 11,800 results for: emb

Clear keywords
Showing: 2841 - 2860 of 11800 results

  6. pMS20: pHelper(PmcCAST)_PmcPSP1_ΔTnsD

    Plasmid
    #168153
    Purpose
    Inducible expression of PmcCAST proteins (except TnsD) with crRNA targeting PmcPSP1.
    Depositor
    Feng Zhang
    Publication
    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.
    Inserts
    PmcCAST minimal CRISPR array (with spacer for PmcPSP1)
    PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
    PmcCAST Tns proteins (TnsAB and TnsC)
    PmcTniQ
    Expression
    Bacterial
    Available since
    June 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    168153-plasmid-map-sequence-id-330740

    Enlarge

  7. pMS18: pHelper(PmcCAST)_PmcPSP1

    Plasmid
    #168151
    Purpose
    Inducible expression of PmcCAST proteins with crRNA targeting PmcPSP1.
    Depositor
    Feng Zhang
    Publication
    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.
    Inserts
    PmcCAST minimal CRISPR array (with spacer for PmcPSP1)
    PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
    PmcCAST Tns proteins (TnsAB, TnsC and TnsD)
    PmcTniQ
    Expression
    Bacterial
    Available since
    June 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    168151-plasmid-map-sequence-id-330728

    Enlarge

  9. pGBW-m4134357

    Plasmid
    #152589
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHis-003
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    July 30, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152589-plasmid-map-sequence-id-317728

    Enlarge

  10. pTO_Snf7_LAP

    Plasmid
    #101851
    Purpose
    Tet-inducible expression of Snf7 from S. cerevisiae with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration
    Depositor
    Daniel Gerlich
    Publication
    Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d
    Insert
    SNF7 (SNF7 Budding Yeast)
    Use
    Gateway cloning, flp-frt recombination, tet-induc…
    Tags
    Localization and Affinity Purification (LAP) tag
    Expression
    Bacterial and Mammalian
    Promoter
    CMV
    Available since
    Dec. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    101851-plasmid-map-sequence-id-200251

    Enlarge

  11. CibN

    Plasmid
    #209671
    Purpose
    Expresses CIB1 fused with Split-TurboID-N and pmGFP in mammalian cells
    Depositor
    Sanjeevi Sivasankar
    Publication
    Shafraz et al J Cell Sci. 2023 Oct 1;136(19):jcs261430
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CIB1 (CIB1 Mustard Weed)
    Tags
    Split-TurboID-N and plasma membrane-targeted EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    209671-plasmid-map-sequence-id-415908

    Enlarge

  12. HA-BACE1-mBFP

    Plasmid
    #196697
    Purpose
    Expresses Bace1 with an HA tag at N-terminal for single molecule tracking and other measusers in mammalian cells
    Depositor
    Martino Calamai
    Publication
    Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Beta-secretase 1 (BACE1 Human)
    Tags
    HA and mBFP
    Expression
    Mammalian
    Available since
    April 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    196697-plasmid-map-sequence-id-386452

    Enlarge

  13. pCS2+-Pard3-EGFP-PIF6

    Plasmid
    #154914
    Purpose
    Full length zebrafish Pard3 (Gene ID: 403050), tagged with EGFP fluorescent protein and Arabidopsis PIF6 (as in construct Addgene ID 154913).
    Depositor
    Clare Buckley, Jonathan Clarke
    Publication
    Buckley et al Dev Cell. 2016 Jan 11;36(1):117-26. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    par-3 family cell polarity regulator alpha, b (pard3ab Zebrafish)
    Use
    Xenopus/avian/zebrafish
    Tags
    1-100 amino acids of Arabidopsis PIF6 and EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    154914-plasmid-map-sequence-id-308721

    Enlarge

  14. pTagGFP2-N SIN1

    Plasmid
    #124919
    Purpose
    For expression of wild-type SIN1-GFP
    Depositor
    Jin Chen
    Publication
    Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MAPKAP1 (MAPKAP1 Human)
    Tags
    TagGFP2
    Expression
    Mammalian
    Mutation
    Insert ORF was sirently mutated to be resistent t…
    Available since
    May 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    124919-plasmid-map-sequence-id-244573

    Enlarge

  15. pEGFP-N1 VAMP2 R125TAG

    Plasmid
    #69876
    Purpose
    express in mammalian cells for unnatural amino acid incorporation into VAMP2-GFP
    Depositor
    Silvio Rizzoli
    Publication
    Vreja et al ACS Nano. 2015 Nov 24;9(11):11034-41. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    VAMP2-GFP (Vamp2 Rat)
    Use
    Synthetic Biology
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Amber stop codon in the linker between VAMP2 and …
    Available since
    Jan. 25, 2016
    Availability
    Academic Institutions and Nonprofits only

  16. RAMA-bio-His

    Plasmid
    #50737
    Purpose
    Expresses enzymatically monobiotinylated full length RAMA ectodomain
    Depositor
    Gavin Wright
    Publication
    Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RAMA
    Tags
    ratCD4d3+4,biotinylation sequence, 6xHis
    Expression
    Mammalian
    Mutation
    all potential N-linked glycosylation sites (N-X-S…
    Promoter
    CMV
    Available since
    Feb. 24, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    50737-plasmid-map-sequence-id-76050

    Enlarge

  18. pRN3P-NLS-EGFP-deadBtgh-3NLS

    Plasmid
    #194470
    Purpose
    Encodes for catalytically inactive bacterial O-GlcNAcase BtGH84 fused to enhanced GFP and NLSs for effective nuclear localization, in backbone for T3 In Vitro Transcription.
    Depositor
    Mathieu Boulard
    Publication
    Formichetti et al Dev Cell 10 Jan 2024
    Insert
    BtGH84
    Use
    For in vitro transcription from t3 promoter after…
    Tags
    SV40 Nuclear Localization Signal, SV40 Nuclear Lo…
    Mutation
    Aspartic Acid 242 (in Dennis et al.) changed to A…
    Available since
    Jan. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    194470-plasmid-map-sequence-id-384877

    Enlarge

  19. pTK165_Mem-mCherry-4.1G-CTD

    Plasmid
    #46362
    Depositor
    Iain Cheeseman
    Publication
    Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    4.1G C-terminal domain (EPB41L2 Human)
    Tags
    Mem (N-terminal 20 amino acids of neuromodulin fo…
    Expression
    Mammalian
    Mutation
    C-terminal domain from 886-1005 amino acids
    Promoter
    CMV
    Available since
    Aug. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    46362-plasmid-map-sequence-id-65994

    Enlarge

  20. hSyn ArcLightCo (Q239)-T2A-nls-mCherry

    Plasmid
    #85844
    Purpose
    Genetically encoded voltage sensor ArcLight- codon optimized
    Depositor
    Vincent Pieribone
    Publication
    Platisa et al ACS Chem Neurosci. 2017 Jan 3. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ArcLightCo-Q239
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Ci-VSP contains R217Q mutation; super ecliptic pH…
    Promoter
    hSyn1
    Available since
    Jan. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    85844-plasmid-map-sequence-id-170992

    Enlarge

Showing: 2841 - 2860 of 11800 results