We narrowed to 39,967 results for: lat

1. pUC-hU6-2O-crScaffold_SV40-BFP

   Plasmid

   #224861

   Purpose

   crRNA scaffold for RfxCas13d expressed from hU6-2xTetO promoter and reporter BFP protein expressed from SV40 promoter

   Depositor

   Gabor Balazsi

   Article

   Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   hU6-2xTetO

   Available Since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC-hU6-2O-crScaffold_EF1a-BFP

   Plasmid

   #224862

   Purpose

   crRNA scaffold for RfxCas13d expressed from hU6-2xTetO promoter and reporter BFP protein expressed from EF1a promoter

   Depositor

   Gabor Balazsi

   Article

   Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   hU6-2xTetO

   Available Since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. LPUtopia-8

   Plasmid

   #224781

   Purpose

   Landing Pad donor vector for AAVS1 site integration in human and VeroE6 cells

   Depositor

   Gabor Balazsi

   Article

   Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

   Inserts

   puromycin N-acetyltransferase
   enhanced Green Fluorescence Protein
   Red Fluorescence Protein
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUB_SM-FLuc0_EMCV-Ren

   Plasmid

   #210590

   Purpose

   Expresses SM(FLAG)-nonoptimal Firefly luciferase, and EMCVIRES-driven Renilla luciferase

   Depositor

   Olivia Rissland

   Article

   Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

   Inserts

   Spaghetti monster (FLAG)-nonoptimal FLuc
   Optimal Renilla luciferase
   

   Use

   Luciferase

   Tags

   Spaghetti monster (FLAG)

   Expression

   Mammalian

   Promoter

   EMCV IRES and UBC human

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUB_SM-FLuc100_EMCV-Ren

   Plasmid

   #210591

   Purpose

   Expresses SM(FLAG)-optimal Firefly luciferase, and EMCVIRES-driven Renilla luciferase

   Depositor

   Olivia Rissland

   Article

   Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

   Inserts

   Spaghetti monster (FLAG)-optimal FLuc
   Optimal Renilla luciferase
   

   Use

   Luciferase

   Tags

   Spaghetti monster (FLAG)

   Expression

   Mammalian

   Promoter

   EMCV IRES and UBC human

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGFP-CUG-IRES-mCherry

   Plasmid

   #222110

   Purpose

   Start codon reporter with mutated start codon

   Depositor

   Jonathan Weissman

   Article

   She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

   Insert

   GFP-IRES-mCherry
   

   Use

   Lentiviral

   Mutation

   CUG start codon

   Available Since

   Oct. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGFP-ACG-IRES-mCherry

   Plasmid

   #222113

   Purpose

   Start codon reporter with mutated start codon

   Depositor

   Jonathan Weissman

   Article

   She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

   Insert

   GFP-IRES-mCherry
   

   Use

   Lentiviral

   Mutation

   ACG start codon

   Available Since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGFP-UUG-IRES-mCherry

   Plasmid

   #222112

   Purpose

   Start codon reporter with mutated start codon

   Depositor

   Jonathan Weissman

   Article

   She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

   Insert

   GFP-IRES-mCherry
   

   Use

   Lentiviral

   Mutation

   UUG start codon

   Available Since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGFP-AUU-IRES-mCherry

   Plasmid

   #222118

   Purpose

   Start codon reporter with mutated start codon

   Depositor

   Jonathan Weissman

   Article

   She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

   Insert

   GFP-IRES-mCherry
   

   Use

   Lentiviral

   Mutation

   AUU start codon

   Available Since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGFP-AGG-IRES-mCherry

    Plasmid

    #222115

    Purpose

    Start codon reporter with mutated start codon

    Depositor

    Jonathan Weissman

    Article

    She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

    Insert

    GFP-IRES-mCherry
    

    Use

    Lentiviral

    Mutation

    AGG start codon

    Available Since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGFP-AUC-IRES-mCherry

    Plasmid

    #222117

    Purpose

    Start codon reporter with mutated start codon

    Depositor

    Jonathan Weissman

    Article

    She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

    Insert

    GFP-IRES-mCherry
    

    Use

    Lentiviral

    Mutation

    AUC start codon

    Available Since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTE5423_p3WJdB-glcO60

    Plasmid

    #221194

    Purpose

    DNA template of GlcR sensor with glcO60 operator: T7 promoter-glcO60-3WJdB reporter-T7 terminator linear DNA template (PCR amplified with oSB0021+22) is used in the IVT sensor system

    Depositor

    Tobias Erb

    Article

    Barthel et al Synth Biol (Oxf). 2024 Sep 20;9(1):ysae0

    Insert

    T7 promoter-glcO60-3WJdB
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    T7 promoter with downstream glcO60 operator

    Available Since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. SUMO-LPQTG-pET

    Plasmid

    #221851

    Purpose

    Expresses SUMO-2 with sortase motif and His tag

    Depositor

    Artur Krężel

    Article

    Antonenko et al ACS Omega. 2024 Oct 30;9(45):45127-45137

    Insert

    SUMO-2 (SUMO2 Human)
    

    Expression

    Bacterial

    Mutation

    Gln88Leu, Gln89Pro

    Available Since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMRXIP-GFP-PLCd1(PHD)

    Plasmid

    #221753

    Purpose

    Stable expression of GFP-PLCd1(PHD) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    PLCd1 (PLCD1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    amino acids 1–175

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMRXIP-GFP-2xFYVE

    Plasmid

    #221750

    Purpose

    Stable expression of GFP-2xFYVE in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    tandem HRS FYVE domain
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    two FYVE domains (amino acids 147-223), linked by…

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBabe-Sik-YF

    Plasmid

    #210723

    Purpose

    Construct for stable expression of constitutively active mouse Ptk6 (Sik) in mammalian cells.

    Depositor

    Angela Tyner

    Article

    Derry et al Mol Cell Biol. 2000 Aug;20(16):6114-26.

    Insert

    Protein tyrosine kinase 6 (Ptk6 Mouse)
    

    Expression

    Mammalian

    Mutation

    Tyrosine 447 changed to Phenylalanine

    Available Since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQE60 G121A wgMDH

    Plasmid

    #204252

    Purpose

    Bacterial expression of G121A wgMDH with IPTG induction - Active site loop mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    G121A

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pQE60 R196E wgMDH

    Plasmid

    #204272

    Purpose

    Bacterial expression of R196E wgMDH with IPTG induction - Active site mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    R196E

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pQE60 P123A wgMDH

    Plasmid

    #204254

    Purpose

    Bacterial expression of P123A wgMDH with IPTG induction - Active site loop mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    P123A

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pQE60 R130Q wgMDH

    Plasmid

    #204268

    Purpose

    Bacterial expression of R130Q wgMDH with IPTG induction - Active site mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    R130Q

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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