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We narrowed to 18,311 results for: puro

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Showing: 2841 - 2860 of 18311 results

  1. pLentiRNACRISPR_002 - hU6-DR_BsmBI-EFS-PguCas13b-NES-2A-Puro-WPRE

    Plasmid
    #138144
    Purpose
    Expresses PguCas13b in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang cacc. R overhang caac
    Depositor
    Neville Sanjana
    Article
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    PguCas13b
    Use
    Lentiviral
    Tags
    NES
    Available Since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    138144-plasmid-map-sequence-id-272529

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  4. loxP-Puro-loxP-3xV5-SNAP-Rab11 HR

    Plasmid
    #229677
    Purpose
    Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with SNAP tag and a 3x V5 epitope tag. Contains a puromycin resistance cassette for selection of edited cells.
    Depositor
    Francesca Bottanelli
    Article
    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/
    Insert
    genomic sequence Rab11 locus (RAB11A Human)
    Use
    CRISPR
    Tags
    SNAP, 3xV5
    Available Since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    229677-plasmid-map-sequence-id-457129

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  6. pLenti-C-Myc-DDK-IRES-Puro METTL3

    Plasmid
    #200724
    Purpose
    Lentiviral expression of Flag-Tagged human METTL3
    Depositor
    Claudio Alarcón
    Insert
    METTL3 (METTL3 Human)
    Use
    Lentiviral
    Tags
    Myc-DDK from backbone plasmid
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    200724-plasmid-map-sequence-id-401795

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  16. TRIPZ-bioGEF-SUMO1nc-PURO

    Plasmid
    #208042
    Purpose
    Enables inducible expression of low-affinity Avitag-SUMO1nc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin
    Depositor
    Rosa Barrio, James Sutherland
    Article
    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:
    Insert
    SUMO1nc (SUMO1 Human)
    Use
    Lentiviral
    Tags
    Low-affinity Avitag
    Mutation
    Q94P Mutation near C-terminus suppresses cleavage…
    Promoter
    tetO/UBC
    Available Since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208042-plasmid-map-sequence-id-410263

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  17. TRIPZ-bioGEF-SUMO2nc-PURO

    Plasmid
    #208043
    Purpose
    Enables inducible expression of low-affinity Avitag-SUMO2nc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin
    Depositor
    Rosa Barrio, James Sutherland
    Article
    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:
    Insert
    SUMO2nc (SUMO2 Human)
    Use
    Lentiviral
    Tags
    Low-affinity Avitag
    Mutation
    Q90P Mutation near C-terminus suppresses cleavage…
    Promoter
    tetO/UBC
    Available Since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208043-plasmid-map-sequence-id-410267

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Showing: 2841 - 2860 of 18311 results