We narrowed to 18,361 results for: met

1. UMG-SP-3_pET29b

   Plasmid

   #215435

   Purpose

   Expression construct for UMG-SP-3 gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   UMG-SP-3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCry2PHR-mCh-LRP6c

   Plasmid

   #42960

   Purpose

   Expresses optogenetically controlled LRP6 (C-terminal domain)

   Depositor

   David Schaffer

   Article

   Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

   Insert

   Cry2PHR-mCherry-LRP6c
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   LPR6 cytoplasmic domain only

   Promoter

   CMV

   Available Since

   April 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pACU2_CD4-mIFP T2A HO1

   Plasmid

   #72441

   Purpose

   Expressing UAS-CD4-mIFP T2A HO1 in drosophila

   Depositor

   Xiaokun Shu

   Article

   Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

   Insert

   CD4-mIFP T2A HO1
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   March 29, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pRSETA-qmTQ2-ATP

   Plasmid

   #225247

   Purpose

   Bacterial expression of N-terminal His-tagged qmTQ2-ATP, a cyan fluorescent FLIM biosensor for ATP imaging

   Depositor

   Yasushi Okada

   Article

   Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

   Insert

   qmTQ2-ATP
   

   Use

   Tags

   6XHis, T7 tag, XpressTM tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. GAL-SUV39H2-L

   Plasmid

   #89136

   Purpose

   mammalian expression of full-length 410 amino acid SUV39H2 histone methyltransferase linked to GAL4 DNA binding domain

   Depositor

   Elizabeth Wilson

   Article

   Askew et al Mol Cell Endocrinol. 2017 Mar 5;443:42-5

   Insert

   SUV39H2 (SUV39H2 Human)
   

   Use

   Tags

   GAL4 DBD

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PARP15-c020

   Plasmid

   #173101

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   PARP15 (PARP15 Human)
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   N78-S267, codon optimized

   Promoter

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. modRNAc0-p53DD

   Plasmid

   #176902

   Purpose

   IVT template plasmid for making p53DD modRNA

   Depositor

   Xiaojun Lian

   Article

   Haideri et al Cell Rep Methods. 2022 Sep 7;2(9):100290

   Insert

   p53DD
   

   Use

   Ivt template plasmid

   Tags

   Expression

   Mutation

   Promoter

   T7 promoter

   Available Since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PARP15-c021

   Plasmid

   #173102

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   PARP15 (PARP15 Human)
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   G287-N470, codon optimized

   Promoter

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GI-Gal4 DBD

   Plasmid

   #42500

   Purpose

   GIGANTEA fused with Gal4 DNA binding domain (DBD), part of a light-inducible transcription factor

   Depositor

   Ricardo Dolmetsch

   Article

   Yazawa et al Nat Biotechnol. 2009 Oct;27(10):941-5. d

   Insert

   GIGANTEA fused with Gal4 DBD
   

   Use

   Tags

   Gal4 DNA binding domain (DBD)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mRuby2-Rab5a-7

    Plasmid

    #55911

    Purpose

    Localization: Ras GTPase, Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    Rab5a
    

    Use

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 4, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pTriEx-mCherry-LOV2 V416L

    Plasmid

    #81036

    Purpose

    mammalian expression of LOV V416L, a mutant with super long relaxation time

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    LOV V416L
    

    Use

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    V416L

    Promoter

    Available Since

    Aug. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCDH_Str-KDEL_neomycin

    Plasmid

    #65307

    Purpose

    Luminal ER hook only (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Streptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end
    

    Use

    Lentiviral

    Tags

    KDEL motif and Signal Sequence

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pIntegrase_4

    Plasmid

    #60576

    Purpose

    plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Integrase_4
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pBAD

    Available Since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA-mCherry-4A/4B-CAAX

    Plasmid

    #87699

    Purpose

    Express mCherry with a peptide linker (contaning a HCVp 4A/4B substrate site) and a C-terminal CAAX box from KRras for plasma-membrane targeting in mammalian cells

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    mCherry-4A/4B-CAAX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CL7-LbCas12a

    Plasmid

    #164658

    Purpose

    Bacterial expression of LbCas12a using CL7/Im7

    Depositor

    Piyush Jain

    Article

    Nguyen et al Methods. 2021 Feb 9. pii: S1046-2023(21)

    Insert

    LbCas12a
    

    Use

    Tags

    Thioredoxin, CL7, SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFGC5941-PacI-ath-STTM156/157

    Plasmid

    #84083

    Purpose

    target miRNA156/157 for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM156/157
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S

    Available Since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFGC5941-PacI-ath-STTM160

    Plasmid

    #84085

    Purpose

    target miRNA160 for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM160
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-ADP-MCS-FLAG

    Plasmid

    #192360

    Purpose

    Adipocyte-specific overexpression of genes

    Depositor

    Yifu Qiu

    Article

    Xue et al Cell Metab. 2022 Sep 6;34(9):1325-1341.e

    Type

    Empty backbone

    Use

    AAV

    Tags

    3xflag

    Expression

    Mammalian

    Mutation

    Promoter

    adiponectin promoter

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-cytoFLARE1.0/1.1-TF

    Plasmid

    #234516

    Purpose

    Transcriptional reporter for detecting calcium transients in neuronal cultures (transcription factor)

    Depositor

    Wenjing Wang

    Article

    Zhou et al Cell Rep Methods. 2025 Apr 21;5(4):10101

    Insert

    ERT2-MK2-hLOV-TEVcs-FLAG-tTA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 30, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pFGC5941DR5-PacI-ath-STTM164

    Plasmid

    #84090

    Purpose

    target miRNA164 for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM164
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S

    Available Since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge