We narrowed to 78,677 results for: TRI

1. pCMV4a-SIRT6-H133Y-FLAG

   Plasmid

   #102324

   Purpose

   Human SIRT6 H133Y with FLAG tag for mammalian expression

   Depositor

   Hening Lin

   Article

   Zhang et al Nat Chem Biol. 2016 Aug;12(8):614-20. do

   Insert

   SIRT6 (SIRT6 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed histidine 133 to tyrosine

   Promoter

   CMV

   Available since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1_mGFP-FGFR3

   Plasmid

   #191752

   Purpose

   Expression of N-terminally mGFP labelled wildtype FGFR3 (isoform IIIc)

   Depositor

   Irene Tiemann-Boege

   Article

   Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi

   Insert

   mGFP-FGFR3IIIc
   

   Use

   Tags

   mGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. p5M-hPR-B

   Plasmid

   #89114

   Purpose

   mammalian expression of full-length human progesterone receptor-B in pCMV5 with 5 restriction sites deleted in polylinker

   Depositor

   Elizabeth Wilson

   Article

   Su et al J Biol Chem. 2012 Oct 5;287(41):34809-24

   Insert

   human progesterone receptor-B (PGR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   5 restriction sites deleted in polylinker

   Promoter

   CMV

   Available since

   April 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC19-U6-LwaCas13acrRNA-BsmBIcassette (LTH151)

   Plasmid

   #171129

   Purpose

   LwaCas13a crRNA entry vector for pU6 expression (need to clone in spacer into BsmBI sites)

   Depositor

   Benjamin Kleinstiver

   Article

   Johnson et al Mol Cell. 2022 Jun 2;82(11):2161-2166.e3

   Insert

   U6 promoter entry plasmid for LwaCas13a crRNA cloning
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. p8103 LentiCRISPR v2 sgNT-2

   Plasmid

   #163313

   Purpose

   Expresses Cas9 and a non-targeting control guide RNA

   Depositor

   Elizabeth White

   Article

   Hatterschide et al Proc Natl Acad Sci U S A. 2019 Apr 2;116

   Insert

   sgNT-2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-Akt(K179M)

   Plasmid

   #39535

   Depositor

   Julian Downward

   Article

   Watton et al Curr Biol. 1999 Apr 22;9(8):433-6.

   Insert

   Akt(K179M) (Akt1 Mouse)
   

   Use

   Tags

   6G linker, EGFP, and HA

   Expression

   Mammalian

   Mutation

   K179M

   Promoter

   Available since

   Sept. 26, 2012

   Availability

   Academic Institutions and Nonprofits only

7. Twitch-4 pcDNA3

   Plasmid

   #49533

   Purpose

   Fluorescent reporter for calcium signaling

   Depositor

   Oliver Griesbeck

   Article

   Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

   Insert

   Twitch-4
   

   Use

   Tags

   ECFP and cpCit174

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pCMV_nLightR

   Plasmid

   #217657

   Purpose

   Expresses norepinephrine indicator nLightR under mammalian promoter

   Depositor

   Tommaso Patriarchi

   Article

   Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

   Insert

   nLightR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-hSyn-Gβγ-BERKY1

   Plasmid

   #158429

   Purpose

   BRET biosensor for detection of free Gβγ in lentiviral vector pLenti-hSyn

   Depositor

   Mikel Garcia-Marcos

   Article

   Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

   Insert

   Lyn11-Nluc-ER/K linker-YFP-GRK3ct
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSynapsin

   Available since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTK47_mCherry-LGN-hardened

    Plasmid

    #46347

    Purpose

    rescues the phenotypes caused by LGN RNAi

    Depositor

    Iain Cheeseman

    Article

    Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

    Insert

    LGN (GPSM2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    siRNA resistant, gaGTtGacTgcTcgTTtA; deletion of …

    Promoter

    CMV

    Available since

    Aug. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-rTH-tTA

    Plasmid

    #133268

    Purpose

    An AAV genome that expresses tTA from the rat TH promoter

    Depositor

    Viviana Gradinaru

    Article

    Robinson et al Elife. 2019 Sep 23;8. pii: 48983. doi: 1

    Insert

    tTA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    *(see below)

    Promoter

    rTH

    Available since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLVX Flag RagC WT

    Plasmid

    #112756

    Purpose

    express Flag-RagC

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    Flag-RagC (RRAGC Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Available since

    Oct. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. myc-BioID-Rab7

    Plasmid

    #121049

    Purpose

    Expresses myc tagged BioID and human Rab7a in mammalian cells

    Depositor

    Gia Voeltz

    Article

    Hoyer et al Cell. 2018 Sep 20;175(1):254-265.e14. do

    Inserts

    BioID
    Rab7A
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    R118G (highly promiscuous form)

    Promoter

    CMV

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMuLE_ENTR_TOP-NLuc1.1_L5-L4

    Plasmid

    #113867

    Purpose

    Single pathway reporter; wnt promoter-driven expression of intracellular NanoLuc; MultiSite Gateway entry vector.

    Depositor

    Manfred Ogris

    Article

    Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

    Inserts

    TOP (wnt dependent promoter)
    NLuc
    

    Use

    Mule gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-EF1a-DIO-APEX.NES-P2A-EGFP

    Plasmid

    #182824

    Purpose

    Cre-dependent expression of cytosolic APEX

    Depositor

    Yevgenia Kozorovitskiy

    Article

    Dumrongprechachan et al Nat Commun. 2021 Aug 11;12(1):4855. doi:

    Insert

    APEX.NES-P2A-EGFP
    

    Use

    AAV

    Tags

    EGFP reporter (P2A)

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-Nrxn1beta AS4(-)-Fc

    Plasmid

    #59313

    Purpose

    Expression plasmid for secreted Fc-fusion of mouse Neurexin 1beta AS4(-) [lacking the insert at alternatively spliced segment 4 (AS4-)]

    Depositor

    Tito Serafini, Peter Scheiffele

    Article

    Scheiffele et al Cell. 2000 Jun 9. 101(6):657-69.

    Insert

    Nrxn1beta AS4(-)-Fc fusion (Nrxn1 Mouse)
    

    Use

    Tags

    Fc (from human IgG)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pIA1363

    Plasmid

    #166861

    Purpose

    Expression of codon optimized SARS-COV-2 Nsp8 in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

    Insert

    SARS-COV-2 Nsp8 (ORF1ab Synthetic)
    

    Use

    Tags

    His-GB1

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pIA1362

    Plasmid

    #166860

    Purpose

    Expression of codon optimized SARS-COV-2 Nsp7 in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

    Insert

    SARS-COV-2 Nsp7 (ORF1ab Synthetic)
    

    Use

    Tags

    His-GB1

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. RINS1(mut)

    Plasmid

    #107291

    Purpose

    Plasmid for the expression of the inactive mutant of RINS1 sensor

    Depositor

    Dmytro Yushchenko

    Article

    Schifferer et al Cell Chem Biol. 2017 Apr 20;24(4):525-53

    Insert

    RINS1(mut)
    

    Use

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    two Arg/Ser mutations at the beginning and in the…

    Promoter

    CMV

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. PROM_MpUBE2

    Plasmid

    #136109

    Purpose

    PROM for cloning with 5UTR_MpUBE2. PROM+5UTR_MpUBE2 has ubiquitous expression in early satges of gemmae development.

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    PROM_MpUBE2 Mapoly0106s0019
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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21. 5UTR_MpUBE2

    Plasmid

    #136110

    Purpose

    5UTR for cloning with PROM_MpUEB2. PROM+5UTR_MpUBE2 has ubiquitous expression in early stages of gemmae development.

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    5UTR_MpUBE2 Mapoly0106s0019
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. p3xFLAG-CMV14-mULK1

    Plasmid

    #24301

    Depositor

    Noboru Mizushima

    Article

    Hosokawa et al Mol Biol Cell. 2009 Apr . 20(7):1981-91.

    Insert

    a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

23. pcDNA3.1 Flag-Sesn2

    Plasmid

    #61868

    Purpose

    Transient overexpression of Flag-Sesn2 (mouse)

    Depositor

    Ming Li

    Article

    Peng et al Cell. 2014 Sep 25;159(1):122-33. doi: 10

    Insert

    Sesn2 (Sesn2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

24. pRcCMV Cep164 (Nigg CW325)

    Plasmid

    #41148

    Depositor

    Erich Nigg

    Article

    Graser et al J Cell Biol. 2007 Oct 22;179(2):321-30.

    Insert

    Cep164 (CEP164 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

25. pcDNA3-AU1-mTOR-L1460P

    Plasmid

    #19995

    Depositor

    Fuyuhiko Tamanoi

    Article

    Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

    Insert

    mammalian target of rapamycin-L1460P (Mtor Rat)
    

    Use

    Tags

    AU1

    Expression

    Mammalian

    Mutation

    Changed Leucine 1460 to Proline

    Promoter

    Available since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

26. pMRX-INU-TEX264 LIR4A-FLAG

    Plasmid

    #128259

    Purpose

    Expresses TEX264 LIR4A in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

    Insert

    TEX264 LIR4A (TEX264 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Changed FEEL (aa 273-276) to alanines

    Promoter

    CMV

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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27. pLenti-CMV-CAG-GFP-EnvA

    Plasmid

    #102987

    Purpose

    Lentivirus expressing eGFP and (EnvA-B19 Rabies Glycoprotein) fusion protein. Used to package EnvA pseudotyped G-deleted Rabies

    Depositor

    Marco Tripodi

    Article

    Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306

    Inserts

    Enhanced Green Fluorescent Protein (eGFP)
    EnvA-B19 Rabies Glycoprotein
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGREAT26

    Plasmid

    #170914

    Purpose

    GoldenBraid Double Transcriptional Unit - pEstradiol_Inducible_E-Luc_NtEUt+pNOS_Red-F_NOSt

    Depositor

    Markita Landry

    Article

    Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

    Insert

    E-Luc
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    c.375C>T silent mutation to remove BbsI site (…

    Promoter

    pEstradiol_Inducible

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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29. pcDNA3-Nrxn1beta AS4(+)-Fc

    Plasmid

    #59314

    Purpose

    Expression plasmid for secreted Fc-fusion of mouse Neurexin 1beta AS4(+) [containing the insert at alternatively spliced segment 4 (AS4+)]

    Depositor

    Tito Serafini, Peter Scheiffele

    Article

    Scheiffele et al Cell. 2000 Jun 9. 101(6):657-69.

    Insert

    Nrxn1beta AS4(+)-Fc fusion (Nrxn1 Mouse)
    

    Use

    Tags

    Fc (from human IgG)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCS2+PIF6CreN

    Plasmid

    #131780

    Purpose

    Mammalian, Zebrafish, or Xenopus expression of PIF6CreN, one half of the CreLite system

    Depositor

    George Eisenhoffer

    Article

    Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

    Insert

    PIF6CreN
    

    Use

    Cre/Lox; Zebrafish expression

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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